27,466 results
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Plasmid#227274PurposeAAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AMPK-SPARK
Plasmid#226255Purposephases-of-separation based activity reporter of AMPKDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAMPK-SPARK
ExpressionMammalianAvailable sinceOct. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
P4-TadCBEd
Plasmid#225143PurposePackaging plasmid for TadCBEd in v3b PE-eVLP architectureDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertP4-TadCBEd
ExpressionMammalianAvailable sinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1
Plasmid#222994PurposeMammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1
UseCRISPRTagsPuroExpressionMammalianPromoterpCAGAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpCas9(H840A)-T2A-EGFP
Plasmid#221231PurposeExpress nCas9 nickase (H840A) with GFP reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9(H840A)-T2A-EGFP
ExpressionMammalianMutationH840AAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-Nir1-LNS2
Plasmid#220084Purposehigh affinity PA biosensor with red fluorophore (also known as mCherry-PILS-Nir1)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPITPNM3 (PITPNM3 Human)
TagsmCherry-GGSGGMExpressionMammalianMutationamino acids 613-897PromoterCMVAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
eGFP-SUN1
Plasmid#213515PurposeExpresses human eGFP-SUN1 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSUN1 (SUN1 Human)
ExpressionMammalianMutationSUN1 has a silent mutation on its gene (nucleotid…PromoterCMVAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDualIN-SMN2
Plasmid#214430PurposeDual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSMN2 EXON 7 (SMN2 Human)
ExpressionMammalianAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-GR
Plasmid#215094PurposeExpresses HA-tagged Glucocorticoid Receptor in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNR3C1 (NR3C1 Human)
TagsHAExpressionMammalianPromoterCMVAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
RDX-mCherry
Plasmid#214476PurposePlasmid for transient transfection of RDX-mCherry fusion protein in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRDX (RDX Human)
TagsmCherryExpressionMammalianMutationSequence matches cDNA clone MGC:48283 IMAGE:52844…PromoterCMVAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB-mTSPAN4-mCherry
Plasmid#210330PurposeOverexpression of mTSPAN4DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmTSPAN4 (Tspan4 Mouse)
TagsmCherryExpressionMammalianAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
ARTi
Plasmid#214064PurposeFluorescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTurboRFP
ExpressionMammalianMutationLinker sequence present between RFP and GFPAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
nLbCas12a
Plasmid#213745PurposeFor circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnLbCas12a
UseCRISPRTagsBPNLSExpressionMammalianMutationD156R, R1138APromoterCMVAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCer-DHX9
Plasmid#211408Purposemammalian expression of human DHX9 N-terminally fused to mCeruleanDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDHX9 (DHX9 Human)
TagsmCeruleanExpressionMammalianAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
B-GRAM-W
Plasmid#211707PurposeExpression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertB-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
TagsddFP-B3ExpressionMammalianMutationChanged Glycine 187 to TryptophanPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU6-PspCas13b-GEMSgRNA2
Plasmid#204768PurposepU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertguide RNA targeting GEMS m6A sensor mRNA
UseCRISPRExpressionMammalianPromoterU6Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hALDOA
Plasmid#210809PurposeFor transient transfectionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertALDOA (ALDOA Human)
TagsHAExpressionMammalianPromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-SpG-P2A-hMLH1dn
Plasmid#211550PurposePrime editing in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPEmax-SpG-P2A-hMLH1dn
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Kif2c
Plasmid#208651PurposeMammalian expression plasmid expressing eGFP-Kif2c driven by CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKif2c (KIF2C Human)
TagseGFPExpressionMammalianAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lyso-GA
Plasmid#209874PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the lysosomal membrane with human LAMP1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertddGFP A
TagsLAMP1ExpressionMammalianPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only