29,771 results

1. pBAD/HisB-PAmCherry1

   Plasmid

   #31931

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

   Insert

   PAmCherry1
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

   Promoter

   araB

   Available since

   Sept. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEB1-SCFP3A

   Plasmid

   #103970

   Purpose

   Plasmid encoding SCFP3A

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   SCFP3A
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (F64L, S65T, …

   Promoter

   proC

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYTK076

   Plasmid

   #65183

   Purpose

   Encodes HIS3 as a Type 6 part to be used in the Dueber YTK system

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Insert

   HIS3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLT 653

   Plasmid

   #59996

   Purpose

   Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/klp-16 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny

   Depositor

   Lisa Timmons

   Article

   Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(

   Inserts

   dhc-3 (dhc-3 Nematode)
   klp-16 (klp-16 Nematode)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   partial genomic

   Promoter

   Available since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPSU50

   Plasmid

   #173832

   Purpose

   Expresses 50 kD Penn State ladder protein

   Depositor

   Song Tan

   Article

   Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

   Insert

   STRHSTPABMBP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-30 Ubwt (2)

   Plasmid

   #26685

   Depositor

   Linda Hicke

   Article

   French et al J Biol Chem. 2009 May 1. 284(18):12071-9

   Insert

   Ubiquitin
   

   Use

   Tags

   His and S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pMRE-Tn7-132

   Plasmid

   #118550

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sGFP2
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28b-HRASLS3-1-132

   Plasmid

   #100725

   Purpose

   Expression of N-terminal region of human HRASLS3 in E.coli

   Depositor

   Byung Il Lee

   Article

   Han et al Protein Expr Purif. 2010 May;71(1):103-7

   Insert

   HRASLS3 (PLAAT3 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET23b-Casp6-His

   Plasmid

   #11823

   Depositor

   Guy Salvesen

   Article

   Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

   Insert

   Caspase 6 (CASP6 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. Stat2 pBluescript SK (-)

    Plasmid

    #8705

    Depositor

    Jim Darnell

    Article

    Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

    Insert

    Stat2 (STAT2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFLAG-XBP1u-FLuc

    Plasmid

    #31239

    Depositor

    Jimtong Horng

    Article

    Jheng et al Cell Microbiol. 2010 Jun . 12(6):796-813

    Insert

    FLAG-XBP1u-FLuc (XBP1 Human)
    

    Use

    Tags

    Flag and Fluc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Aug. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pYUBDuet

    Plasmid

    #37278

    Depositor

    Ted Baker

    Article

    Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

    Type

    Empty backbone

    Use

    Mycobacteria shuttle vector

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETSXM2-pLacI-GFP

    Plasmid

    #174617

    Purpose

    Bacterial expression of GFP.

    Depositor

    I-Son Ng

    Article

    I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacI

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. human EB1 in PET28a for protein expression

    Plasmid

    #39297

    Depositor

    Jennifer Tirnauer, Tim Mitchison

    Article

    Tirnauer et al Mol Biol Cell. 2002 Oct . 13(10):3614-26

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pGEM cAgg (CT#689)

    Plasmid

    #13945

    Depositor

    Cliff Tabin

    Article

    Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

    Insert

    Aggrecan (ACAN Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nucleotides 113-800

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pET28_Caspase-4_K356D

    Plasmid

    #214312

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    K356D

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-SUMO-mGSDMD

    Plasmid

    #111560

    Purpose

    Express mouse GSDMD in E.coli

    Depositor

    Hongbo Luo

    Article

    Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Use

    Tags

    His, SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRT498-3xOtUBD

    Plasmid

    #190088

    Purpose

    Expresses 3 tandem OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

    Depositor

    Mark Hochstrasser

    Article

    Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

    Insert

    3xOtUBD
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTorPE-R-GECO1

    Plasmid

    #32465

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    R-GECO1.0
    

    Use

    Lab constructed

    Tags

    6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…

    Expression

    Bacterial

    Mutation

    Substitutions relative to the mApple-derived anal…

    Promoter

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPath-BGC08-SGE

    Plasmid

    #200006

    Purpose

    BGC08 pathway. SGE

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Pathways
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge