30,803 results
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Plasmid#134931PurposeGEF-H1 biosensor that has GEF activity.DepositorInsertGEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)
TagsNone.ExpressionBacterial and MammalianPromoterCMV and T7Available SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-TorA(sp)-mNeonGreen
Plasmid#205020PurposeBroad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasmDepositorInsertTorA-mNeonGreen
ExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable SinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-PAmCherry1
Plasmid#31931DepositorInsertPAmCherry1
TagsHisExpressionBacterialMutationE26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…PromoteraraBAvailable SinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI
Plasmid#115214PurposeExpresses PauCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPauCas13
TagsSUMOExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-H12TEV
Plasmid#208635Purposefor E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.DepositorTypeEmpty backboneTags6xhisExpressionBacterialPromoterT7Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-BB0323-ss
Plasmid#137067PurposeE. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66700.1
TagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature BB0323: lacks the BB0323 signal peptidePromoterT7lacAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-3-SARS-CoV-2-3CL
Plasmid#168457PurposeBacterial codon optimized expression vector for SARS-CoV-2 3CL protease.DepositorAvailable SinceMarch 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKSJ340
Plasmid#27125DepositorInsertColicin Ia + Ia immunity protein
ExpressionBacterialAvailable SinceJan. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pet16b-Avi-ubiquitin_rbs_BirA
Plasmid#134897PurposeExpresses HIS-Avi-tagged ubiquitin for recombinant protein productionDepositorInsertsTags10HIS-AviTag-PreScission(3C)proteaseExpressionBacterialPromoterRBS and T7Available SinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pkk223_Null
Plasmid#213111Purposepkk223 backbone plasmidDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
Beta-Lactamase
Plasmid#62729PurposeExpresses Beta-Lactamase protein in bacterial cellsDepositorInsertBeta-Lactamase
TagsHis tagExpressionBacterialMutationDeletion of periplasmic signal domainPromoterT7Available SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sfGFP-DQNAT
Plasmid#198210PurposepJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tagDepositorInsertsfGFP_DQNAT
Tags6x His Tag and DQNAT glycosylation tagExpressionBacterialPromoterT7Available SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYTK050
Plasmid#65157PurposeEncodes sgRNA Dropout as a Type 234 part to be used in the Dueber YTK systemDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZP06
Plasmid#232320PurposepZP05 was constructed by cloning the oriFn-Pfdx-E-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, creating a conditional plasmid with replication controlled by Pfdx-E.DepositorInsertPfdx-E
ExpressionBacterialAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG GST Empty
Plasmid#236735PurposeEmpty bacterial expression plasmid for GST fusion proteinsDepositorTypeEmpty backboneTagsGSTExpressionBacterialAvailable SinceApril 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAG GST EGFP
Plasmid#236736PurposeBacterial expression plasmid containing GST-tagged EGFPDepositorInsertEGFP
TagsGSTExpressionBacterialPromoterTacAvailable SinceApril 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKEK3395
Plasmid#234079PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertYukonOFP
ExpressionBacterialPromoterpJ23100-BsaIAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+) pCopA RBS30 mscarlet
Plasmid#226370PurposePlasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30DepositorInsertmScarlet
Tags6x HistagExpressionBacterialPromotercopA promoter (pCopA)Available SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10
Plasmid#198450PurposeProduces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody; also contains truncated spike tip protein (Pvc10).DepositorInsertPVCpnf_pvc13-antiMouseMHCIINb_Δpvc10
ExpressionBacterialAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 uvrA
Plasmid#112892PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertuvrA-like protein
Tags6xHisExpressionBacterialPromoterPblaAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only