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31,793 results

Showing: 3381 - 3400 of 31793 results

  8. pMT-sox10-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80063
    Purpose
    Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Sox10 promoter
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80063-plasmid-map-sequence-id-417621

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  9. pCm C-term MBP NikJC199U

    Plasmid
    #174365
    Purpose
    Expression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.
    Depositor
    Brandon Greene
    Article
    Caceres et al Methods Enzymol. 2022;662:119-141. doi:
    Insert
    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    Tags
    8xHis, MBP, and TEV
    Expression
    Bacterial
    Mutation
    C199U
    Promoter
    T7
    Available Since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174365-plasmid-map-sequence-id-342848

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  10. pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher

    Plasmid
    #117656
    Purpose
    Bacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.
    Depositor
    Bo Huang
    Article
    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.
    Inserts
    sfCherry2(1-10)
    sfCherry2(11)-SpyCatcher002
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    117656-plasmid-map-sequence-id-226764

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  11. pBJ6

    Plasmid
    #167135
    Purpose
    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter
    Depositor
    Akira Mine
    Article
    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d
    Insert
    luxCDABE
    Use
    Luciferase
    Expression
    Bacterial
    Promoter
    Kanamycin promoter
    Available Since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167135-plasmid-map-sequence-id-330485

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  13. pcDNA3.1.Gαi1-LgB91

    Plasmid
    #134342
    Purpose
    Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαi1-LgB91 (GNAI1 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134342-plasmid-map-sequence-id-306400

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  14. KARS_pNIC-Bio3

    Plasmid
    #153051
    Purpose
    Expression construct for producing Avi tagged Lysyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    KARS (KARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153051-plasmid-map-sequence-id-304466

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  17. pET28-H12TEV

    Plasmid
    #208635
    Purpose
    for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    6xhis
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208635-plasmid-map-sequence-id-433640

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  18. pDsEndoNA2

    Plasmid
    #174728
    Purpose
    Expresses DsRed-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.
    Depositor
    Jukka Finne
    Article
    Lehti et al Nat Commun. 2017 Dec 4;8(1):1915. doi: 1
    Insert
    DsRed-Monomer
    Tags
    Histag
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    174728-plasmid-map-sequence-id-343513

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Showing: 3381 - 3400 of 31793 results