21,167 results
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Plasmid#122522PurposeExpression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesisDepositorInsertgp43 - DNA polymerase (43 Escherichia virus T4)
ExpressionBacterialMutationtyrosine 320 changed to alaninePromoterT7 promoterAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
EC1000
Bacterial Strain#71852PurposeRepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmidsDepositorBacterial ResistanceKanamycinAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC-CANTHipi
Plasmid#53301PurposeContains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.DepositorInsertcrtO
UseLow copy number bacterial cloning vectorTags6HisPromoterTrcAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-EBF1
Plasmid#96965PurposeDox-inducible expression of Ebf1 in mammalian cellsDepositorAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET30b-7+-SrtA
Plasmid#105602Purposeexpressed 7+ sortase A variant in E.coliDepositorInsertS.aureus Sortase A, 7+ mutant
TagsHis tagExpressionBacterialPromoterT7Available SinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-mSmo
Plasmid#25395DepositorAvailable SinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(1)-T2A-Gal4DBD-Hsp70
Plasmid#62903PurposeCreating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1DepositorInsertT2A-Gal4DBD-Hsp70
Available SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI-SEP_NR2A
Plasmid#23997DepositorAvailable SinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_NbH11-H4
Plasmid#155364PurposeExpresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2DepositorInsertH11-H4
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
(559) pAAV EF-1a KRAB-SadCas9 U6-gRNA
Plasmid#163024PurposeExpression of CRISPRi with U6-gRNA (Bsa1 sites)DepositorInsertMammalian codon-optimized SadCas9
UseAAVTagsKRAB, SV40 polyA, and myc NLSExpressionMammalianPromoterhEF-1aAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only