34,218 results
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Plasmid#79882PurposeUbiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
TagsAviExpressionBacterialPromoterUbiquitinAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-Avi-Cerulean-RanGAP
Plasmid#79878PurposeBeta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
TagsAviExpressionBacterialPromoterBeta-actin2Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSF-DUET Cas1 Cas2
Plasmid#80088PurposeEncodes Cas1 and Cas2DepositorPublicationAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1
Plasmid#81133Purposemammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domainDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsTagsmVenusExpressionBacterial, Insect, and Mamm…Available sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEN-L1-K2-L2
Plasmid#80684PurposeGateway Entry vector containing a temperature-sensitive mouse DHFRDepositorUseSynthetic BiologyTags3xHAExpressionBacterial, Insect, Mammalia…Mutationmouse DHFR with N-terminal UbiquitinAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCOLA-T7-YhjH
Plasmid#79165PurposeExpresses YhjH under a T7 promoterDepositorInsertyhjH
ExpressionBacterialPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET31b-T7-Spinach2-Ct
Plasmid#79158PurposeExpresses the Ct biosensor under a T7 promoterDepositorInsertT7-tRNA-Spinach2-Ct
ExpressionBacterialPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-Hook3 aa 1-552
Plasmid#74614PurposeExpresses a truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-552 (HOOK3 Human)
Tags6x His, Strep II, and superfolder GFPExpressionBacterialAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET22b_EcSSB_2
Plasmid#78204PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (W88C)
ExpressionBacterialMutationW88C (please see depositor comment below)PromoterT7Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-ffDronpa
Plasmid#78182PurposeExpresses ffDronpa E. coli strainsDepositorInsertffDronpa
Tags6xHis-tag; Xpress tagExpressionBacterialPromoterT7Available sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXa3
Plasmid#73614PurposeLight-induced nuclear export switch for microscopy in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLINXa3
TagsmCherryExpressionBacterial, Insect, and Mamm…PromoterChicken B-ActinAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAL_dstβL
Plasmid#73805PurposePlasmid for selection for insertion and homodimerizationDepositorInsertsCAT Marker
ToxR
beta lactamase
spectomycin resistance
CLS TM domain
TagsPart Of the dstbl chiemra, Part of the dsTbL chim…ExpressionBacterialPromoterNaN and ToxR promotorAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJK642
Plasmid#72391PurposeProduces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)DepositorInsertglutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
TagsHis6ExpressionBacterialMutationMutated tyrosine-397 to phenylalanine to block ad…PromoterT7Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJK615
Plasmid#71699PurposeProduces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)DepositorInsertthioredoxin
TagsHis6ExpressionBacterialPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-MSP1D1deltaH4/2
Plasmid#71712PurposeMembrane scaffold protein (MSP), half of helix 4 truncatedDepositorAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTKDP-cat
Plasmid#71321PurposeDonor plasmid - chloramphenicol resistant donor cassetteDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Gm-LacZ10
Plasmid#65026PurposeGmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 1. See Depositor Comments below for information regarding the lacZ sequence.DepositorPublicationTypeEmpty backboneTagslacZExpressionBacterialAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pET151-hUCH37 (ISF1)
Plasmid#61929PurposeExpression of hUCH37 (ISF1) in E.coliDepositorAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBW213ara-hrpS
Plasmid#61435PurposepBAD18-cm encoding rbs33-hrpS-terDepositorInsertrbs33-hrpS-ter
ExpressionBacterialPromoterpBADAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11a+CYP119
Plasmid#66131PurposeExpresses the wild type of CYP119DepositorAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by