We narrowed to 11 results for: his-tag

1. CRISPaint Gene Tagging Kit

   Plasmid Kit

   #1000000086

   Purpose

   Modular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tags

   Depositor

   Veit Hornung

   Available since

   Sept. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pMVP and pMAGIC Cloning Systems

   Plasmid Kit

   #1000000155

   Purpose

   pMVP and pMAGIC are cloning systems for adenoviral, lentiviral, expression, PiggyBac transposon, and Sleeping Beauty transposon vectors for transgene or RNAi delivery and dCas9-based engineering.

   Depositor

   Christopher Newgard

   Available since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

3. Yeast Secrete and Detect

   Plasmid Kit

   #1000000166

   Purpose

   Yeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.

   Depositor

   Sylvestre Marillonnet

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

4. Human Kinase Domain Constructs for Automated Bacterial Expression

   Plasmid Kit

   #1000000094

   Purpose

   68 His-tagged human kinase catalytic domain constructs and the respective phosphatases that enhance their bacterial expression; optimized for automated bacterial expression

   Depositor

   Nicholas Levinson, Markus Seeliger, John Chodera

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. AID* Kit

   Plasmid Kit

   #1000000120

   Purpose

   Expanded tool kit for the auxin-inducible degron (AID) system in yeast ( S. cerevisiae). Includes tags for easy detection by antibodies/microscopy and a set of selection markers.

   Depositor

   Helle Ulrich

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

6. FNL Combinatorial Cloning Platform

   Plasmid Kit

   #1000000211

   Purpose

   A collection of Multisite Gateway Entry clone plasmids which can be used in a combinatorial fashion to generate large numbers of tagged expression clones for protein expression in a variety of systems

   Depositor

   Dominic Esposito

   Available since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

7. Target Accelerator Pan-Cancer Mutant Collection

   Plasmid Kit

   #1000000103

   Purpose

   Panel of cancer alleles that have undergone basic, functional screening; full-length, human clones (454 mutant and 177 corresponding wild-type) in the Gateway cloning system

   Depositor

   Jesse Boehm

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

8. EcoFlex MoClo Extension, Volume I

   Plasmid Kit

   #1000000253

   Purpose

   Use the EcoFlex extension kit (volume one) to quickly and efficiently build a wide variety of gene expression constructs using the EcoFlex-standard MoClo system.

   Depositor

   Mikhail Shapiro

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

9. EcoFlex MoClo Kit

   Plasmid Kit

   #1000000080

   Purpose

   Golden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit design

   Depositor

   Paul Freemont

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

10. The Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit

    Plasmid Kit

    #1000000210

    Purpose

    Toolkit contains Cas9-gRNA vectors, integration plasmids, and organellar localization markers as a set of plasmids for Cas9-mediated integration of tagged genes and markers into the yeast genome.

    Depositor

    Vincent Martin

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

11. MoClo Plant Parts III: Transformation & Genome Engineering Kit

    Plasmid Kit

    #1000000236

    Purpose

    The MoClo Plant Parts III: Transformation & Genome Engineering Kit contains 94 Level 0 parts, compatible with the Phytobrick/MoClo system for golden gate assembly of plant transformation constructs.

    Depositor

    Daniel Voytas

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only