30,307 results

1. pThermeleon

   Plasmid

   #86120

   Purpose

   Bandpass temperature-gated expression of mWasabi and mCherry reporters

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TlpA
   mWasabi
   TcI38
   mCherry
   cI
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T, M1V, L65S, K68R, F115L, D126G, D188G and D3…

   Promoter

   pR/pL, pR/pL, pLacI, and pTlpA

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRE-Tn7-142

   Plasmid

   #118558

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sGFP2
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTrcHisB-TRF2

   Plasmid

   #50488

   Purpose

   E. Coli N-term HexHis Tagged Protein Expression Vector

   Depositor

   Jack Griffith

   Article

   Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

   Insert

   TRF2 (TERF2 Human)
   

   Use

   Tags

   6x His, T7 tag, and TEV protease site

   Expression

   Bacterial

   Mutation

   Contains amino acids 3-500

   Promoter

   pTrc

   Available since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAWPSG-Ptet-nCas9-BE

   Plasmid

   #195739

   Purpose

   Express APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coli

   Depositor

   Seung-Goo Lee

   Article

   Jeong et al Chem Eng J, 2023

   Insert

   Tet repressor protein / APOBEC1-nCas9(D10A)-UGI
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet(Tetracycline inducible protein)

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6xHis-MBP-BrCas12b-FNLDTA

   Plasmid

   #195340

   Purpose

   Expresses Engineered BrCas12b in Bacteria

   Depositor

   Piyush Jain

   Article

   Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

   Insert

   BrCas12b
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis Tag and MBP, TEV cleavage site, 6x His Tag

   Expression

   Bacterial

   Mutation

   F208W, N524V, L795I, D868V, T874S, A1015E

   Promoter

   T7 Promoter

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE80L-IL2 C125S

   Plasmid

   #104348

   Purpose

   Expresses human IL-2 (C125S)

   Depositor

   Alexander McLellan

   Insert

   Human IL-2 (C125S) (IL2 Human)
   

   Use

   Tags

   MRGSHHHHHGS-tag

   Expression

   Bacterial

   Mutation

   C125S mutation (TGT -> TCC)

   Promoter

   T5

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBK092

   Plasmid

   #183146

   Purpose

   pET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expression

   Depositor

   Daan Swarts

   Article

   Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

   Insert

   CrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Otx1/pBS

   Plasmid

   #16332

   Depositor

   Peter Klein

   Insert

   Otx1 (otx1 Frog)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pET28b-miniE-DBCO

   Plasmid

   #181985

   Purpose

   Used to label proteins with the MiniR genetically encoded tag

   Depositor

   Kimberly Beatty

   Article

   Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

   Insert

   MiniE-DBCO
   

   Use

   Tags

   DBCO-Tag, His-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX6p-1-UFBP1

    Plasmid

    #204558

    Purpose

    Expresses GST-tagged UFBP1 in E.coli

    Depositor

    Masaaki Komatsu

    Article

    Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

    Insert

    UFBP1 (DDRGK1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. 6xHis-MBP-huErCas12a

    Plasmid

    #174685

    Purpose

    Expresses 6xHis-MBP tagged ErCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huErCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 6xHis

    Expression

    Bacterial

    Mutation

    Q926R mutation is observed as indicated from the …

    Promoter

    T7

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJBM3

    Plasmid

    #225290

    Purpose

    E. coli-Campylobacter shuttle vector; KanR

    Depositor

    Robert Moore

    Article

    McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

    Insert

    Replication elements from pCC388 for pJBM3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbS5c-Maqu_3494

    Plasmid

    #86205

    Purpose

    Expresses efflux pump in E. coli to provide resistance to biofuel (low copy)

    Depositor

    Mary Dunlop

    Article

    Turner et al ACS Synth Biol. 2015 Oct 16;4(10):1056-6

    Insert

    Efflux pump
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. His-tagged FL Rec8

    Plasmid

    #45243

    Purpose

    raise antibody against Rec8

    Depositor

    Michael Lampson

    Article

    Chiang et al Curr Biol. 2010 Sep 14;20(17):1522-8. do

    Insert

    Rec8 (Rec8 Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. SurA

    Plasmid

    #213138

    Purpose

    Expresses chaperone SurA

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    SurA (21-428) (surA E.coli)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMMB67EH-Spy

    Plasmid

    #90102

    Purpose

    IPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.

    Depositor

    Samuel Miller

    Article

    Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

    Insert

    YcgR (ycgR )
    

    Use

    Broad-host range

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pA5c-tesA-vhb50-8fadR

    Plasmid

    #86601

    Purpose

    expression of tesA and Vhb50 under the control of a LacUV5 promoter and fadR under the control of a pBAD promoter

    Depositor

    Yinjie Tang

    Article

    Liu et al Biotechnol Bioeng. 2016 Aug 6. doi: 10.1

    Inserts

    tesA
    Vhb50
    fadR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacUV5 and pBAD

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2

    Plasmid

    #183741

    Purpose

    Expresses Ki67 (8 repeats+LR)-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    MKI67 LR domain + 8 Ki-67 repeats

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28C-mCherry-DDX4LCD

    Plasmid

    #204408

    Purpose

    E. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    DDX4(1-236) (DDX4 Human)
    

    Use

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pReporter_12

    Plasmid

    #60572

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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