30,424 results

1. pET28a_Pro-IL-33

   Plasmid

   #214342

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL33 (IL33 Human)
   

   Use

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJJS002

   Plasmid

   #188325

   Purpose

   PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.

   Depositor

   Mike Boxem

   Article

   Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

   Insert

   Linker::GFP::mIAA7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQIq_MRGS_HIS8_(27F3)_FLAG

   Plasmid

   #199615

   Purpose

   Encodes DARPin-FLAG 27F3 for bacterial expression and column purification

   Depositor

   Shiva Tyagarajan

   Article

   Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

   Insert

   DARPin-FLAG 27F3
   

   Use

   Tags

   8xHis and FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHH0103_PFN1_Profilin

   Plasmid

   #110028

   Purpose

   Protein expression and purification of PFN1_Profilin

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   PFN1_Profilin (PFN1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMLRT

   Plasmid

   #78234

   Purpose

   Expresses Murine Leukemia Reverse Transcriptase (MLRT)

   Depositor

   Christopher Voigt

   Article

   Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10

   Insert

   Murine leukemia reverse transcriptase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23102

   Available Since

   Aug. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE-60 Grx1-roGFP2-His

   Plasmid

   #64799

   Purpose

   Bacterial expression of His-tagged Grx1-roGFP2

   Depositor

   Tobias Dick

   Article

   Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Tags

   His and roGFP2

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDB179-DepoPETase

   Plasmid

   #218702

   Purpose

   Encodes codon optimized DepoPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   DepoPETase
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTHSSe_49

   Plasmid

   #109247

   Purpose

   pSC101 var7 BbsI cloning vector

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23105

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-His6-SUMO-MproC145A

   Plasmid

   #172653

   Purpose

   Bacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMO

   Depositor

   Stephen Harrison

   Article

   MacDonald et al ACS Infect Dis. 2021 Sep 10;7(9):2591-25

   Insert

   SARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   6xHis Tag and SUMO

   Expression

   Bacterial

   Mutation

   Catalytic Cysteine 145 mutated to Alanine

   Promoter

   T7

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC_pThrRS_SeptRNAv2.0CUA_OXB20-PduX_EFSep

    Plasmid

    #173899

    Purpose

    The pThr/tRNAv2.0CUA pair enables the biosynthesis of proteins containing pThr

    Depositor

    Jason W Chin

    Article

    Zhang et al Nat Methods. 2017 Jul;14(7):729-736. doi

    Inserts

    ThrRS(v1.0)
    tRNA(v2.0)CUA
    pduX
    EF-Sep
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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