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Reb_83 aka PAS624

Plasmid

#71229

Purpose

Reb_1 with RebA(G53A, D89G)

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebA(G53A, D89G)

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
pFS_0143-pET30-Fusicatenibacter_saccharivorans

Plasmid

#116952

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with classic acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pTKEI-tLOV

Plasmid

#100574

Purpose

Expression plasmid for the thermostable anaerobic fluorescent protein tLOV

Depositor

David Savage

Article

Higgins et al ACS Synth Biol. 2017 Jul 24. doi: 10.102

Insert

tLOV

Tags

6xHis

Expression

Bacterial

Promoter

pTrc

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-neoCV

Plasmid

#73889

Purpose

fission yeast drug selection marker

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Insert

neoCV

Expression

Bacterial, Mammalian, and Y…

Promoter

CMV

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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M26I DJ-1 pET15b

Plasmid

#60677

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

Insert

M26I DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26I

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
T7 promoter + rpsL reporter plasmid

Plasmid

#156455

Purpose

A mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Inserts

rpsL
TcR

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

tac and tet

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON

Plasmid

#208797

Purpose

Xenopus laevis His6-Myc-DONSON wild type

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Tags

His and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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GST-FUS 266-526

Plasmid

#44981

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS (266-526aa) (FUS Human)

Tags

GST

Expression

Bacterial

Mutation

contains amino acids 266-526

Promoter

tac

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only
pBad-HisB-GGvT

Plasmid

#87362

Purpose

Expresses GGvT, a tandem homodimer green fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

GGvT

Tags

His-Tag

Expression

Bacterial

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pB1H2w5-12-C81S

Plasmid

#18043

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

HhaI C81S mutant

Expression

Bacterial

Mutation

Simply used as an insert between Kpn1 and Xba1

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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