Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

27,006 results

Showing: 7281 - 7300 of 27006 results

  1. pICE-HA-MRE11-H63D

    Plasmid
    #82036
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of H63D exonuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Publication
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MRE11 (MRE11 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Exonuclease-dead mutant: H63D MRE11
    Promoter
    CMV-tet
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    82036-plasmid-map-sequence-id-155389

    Enlarge

  2. pMA-SpCas9-g4

    Plasmid
    #80787
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80787-plasmid-map-sequence-id-162407

    Enlarge

  5. mbYFPQS

    Plasmid
    #80742
    Purpose
    membrane-targeted biosensor for monitoring changes in intracellular chloride
    Depositor
    Susan Amara, Susan Ingram
    Publication
    Watts et al PLoS One. 2012;7(4):e35373. doi: 10.1371
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YFPQS
    Tags
    palmitoylation signal sequence of neuromodulin
    Expression
    Mammalian
    Mutation
    H148Q (CAC to CAG) and V163S (GTG to AGC).
    Promoter
    CMV
    Available since
    Aug. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles

  7. pEx1-pEF-H2B-mCherry-T2A-rTetR-EED

    Plasmid
    #78101
    Purpose
    Expresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.
    Depositor
    Michael Elowitz
    Publication
    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2B-mCherry-T2A-rTetR-EED (EED Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    rTetR
    Expression
    Mammalian
    Promoter
    pEF alpha
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    78101-plasmid-map-sequence-id-146271

    Enlarge

  11. pCS2+MCS-P2A-sfGFP

    Plasmid
    #74668
    Purpose
    Plasmid for expression of wild-type or mutant cDNA fragments (mutation reporter) or complete cDNAs in frame with superfolderGFP.
    Depositor
    Jason Berman
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sfGFP
    Expression
    Mammalian
    Available since
    May 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    74668-plasmid-map-sequence-id-146800

    Enlarge

  12. pcDNA3-NP

    Plasmid
    #74737
    Purpose
    mammalian expression of the Nucleoprotein of the A/New Caledonia/20/99 (H1N1) Influenza virus
    Depositor
    Elisa Vicenzi
    Publication
    Di Pietro et al J Virol. 2013 Apr;87(8):4523-33. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nucleoprotein
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    74737-plasmid-map-sequence-id-336041

    Enlarge

  13. Rlucrarr3-Flash1

    Plasmid
    #74129
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of Arrestin3.
    Depositor
    Louis Luttrell
    Publication
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Arrestin3
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  14. pEYFP-hDF

    Plasmid
    #72584
    Purpose
    human dysferlin full length tagged with YFP
    Depositor
    Renzhi Han
    Publication
    Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dysferlin (DYSF Human)
    Tags
    EYFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    72584-plasmid-map-sequence-id-134969

    Enlarge

  16. pSG5-BRLF1

    Plasmid
    #72635
    Purpose
    Mammalian expression vector for Epstein-Barr virus Rta immediate-early replication and transcription activator
    Depositor
    S. Diane Hayward
    Publication
    Sarisky et al J Virol. 1996 Dec;70(12):8340-7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BRLF1
    Expression
    Mammalian
    Available since
    Feb. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  18. FRB-ECFP(W66A)-Giantin

    Plasmid
    #67903
    Purpose
    Expresses a Golgi located FRB domain in mammalian cells (the ECFP is not fluorescent)
    Depositor
    Dorus Gadella
    Publication
    van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FRB-ECFP(W66A)-Giantin
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    67903-plasmid-map-sequence-id-118088

    Enlarge

Showing: 7281 - 7300 of 27006 results