30,514 results

1. pMGE-T7

   Plasmid

   #153021

   Purpose

   Single-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.

   Depositor

   Vito Valiante

   Article

   Kufs et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MAPK1

   Plasmid

   #73248

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   MAPK1 (MAPK1 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBB550

   Plasmid

   #27396

   Depositor

   Bernd Bukau

   Article

   de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

   Insert

   dnaK, dnaJ, GroESL
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. His-drebrin

   Plasmid

   #40362

   Depositor

   Phillip Gordon-Weeks

   Article

   Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

   Insert

   drebrin E2 (DBN1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pUC18T-mini-Tn7T-Zeo-gfpmut3

   Plasmid

   #65037

   Purpose

   ZeoR mini-Tn7 vector, mobilizable, for GFP tagging bacteria

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Insert

   gfpmut3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. HotPETase_pET21b

   Plasmid

   #215407

   Purpose

   Expression construct for HotPETase gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   HotPETase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-28a(+)-FGF1-4X

   Plasmid

   #120282

   Purpose

   Produce human recombinant FGF1-4X in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   Basic fibroblast growth factor 1 mutant
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE30-hPolB

   Plasmid

   #70761

   Purpose

   bacterial expression of human DNA Polymerase beta (POLB)

   Depositor

   Primo Schaer

   Article

   Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

   Insert

   POLB (POLB Human)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   R333L

   Promoter

   T5/lac operon

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNP1723

   Plasmid

   #222727

   Purpose

   sfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Type

   Empty backbone

   Use

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE30-His-PheRS

    Plasmid

    #124116

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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