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pRSETa_ mEos4b

Plasmid

#51073

Purpose

Photoconverible Fluorescent Protein_ Fixative Resistant (monomeric)

Depositor

Loren Looger

Article

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

mEos4b

Expression

Bacterial

Promoter

T7

Available Since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pET3a-Ub-K63R

Plasmid

#18898

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

Insert

Ubiquitin-K63R (UBC Human)

Expression

Bacterial

Mutation

Lysine 63 is replaced with a Argnine residue

Available Since

Sept. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-Cre-HiBiT

Plasmid

#198318

Purpose

Loads HiBiT-tagged Cre into PVCs using Pdp1 packaging domain; also contains Pnf

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-Cre-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-IQGAP1

Plasmid

#30107

Depositor

David Sacks

Article

Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

Insert

IQGAP1 (IQGAP1 Human)

Tags

GST

Expression

Bacterial

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huBsCas12a

Plasmid

#174671

Purpose

Expresses 6xHis-MBP tagged BsCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huBsCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET19-M(BRCT)TdTwt

Plasmid

#207459

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

M(BRCT)TdTwt (Dntt Mouse)

Tags

10xHis-MBP

Expression

Bacterial

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2+ EB3-mKate2

Plasmid

#105940

Purpose

in vitro transcription

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

EB3 (MAPRE3 Human)

Tags

mKate2

Expression

Bacterial

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-2/Rabaptin-5:R5BD

Plasmid

#168808

Purpose

Production of recombinant GST-R5BD (Rab5-binding domain) in bacteria for GST pulldown assays to identify binding partners for Rab5 subfamily members

Depositor

Guangpu Li

Article

Qi et al Methods Mol Biol. 2015;1298:259-70. doi:

Insert

Rab5-binding domain (aa739-862) of Rabaptin-5

Tags

GST

Expression

Bacterial

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLG024 (SIR2 + HerA)

Plasmid

#157902

Purpose

Expresses the SIR2 + HerA defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

SIR2 + HerA

Expression

Bacterial

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCool-mRbx1-His-hCul1-CTP

Plasmid

#29519

Depositor

Ning Zheng

Insert

Rbx1 CUL1 (CUL1, Rbx1 Mouse, Human)

Tags

GST, His (before thrombin), and Thrombin Cut Site…

Expression

Bacterial

Available Since

May 26, 2011

Availability

Academic Institutions and Nonprofits only
pCDM4

Plasmid

#49796

Purpose

ePathBrick vector for pathway engineering in E. coli.

Depositor

Mattheos Koffas

Article

Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

Type

Empty backbone

Tags

S-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pIB185

Plasmid

#90196

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

Depositor

Indranil Biswas

Article

Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

Type

Empty backbone

Tags

None

Expression

Bacterial

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDD56

Plasmid

#119883

Purpose

Arabinose inducible EYFP on pAL5000/pMB1 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pBAD_EYFP

Expression

Bacterial

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pWZ853

Plasmid

#208115

Purpose

mSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-BSD

Depositor

Jef Boeke

Article

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Insert

mNeonGreen-HPRT1-BSD

Tags

T2A

Expression

Bacterial and Mammalian

Promoter

EF1alpha

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP E17TAG

Plasmid

#68295

Purpose

E17TAG GFP reporter

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

E17TAG GFP

Tags

His

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pSW031

Plasmid

#231144

Purpose

Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.

Depositor

Philip Cole

Article

Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021

Insert

S. aureus Sortase A mutant

Tags

Nostoc punctiform split intein C-terminal fragmen…

Expression

Bacterial

Mutation

P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…

Promoter

T7

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA-RagC

Plasmid

#99641

Purpose

Bacterial expression of wildtype Rag GTPase dimer

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO2

Plasmid

#53142

Purpose

E.coli expression of untagged mature human SUMO2 (amino acids 1-92)

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

SUMO2 (SUMO3 Human)

Expression

Bacterial

Mutation

mature form (amino acids 1-92)

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pACM4

Plasmid

#49797

Purpose

ePathBrick vector for pathway engineering in E. coli.

Depositor

Mattheos Koffas

Article

Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

Type

Empty backbone

Tags

S-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 31, 2014

Availability

Academic Institutions and Nonprofits only

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His SUMO HNRNPA1 dHexa

Plasmid

#197013

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Molliex et al Cell. 2015 Sep 24;163(1):123-33. doi: 10

Insert

HNRNPA1

Tags

6His-SUMO

Expression

Bacterial

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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