Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

166 results

Showing: 81 - 120 of 166 results

  1. MXS Chaining Kit

    Plasmid Kit
    #1000000064
    Purpose
    A collection of standardized building blocks used to assemble plasmids that are optimized for fluorescence imaging or flow cytometry applications.
    Depositor
    Pierre Neveu
    Application
    Cloning and Synthetic Biology
    Vector Type
    Mammalian Expression
    Cloning Type
    Restriction Enzyme
    Available Since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. Chromoprotein kit 1.0

    Plasmid Kit
    #1000000174
    Purpose
    Expression of visible chromoproteins in E. coli.
    Depositor
    Anthony Forster
    Application
    For the Classroom, Gene Expression and Labeling
    Vector Type
    Bacterial Expression
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only

  3. FusX TALEN assembly system

    Plasmid Kit
    #1000000063
    Purpose
    Streamlined Golden Gate TALE assembly of 15–16 RVD TALEN inserts in one step that is backwards compatible with popular TALE backbones for expression in multiple systems; requires additional plasmids for use.
    Depositor
    Stephen Ekker
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Zebrafish Expression
    Editing Type
    TALEN
    Cloning Type
    Golden Gate
    Available Since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. Winslow Lab Retroviral Barcoding Library

    Plasmid Kit
    #1000000097
    Purpose
    Plasmids with 6-nucleotide barcodes for in vivo phenotypic screening of small-molecule libraries.
    Depositor
    Monte Winslow
    Application
    Barcoding
    Vector Type
    Mammalian Expression, Retroviral
    Available Since
    Dec. 5, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. EasyClone 2.0 Yeast Toolkit

    Plasmid Kit
    #1000000073
    Purpose
    An expanded toolkit of integrative vectors for stable gene expression in S. cerevisiae.
    Depositor
    Irina Borodina
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    USER
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. MultiGreen

    Plasmid Kit
    #1000000250
    Purpose
    Modular assembly of multi-gene expression vectors.
    Depositor
    Wayne Parrott
    Application
    Cloning and Synthetic Biology
    Vector Type
    Plant Expression
    Cloning Type
    Golden Gate (GreenGate)
    Available Since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only

  7. CHLOROMODAS Kit

    Plasmid Kit
    #1000000269
    Purpose
    Plasmids for for engineering the Chlamydomonas reinhardtii chloroplast.
    Depositor
    Tobias Erb
    Application
    Cloning and Synthetic Biology
    Vector Type
    Algal Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Nov. 7, 2025
    Availability
    Academic Institutions and Nonprofits only

  8. AID* Kit

    Plasmid Kit
    #1000000120
    Purpose
    Plasmid for fusion of a protein of interest to the auxin-inducible degron to control protein levels in vivo.
    Depositor
    Helle Ulrich
    Application
    Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Available Since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  9. EasyCloneYALI- Integrative Vector Set

    Plasmid Kit
    #1000000140
    Purpose
    Integrative plasmids for overexpression of heterologous genes in Yarrowia lipolytica.
    Depositor
    Irina Borodina
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Cloning Type
    USER
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only

  10. EMMA toolkit

    Plasmid Kit
    #1000000119
    Purpose
    Collection of Golden Gate vectors and standardized genetic parts for the construction mammalian expression plasmids.
    Depositor
    Yizhi Cai
    Application
    Cloning and Synthetic Biology
    Vector Type
    Mammalian Expression
    Cloning Type
    Golden Gate
    Available Since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only

  11. Multiplex CRISPR dCas9/FokI-dCas9 Accessory Pack

    Plasmid Kit
    #1000000062
    Purpose
    Plasmids to create all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs for genome engineering using dCas9 and FokI-dCas9. The Multiplex CRISPR/Cas9 Assembly System Kit (kit #1000000055) is required for the use of this accessory pack.
    Depositor
    Takashi Yamamoto
    Application
    Genome Editing
    Vector Type
    Mammalian Expression
    Editing Type
    CRISPR
    Available Since
    June 1, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pMVP and pMAGIC Cloning Systems

    Plasmid Kit
    #1000000155
    Purpose
    For rapid modular cloning of adenoviral, lentiviral, PiggyBac and Sleeping Beauty transposon vectors for transgene or RNAi delivery (pMVP) and dCas9-based epigenetic engineering (pMAGIC).
    Depositor
    Christopher Newgard
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Adenoviral, Lentiviral, Mammalian Expression
    Editing Type
    CRISPR
    Cloning Type
    MultiSite Gateway
    Available Since
    Sept. 6, 2019
    Availability
    Academic Institutions and Nonprofits only

  13. MoClo Yeast Secretion Biosensor (YSB) Toolkit

    Plasmid Kit
    #1000000268
    Purpose
    Library of plasmids containing a variety of signal peptides, C-tags, and alpha-mating factor tags for use in protein secretion optimization in yeast.
    Depositor
    Joseph Brock
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only

  14. Fire Lab C. Elegans Kit

    Plasmid Kit
    #1000000001
    Purpose
    Large set of vectors for C. elegans research, including tools for RNAi, lacZ and/or fluorescent protein fusion vectors.
    Depositor
    Andrew Fire
    Application
    Gene Expression and Labeling
    Vector Type
    Worm Expression
    Available Since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  15. GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9

    Plasmid Kit
    #1000000154
    Purpose
    Targeted genome integration in human cells, zebrafish embryos, and pig fibroblasts.
    Depositor
    Jeffrey Essner
    Application
    Genome Editing
    Vector Type
    Mammalian Expression, Zebrafish Expression
    Editing Type
    CRISPR
    Available Since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only

  16. openCIDAR MoClo Toolkit

    Plasmid Kit
    #1000000227
    Purpose
    A collection of standardized parts compatible with CIDAR MoClo (Addgene #1000000059) to assemble gene expression constructs in many bacteria across Pseudomonadota.
    Depositor
    Eric Young
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only

  17. pCri System

    Plasmid Kit
    #1000000058
    Purpose
    Plasmids used for cytoplasmic and periplasmic/extracellular heterologous protein expression in E. coli, Bacillus subtilis, and Pichia pastoris.
    Depositor
    F Xavier Gomis-Ruth
    Application
    Gene Expression and Labeling
    Vector Type
    Bacterial Expression, Yeast Expression
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. Golden GATEway Cloning Kit

    Plasmid Kit
    #1000000054
    Purpose
    A collection that combines Golden Gate with Multisite Gateway cloning for assembly of complex fusion proteins or transgenesis constructs.
    Depositor
    Joachim Wittbrodt
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression, Eukaryotic Expression, Mammalian Expression, Zebrafish Expression
    Cloning Type
    Golden Gate
    Available Since
    Sept. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. PUF Assembly Kit

    Plasmid Kit
    #1000000051
    Purpose
    Assembly of Pumilio and FBF (PUF) homology domain based RNA-binding proteins for recognition of RNA targets.
    Depositor
    Huimin Zhao
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    Bacterial Expression, Mammalian Expression
    Editing Type
    PUF
    Cloning Type
    Golden Gate
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. ScEnSor Kit

    Plasmid Kit
    #1000000215
    Purpose
    To investigate the intracellular environment of Saccharomyces cerevisiae with a variety of biosensors.
    Depositor
    Lisbeth Olsson
    Application
    Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Available Since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only

  21. 4G-Cloning Plasmid Kit

    Plasmid Kit
    #1000000252
    Purpose
    Use 4G (Golden-Gate-guided Gibson) cloning for rapid assembly of single- or multi-gene expression vectors for multi-subunit protein complexes in various expression hosts.
    Depositor
    Stephan Gruber
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only

  22. Wnt Open Source

    Plasmid Kit
    #1000000022
    Purpose
    Plasmids containing ORFs of every human Wnt ligand, including modified versions that enable epitope tagging.
    Depositor
    Xi He, Marian Waterman
    Application
    Gene Expression and Labeling
    Vector Type
    Mammalian Expression
    Available Since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  23. EcoFlex MoClo Extension, Volume I

    Plasmid Kit
    #1000000253
    Purpose
    Plasmids to quickly and efficiently build a wide variety of gene expression constructs using the EcoFlex-standard MoClo system (Addgene #1000000080).
    Depositor
    Mikhail Shapiro
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only

  24. FNL Combinatorial Cloning Platform

    Plasmid Kit
    #1000000211
    Purpose
    A Frederick National Laboratory (FNL) combinatorial cloning set to generate many tagged expression clones in parallel for protein production, in vivo gene expression, and shRNA and CRISPR delivery.
    Depositor
    Dominic Esposito
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Bacterial Expression, Insect Expression, Mammalian Expression
    Cloning Type
    MultiSite Gateway
    Available Since
    Dec. 22, 2022
    Availability
    Academic Institutions and Nonprofits only

  25. Cancer Pathways ORFs Kit

    Plasmid Kit
    #1000000072
    Purpose
    Vectors containing wild-type and mutant ORFs for studying and screening oncogenic signaling pathways.
    Depositor
    David Sabatini, Kris Wood
    Application
    Gene Expression and Labeling
    Vector Type
    Lentiviral, Mammalian Expression
    Available Since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only

  26. SapTrap-SEC kit for C. elegans genome engineering

    Plasmid Kit
    #1000000109
    Purpose
    A collection of C. elegans plasmids used for building homologous repair templates that incorporate a self-excising drug selection cassette (SEC), based on the SapTrap CRISPR/Cas Toolkit.
    Depositor
    Bob Goldstein
    Application
    Genome Editing
    Vector Type
    Worm Expression
    Editing Type
    CRISPR
    Available Since
    June 23, 2017
    Availability
    Academic Institutions and Nonprofits only

  27. C. elegans miniMos transgenesis kit

    Plasmid Kit
    #1000000031
    Purpose
    Transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon.
    Depositor
    Erik Jorgensen
    Application
    Genome Editing
    Vector Type
    Worm Expression
    Editing Type
    Transposon
    Available Since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only

  28. Shen Lab DdCBE assembly kit

    Plasmid Kit
    #1000000212
    Purpose
    Generate DddA-derived cytosine base editors (DdCBEs).
    Depositor
    Bin Shen
    Application
    Genome Editing
    Vector Type
    Zebrafish Expression
    Editing Type
    Base Editing
    Available Since
    March 14, 2023
    Availability
    Academic Institutions and Nonprofits only

  29. MoBiFC

    Plasmid Kit
    #1000000222
    Purpose
    Modular bimolecular fluorescence complementation (MoBiFC) toolkit for analyzing protein-protein interactions in plants.
    Depositor
    Benjamin Field
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Plant Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  30. Zinc Finger Consortium Expression Vector and Modular Assembly Kit

    Plasmid Kit
    #1000000005
    Purpose
    Plasmids and strain to assemble Zinc finger nucleases (ZFNs) for performing genome engineering in a variety of organisms.
    Depositor
    Keith Joung
    Application
    Genome Editing
    Vector Type
    Bacterial Expression, Insect Expression, Mammalian Expression, Plant Expression
    Editing Type
    ZFN
    Available Since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  31. High Complexity Golden Gate Assembly Standards Kit (24 fragment lac cassette)

    Plasmid Kit
    #1000000221
    Purpose
    End-joining ligation fidelity and bias data predicts high accuracy junction sets for Golden Gate assembly, providing a positive control for complex assembly via blue/white screening in E. coli.
    Depositor
    Eric Cantor
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate
    Available Since
    June 1, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits

  32. Joung Lab REAL Assembly TALEN Kit

    Plasmid Kit
    #1000000017
    Purpose
    Restriction Enzyme And Ligation (REAL) TALEN assembly of TALE repeat arrays. Includes vector backbones for mammalian expression or in vitro transcription to produce TALE nuclease mRNAs.
    Depositor
    Keith Joung
    Application
    Genome Editing
    Vector Type
    In Vitro Transcription, Mammalian Expression
    Editing Type
    TALEN
    Available Since
    Nov. 4, 2011
    Availability
    Academic Institutions and Nonprofits only

  33. FX cloning Kit

    Plasmid Kit
    #1000000039
    Purpose
    Use fragment exchange (FX) cloning to generate constructs for screening protein expression in several organisms with a variety of N- and C-terminal tags.
    Depositor
    Eric Geertsma
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Bacterial Expression, Insect Expression, Mammalian Expression, Xenopus Expression, Yeast Expression
    Cloning Type
    FX Cloning
    Available Since
    April 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  34. Yamamoto Lab TALEN Accessory Pack

    Plasmid Kit
    #1000000030
    Purpose
    Supplemental kit for #1000000024 includes modified pFUS vectors to simplify Golden Gate assembly and mammalian expression/mRNA synthesis vectors containing unique shorter N- and C-terminal domains.
    Depositor
    Takashi Yamamoto
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector Type
    In Vitro Transcription, Mammalian Expression
    Editing Type
    TALEN
    Cloning Type
    Golden Gate
    Available Since
    March 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  35. Zinc Finger Consortium Expression Vector Kit v1.0

    Plasmid Kit
    #1000000010
    Purpose
    Plasmids and strain for the bacterial two-hybrid system used to screen Zinc finger nucleases (ZFNs).
    Depositor
    Keith Joung
    Application
    Genome Editing
    Vector Type
    Bacterial Expression, Insect Expression, Mammalian Expression, Plant Expression
    Editing Type
    ZFN
    Available Since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  36. The Expanded EasyClone-MarkerFree toolkit

    Plasmid Kit
    #1000000190
    Purpose
    Expansion of the EasyClone-MarkerFree Vector Set (#1000000098) with eight additional chromosomal loci for the stable integration of heterologous DNA into Saccharomyces cerevisiae by CRISPR/Cas9.
    Depositor
    Irina Borodina
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    USER
    Available Since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only

  37. Yeast GoldenBraid Cloning System and Toolkit

    Plasmid Kit
    #1000000138
    Purpose
    This toolkit allows generation of transcriptional units using GoldenBraid for integration in the S. cerevisiae genome at loci, YPRCΔ15 and YORWΔ22, to support high and stable transgene expression.
    Depositor
    Elena Caro Bernat
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (GoldenBraid)
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  38. LIC TAL Effector Assembly Kit

    Plasmid Kit
    #1000000023
    Purpose
    A TALE repeat unit library of 2-mer fragments that can be assembled via Ligation-independent cloning (LIC) into mammalian expression-ready TALEN constructs in 2 hierarchical assembly steps.
    Depositor
    Veit Hornung
    Application
    Genome Editing
    Vector Type
    Mammalian Expression
    Editing Type
    TALEN
    Available Since
    Jan. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  39. POMBOX MoClo YTK

    Plasmid Kit
    #1000000251
    Purpose
    Seamless and efficient assembly of single gene and multigene plasmids for genome integration in Schizosaccharomyces pombe.
    Depositor
    Tomáš Pluskal
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only

  40. Hamdoun lab FP fusion plasmids

    Plasmid Kit
    #1000000033
    Purpose
    Generate N- and C-terminal fusions to mCherry, Cerulean, mCitrine, or EGFP for screening fluorescent protein fusion expression in organisms or cells including Zebrafish, Xenopus, and C. elegans.
    Depositor
    Amro Hamdoun
    Application
    Gene Expression and Labeling
    Vector Type
    Worm Expression, Xenopus Expression, Zebrafish Expression
    Available Since
    July 24, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 81 - 120 of 166 results