Showing: 1 - 20 of 21 results
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Plasmid#131660PurposeBacterial expression of ETS gene EHFDepositorInsertEHF (EHF Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEHF-donor
Plasmid#113817PurposeCRISPR donor plasmid to tag human transcription factor EHF with GFPDepositorInsertEHF homology arms flanking EGFP-IRES-Neo cassette (EHF Human)
UseCRISPRTagsExpressionMutationrs3818514, rs11032804, hg38:11:34659087:G>APromoterAvailabilityAcademic Institutions and Nonprofits only -
pEHF.1.0-gDNA
Plasmid#113785PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor EHFDepositorInsertEHF (EHF Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
TFORF2231
Plasmid#141979PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertEHF (EHF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
TFORF2232
Plasmid#141980PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertEHF (EHF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
TFORF2230
Plasmid#142187PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertEHF (EHF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
RhGC(BE)_pGEM
Plasmid#85469PurposeThe rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signalingDepositorInsertrhodopsin-guanylyl cyclase
UseTagsExpressionMammalianMutationPromoterT7 PromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP
Plasmid#35501PurposeAAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.DepositorInserthChR2
UseAAVTagsEYFPExpressionMammalianMutationC128S and D156APromoterCaMKIIaAvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 YFP-CaRhAC
Plasmid#101721PurposeYellow fluorescent protein (YFP) – tagged rhodopsin adenylyl cyclase created from the rhodopsin guanylyl cyclase of Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracelluDepositorInsertYFP CaRhAC
UseAAVTagsYFPExpressionMammalianMutationPromoterhSyn1AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 CaRhAC T2A tDimer
Plasmid#101722Purpose- humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neuronsDepositorInsertsCatRhAC
red fluorescent protein
UseAAVTagsExpressionMutationE497K,C569DPromoterhuman Synapsin1 promotor and ribosomal skip seque…AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer
Plasmid#66779PurposeThe rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signalingDepositorInsertshbeRhGC
red fluorescent protein
UseAAVTagsExpressionMammalianMutationPromoterhuman SynapsinAvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO C1V1 (t/t)-TS-EYFP
Plasmid#35497PurposeAAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.DepositorHas ServiceAAV9InsertChR1-VChR1 Chimera
UseAAVTagsEYFPExpressionMammalianMutationE122T and E162TPromoterEf1aAvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry
Plasmid#35498PurposeAAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.DepositorInsertChR1-VChR1 Chimera
UseAAVTagsmCherryExpressionMammalianMutationE122T and E162TPromoterEf1aAvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP
Plasmid#35499PurposeAAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.DepositorHas ServiceAAV9InsertChR1-VChR1 Chimera
UseAAVTagsEYFPExpressionMammalianMutationE122T and E162TPromoterCaMKIIaAvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-C1V1 (t/t)-TS-mCherry
Plasmid#35500PurposeAAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.DepositorInsertChR1-VChR1 Chimera
UseAAVTagsmCherryExpressionMammalianMutationE122T and E162TPromoterCaMKIIaAvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(C128S/D156A)-mCherry
Plasmid#35502PurposeAAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.DepositorInserthChR2(C128S/D156A)-mCherry
UseAAVTagsmCherryExpressionMammalianMutationC128S and D156APromoterCaMKIIaAvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO hChR2(C128S/D156A)-EYFP
Plasmid#35503PurposeAAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.DepositorInserthChR2(C128S/D156A)-EYFP
UseAAVTagsEYFPExpressionMammalianMutationC128S and D156APromoterEf1aAvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO hChR2(C128S/D156A)-mCherry
Plasmid#35504PurposeAAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.DepositorInserthChR2(C128S/D156A)-mCherry
UseAAVTagsmCherryExpressionMammalianMutationC128S and D156APromoterEf1aAvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 CaRhGC wt T2A tDimer
Plasmid#101720Purposehumanized, Neuron-specific promoter driven expression of the rhodopsin guanylyl cyclase from Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracellular cAMP close to the mDepositorInsertsCatRhGC
red fluorescent protein
UseAAVTagsExpressionMammalianMutationPromoterhSyn1 and ribosomal skip sequence T2AAvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP (AAV9)
Viral Prep#35499-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP (#35499). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-C1V1 (t/t)-TS-EYFP plasmid DNA. CaMKIIa-driven, C1V1 (t/t) fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsEYFPAvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 20 of 21 results