30,481 results

1. SPLICSFAST-NU-MT-P2A

   Plasmid

   #214898

   Purpose

   Expresses equimolar Nucleus- and Mitochondria- targeted split-FAST fragments for Short-range NU-MT Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-NU-MT-P2A
   

   Use

   Tags

   none

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEVOL-pylT-AznLRS

   Plasmid

   #172482

   Purpose

   For E.Coli expression, 306L309L348I384F411W

   Depositor

   Wenshe Liu

   Article

   Wang et al Angew Chem Int Ed Engl. 2017 Feb 1;56(6)

   Insert

   AznLRS and pylTcua
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available Since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAD-LyseR

   Plasmid

   #99244

   Purpose

   For autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

   Depositor

   Jeff Hasty

   Article

   Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

   Insert

   phage lambda gene R (R phage lambda)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   beta-lactamase promoter

   Available Since

   Oct. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNBU2_repA2

   Plasmid

   #211911

   Purpose

   Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.

   Depositor

   Adam Arkin

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRelA*

   Plasmid

   #175590

   Purpose

   Tet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.

   Depositor

   Greg Bokinsky

   Article

   Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

   Insert

   Catalytic domain of E.coli relA gene.
   

   Use

   Tags

   Fused with a flexible GS linker to mVenus

   Expression

   Bacterial

   Mutation

   Only the catalytic domain of the enzyme is used (…

   Promoter

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MBP-CHC(1-1074)-His6

   Plasmid

   #100748

   Purpose

   Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   CLTC (CLTC Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   Residues 1-1074

   Promoter

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEM-Sox2

   Plasmid

   #16353

   Depositor

   James Thomson

   Article

   Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

   Insert

   Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCW-LIC-N8C_Fab3a

   Plasmid

   #203689

   Purpose

   Expresses N8C_Fab3a

   Depositor

   Brenda Schulman

   Article

   Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

   Insert

   N8C_Fab3a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Alpha-Synuclein-His

   Plasmid

   #186802

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses Alpha-Synuclein-His.

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   Alpha-Synuclein-His (SNCA Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETM-41-HsPABPC1_J

    Plasmid

    #146644

    Purpose

    Bacterial Expression of HsPABPc1

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

    Insert

    HsPABPc1 (PABPC1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge