We narrowed to 9,886 results for: his-tag

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SPYtag-Histag-Avidin

Plasmid

#214836

Purpose

This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Article

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

SPYtag-Histag-Avidin

Expression

Bacterial

Promoter

T7

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-His-tag-HIS3MX6

Plasmid

#20762

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available Since

March 27, 2009

Availability

Academic Institutions and Nonprofits only

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His10-tag

Plasmid

#55179

Purpose

10xHis-tag

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

His10

Use

Synthetic Biology; Bacillus biobrick box

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSATAG

Plasmid

#97004

Purpose

Expressing MRSA TAG protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

DNA-3-methyladenine glycosylase I

Tags

N-ter TEV protease cleavable 6His

Promoter

T7

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD-6xHis-AviTag-ALFAtag-eGFP

Plasmid

#195854

Purpose

Bacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

6xHis-AviTag-ALFAtag-eGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET24-His6-Hatag-mCherry-ALFAtag

Plasmid

#253055

Purpose

Encodes His6 HA mCherry ALFA-tag in pET24 plasmid for bacterial expression with a T7 promoter

Depositor

Paul Young

Article

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

mCherry

Expression

Bacterial

Available Since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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pETDuet-1∆HisTag_hNMT_Nef

Plasmid

#66078

Purpose

Single vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli system

Depositor

Dieter Willbold

Article

Gluck et al PLoS One. 2010 Apr 9;5(4):e10081. doi: 1

Inserts

NMT (NMT1 Human)
Nef

Expression

Bacterial

Mutation

Deletion of first 80 amino acids

Promoter

T7-lacO

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pEXP5-aHL_spytag_his

Plasmid

#107334

Purpose

Monomeric SpyTag-6×-His alpha-hemolysin

Depositor

George Church

Article

Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

alpha-hemolysin-SpyTag-6xHis

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBacDual with PA+Histag

Plasmid

#84870

Purpose

Baculovirus Expression of Bacillus anthracis protective antigen

Depositor

Russell Vance

Article

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Insert

PA

Use

Baculovirus

Tags

His

Expression

Insect

Promoter

Polyhedrin

Available Since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pTrcHIS-7D12Gln13TAG

Plasmid

#125264

Purpose

Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzF

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

Gln13TAG 7D12

Tags

His-Tag

Expression

Bacterial

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pETDuet-1∆HisTag_Nef_MCS2

Plasmid

#66079

Purpose

Single vector system expressing NEF allowing myristoylation of recombinant protein produce in Ecoli system

Depositor

Dieter Willbold

Article

Gluck et al PLoS One. 2010 Apr 9;5(4):e10081. doi: 1

Insert

Nef

Tags

6xHis and HRV 3C Site

Expression

Bacterial

Promoter

T7-lacO

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only
pJUMP18-Histag_C

Plasmid

#127035

Purpose

Basic Part C- C-terminus; polyhistidine tag

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part His6_C

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTrcHis-7D12CTermTAG

Plasmid

#125267

Purpose

Expresses 7D12 anti-EGFR nanobodies with UAG codon at c-terminus for synthetic amino acid in combination with pEVOL-pAzF

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

CTermTAG

Tags

His-Tag

Promoter

Trc

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pTrcHis-7D12Gly42TAG

Plasmid

#125265

Purpose

Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gly42 for synthetic amino acid in combination with pEVOL-pAzF

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

7D12 Gly42 nanobody

Tags

His-tag

Expression

Bacterial

Promoter

trc promoter

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-Histag_N

Plasmid

#127033

Purpose

Basic Part N- N-terminus; polyhistidine tag

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part His6_N

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTrcHis-7D12Asp73TAG

Plasmid

#125266

Purpose

Expresses 7D12 anti-EGFR nanobodies with UAG codon at Asp73 for synthetic amino acid in combination with pEVOL-pAzF

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

7D12 Asp73TAG

Tags

His-Tag

Expression

Bacterial

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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AviTag-His6-OAZ-SPM(FrpA)-Ctag

Plasmid

#246646

Purpose

Expresses AviTag-His6-OAZ-SPM(FrpA)-Ctag in bacterial cells for calcium-induced NeissLock protein ligation

Depositor

Mark Howarth

Article

Lim et al ACS Chem Biol. 2025 Sep 15. doi: 10.1021

Insert

AviTag-His6-OAZ-SPM(FrpA)-Ctag

Tags

AviTag, Ctag, and His-tag

Expression

Bacterial

Mutation

Residues 95-219 of human Orinithine Decarboxylase…

Promoter

T7

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pET29b_SpyTag003-L-VHH21-His

Plasmid

#233333

Purpose

To express nanobody VHH21 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-VHH21-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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6xHis-TagRFP-T_A1aY1

Plasmid

#158754

Purpose

Expression of 6x His tagged TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

6xHistidine_TagRFP-T_A1aY1

Tags

6x-Histidine tag and TagRFP-T

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pQE80L Hu IL-15-His tag

Plasmid

#183256

Purpose

Bacterial expression of human IL-15 with N-terminal His tag

Depositor

Alexander McLellan

Insert

IL-15 (IL15 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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