30,513 results

1. pNBU2_repA2

   Plasmid

   #211911

   Purpose

   Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.

   Depositor

   Adam Arkin

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRE140

   Plasmid

   #118492

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   TagBFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)

   Plasmid

   #160540

   Purpose

   Expresses codon optimized SARS-CoV-2 NSP7, NSP8, and NSP12 in E. coli.

   Depositor

   Kenneth Johnson

   Article

   Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

   Inserts

   SARS-CoV-2 NSP7 (ORF1ab Synthetic)
   SARS-CoV-2 NSP8 (ORF1ab Synthetic)
   SARS-CoV-2 NSP12 (ORF1ab Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. MBP-CHC(1-1074)-His6

   Plasmid

   #100748

   Purpose

   Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   CLTC (CLTC Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   Residues 1-1074

   Promoter

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSFDuet-1_mNeongreen

   Plasmid

   #182413

   Purpose

   Bacterial expression of mNeongreen

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   mNeongreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAWP78-PVCpnf_pvc13-HA

   Plasmid

   #198296

   Purpose

   Produces PVCs retargeted with HA tag

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   PVCpnf_pvc13-HA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW-LIC-N8C_Fab3a

   Plasmid

   #203689

   Purpose

   Expresses N8C_Fab3a

   Depositor

   Brenda Schulman

   Article

   Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

   Insert

   N8C_Fab3a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM-41-HsPABPC1_J

   Plasmid

   #146644

   Purpose

   Bacterial Expression of HsPABPc1

   Depositor

   Elisa Izaurralde

   Article

   Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

   Insert

   HsPABPc1 (PABPC1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   two silent mutations compared to the sequence giv…

   Promoter

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTriEx4-Rac1-2G

   Plasmid

   #66110

   Purpose

   Second generation Rac1 FRET biosensor

   Depositor

   Olivier Pertz

   Article

   Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

   Insert

   Second generation Rac1 FRET biosensor
   

   Use

   Tags

   His-Myc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRE-Tn7-156

    Plasmid

    #118570

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mCardinal
    

    Use

    Minitn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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