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27,006 results

Showing: 8081 - 8100 of 27006 results

  2. pcDNA3-ExRai-AktAR(T/A)

    Plasmid
    #118463
    Purpose
    Negative-control mutant for ExRai-AktAR biosensor.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ExRai-AktAR(T/A)
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Mutation
    Contains Thr-to-Ala mutation in substrate sequenc…
    Promoter
    CMV
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    118463-plasmid-map-sequence-id-230224

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  3. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q54

    Plasmid
    #111727
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Publication
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q54 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    111727-plasmid-map-sequence-id-272590

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  4. H2B-mGarnet2

    Plasmid
    #104312
    Purpose
    codes for a fusion of fluorescent protein mGarnet2 and human histon 2B (H2B), makes the H2B visible in live cells
    Depositor
    Ulrich Nienhaus
    Publication
    Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2B-mGArnet2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    104312-plasmid-map-sequence-id-225638

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  5. pPtGE35

    Plasmid
    #107999
    Purpose
    Expresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation
    Depositor
    David Edgell
    Publication
    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    I-TevI nuclease and partial linker domain
    Use
    CRISPR and Synthetic Biology; Episomal vector for…
    Tags
    Cas9
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    107999-plasmid-map-sequence-id-217151

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  6. AICSDP-34: TNNI1-mEGFP

    Plasmid
    #114411
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Publication
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TNNI1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114411-plasmid-map-sequence-id-220877

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  7. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q42

    Plasmid
    #111746
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Publication
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q42 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111746-plasmid-map-sequence-id-220484

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  8. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q19

    Plasmid
    #111733
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Publication
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q19 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111733-plasmid-map-sequence-id-304484

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  9. pDEST-FRT/TO-eGFP-REV7

    Plasmid
    #114128
    Purpose
    Expresses N-terminally tagged eGFP-REV7 in mammalian cells
    Depositor
    Daniel Durocher
    Publication
    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    REV7 (MAD2L2 Human)
    Use
    Genomic integration, tetracycline inducible
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    CMV/TetO2
    Available since
    Aug. 15, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    114128-plasmid-map-sequence-id-218413

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  11. PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid
    #102885
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DDdCas9VP192-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    102885-plasmid-map-sequence-id-259794

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  12. PB-tight-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid
    #102889
    Purpose
    PiggyBac transposon system construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DDdCas9VP192-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    TRE-tight
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    102889-plasmid-map-sequence-id-198247

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  13. A3Ai-Cas9n-UGI-NLS

    Plasmid
    #109425
    Purpose
    Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Publication
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    109425-plasmid-map-sequence-id-212947

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  16. pcDNA3-Flag-Tet2 CD

    Plasmid
    #72219
    Purpose
    Express flag-tagged mouse Tet2 catalytic domain.
    Depositor
    Yi Zhang
    Publication
    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ten-Eleven Translocation 2 (Tet2 Mouse)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    The plasmid expresses TET2 catalytic domain.
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
Showing: 8081 - 8100 of 27006 results