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32,870 results

Showing: 3281 - 3300 of 32870 results

  3. pHis17_ParM _2

    Plasmid
    #78202
    Purpose
    Expression of double-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor
    Depositor
    Martin Webb
    Article
    Kunzelmann et al ACS Chem Biol. 2010 Apr 16;5(4):415-25.
    Insert
    parM (K33A D63C T174A T175N D224C C287A)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    K33A D63C T174A T175N D224C C287A
    Promoter
    unknown
    Available Since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    78202-plasmid-map-sequence-id-146031

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  4. pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]

    Plasmid
    #74102
    Purpose
    plasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)
    Depositor
    Ben Szaro
    Article
    Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:
    Inserts
    Xenopus laevis beta-tubulin gene promoter region and 5'UTR
    Green Lantern Green Fluorescent Protein
    Rabbit beta 1-globin gene 3'UTR
    Expression
    Bacterial
    Available Since
    May 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74102-plasmid-map-sequence-id-148113

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  10. pET28a-HsSENP2

    Plasmid
    #71466
    Purpose
    Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 2 (SENP2 Human)
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available Since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pST50Trc1-HISNDHFR

    Plasmid
    #63940
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis; TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63940-plasmid-map-sequence-id-511340

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  15. pComb3HTT

    Plasmid
    #63900
    Purpose
    Backbone of pComb3H containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control.
    Depositor
    Carlos Barbas
    Article
    Barbas et al Methods, A companion to Methods in Enzym
    Insert
    TT Fab
    Use
    Phage display
    Tags
    geneIII
    Expression
    Bacterial
    Promoter
    lacZ
    Available Since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63900-plasmid-map-sequence-id-119142

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  17. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

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  19. pBAD18-Gm

    Plasmid
    #65100
    Purpose
    bacterial expression vector with Gentamycin selection, based on pBAD18
    Depositor
    Juan Tomas
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    June 5, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 3281 - 3300 of 32870 results