32,529 results
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Plasmid#38872PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only
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3xFLAG-dCas9/p-bacteria
Plasmid#64325PurposeExpresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR; Tetracycline-inducibleTags3xFLAGExpressionBacterialMutationD10A, H840APromoterpLtetO-1Available SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRESCA-syntide
Plasmid#132415PurposeFRET-based sensor for CaMKII activityDepositorInsertFRESCA-syntide
ExpressionBacterial and MammalianPromoterCMVAvailable SinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ydf NanoLuc
Plasmid#225656PurposeReporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.DepositorInsertNanoLuciferase
UseCell free expressionTagsMycExpressionBacterialPromoterPydfJ115Available SinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarX Myc
Plasmid#225651PurposeExpresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.DepositorAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human_TFAM_NoMTS_pET28
Plasmid#34705DepositorInsertTFAM (TFAM Human)
Tags6XHisExpressionBacterialMutationEncodes residues 43–246, corresponding to full-le…PromoterT7Available SinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-dCas9-VP64-6xHis
Plasmid#62935PurposeExpression of dCas9-VP64-6xHis in bacterial cellsDepositorInsertdCas9-VP64
Tags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A and H840A amino acid changes render Cas9 nuc…PromoterT7Available SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
Beta-Lactamase
Plasmid#62729PurposeExpresses Beta-Lactamase protein in bacterial cellsDepositorInsertBeta-Lactamase
TagsHis tagExpressionBacterialMutationDeletion of periplasmic signal domainPromoterT7Available SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_crGE
Plasmid#172927PurposeCrowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.DepositorInsertCrowdingSensorGE
TagsHisExpressionBacterialPromoterT7Available SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 NDM-1
Plasmid#112883PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only