30,424 results

1. IMS-APEX2

   Plasmid

   #79058

   Purpose

   Expresses APEX in the mitochondrial intermembrane space.

   Depositor

   Alice Ting

   Article

   Hung et al Mol Cell. 2014 Jul 17;55(2):332-41. doi:

   Insert

   APEX
   

   Use

   Tags

   V5

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7 promoter

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCK005.6

   Plasmid

   #153025

   Purpose

   dCas9, MCP-SoxS(R93A/S101A), J106 scRNA

   Depositor

   Jesse Zalatan

   Article

   Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

   Insert

   dCas9, MCP-SoxS(R93A/S101A), J106 scRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   SoxS has R93A and S101A mutations

   Promoter

   Available Since

   Aug. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJCSUP35(1-253)

   Plasmid

   #1089

   Depositor

   Susan Lindquist

   Article

   Glover et al Cell 1997 May 30;89(5):811-9.

   Insert

   SUP35 (SUP35 Budding Yeast)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   amino acids 1 - 253 of SUP35

   Promoter

   Available Since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pTriEx4-Cdc42-2G

   Plasmid

   #68814

   Purpose

   FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)

   Depositor

   Olivier Pertz

   Article

   Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

   Insert

   Cdc42 activation reporter
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. HLTV-hTRIM21

   Plasmid

   #104973

   Purpose

   Purification of His-Lipoyl-hTRIM21 protein from bacteria

   Depositor

   Leo James

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   Tripartite Motif containing 21 (TRIM21 Human)
   

   Use

   Tags

   His-Lipoyl

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pH6-MBP-TEV-BirA

   Plasmid

   #179694

   Purpose

   Expresses His6 tagged MBP-BirA fusion protein

   Depositor

   Mark Arbing

   Insert

   BirA
   

   Use

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. RRAS

   Plasmid

   #38815

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   RRAS (Rras Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pTET GFP10-FRB/FKBP-GFP11

   Plasmid

   #182497

   Purpose

   Tripartite split-GFP. E. coli vector. Inducible coexpression of GFP10-FRB and FKBP-GFP11 coiled coils

   Depositor

   Stéphanie Cabantous

   Article

   Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

   Inserts

   FRB
   FKBP (Fkbp1a Synthetic, Mouse)
   

   Use

   Tags

   split GFP10 tripartite and split GFP11 tripartite

   Expression

   Bacterial

   Mutation

   Promoter

   TET

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRE-Tn7-145

   Plasmid

   #118561

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mScarlet-I
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJYS3_ΔcrtYf

    Plasmid

    #85542

    Purpose

    Constitutive transcription of FnCpf1 and crRNA of crtYf

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    crRNA of crtYf
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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