32,069 results
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Plasmid#218952PurposeExpresses proinsulin fused to EYFP in bacteriaDepositorInsertproinsulin-EYFP
TagsproinsulinExpressionBacterialPromoterT5Available SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBx-Spas-sgRNA-Gm
Plasmid#105232PurposePlasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosaDepositorInsertS. pasteurianus sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGR596
Plasmid#213787PurposeIntegrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassetteDepositorInsertBadBoy2 TP-DNAP1
UseSynthetic BiologyExpressionBacterial and YeastMutationSee manuscriptAvailable SinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>
Plasmid#72240PurposeHistidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)DepositorInsertGLT1 GAL1p 24xPP7 V3
ExpressionBacterialAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
AURKB
Plasmid#39119PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLG031 (upx)
Plasmid#157909PurposeExpresses the upx defense systemDepositorInsertupx (putative PD-(D/E)xK family phosphodiesterase)
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTL032
Plasmid#80928Purpose14x MS2 sense + 14x PP7 antisense with loxP-kanMX-loxPDepositorInsert14xMS2 sense + 14xPP7 antisense, loxP-kanMX-loxP
ExpressionBacterialAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLG027 (qatABCD)
Plasmid#157905PurposeExpresses the qatABCD defense systemDepositorInsertqatABCD (ATPase + unknown protein + QueC homolog + TatD DNase)
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET41b+_Ufd1-His
Plasmid#117107PurposeProduction of recombinant Ufd1-His in E.coliDepositorAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTn7xTS_SpyCatcher003:mNG
Plasmid#225190PurposeUsed for inserting SpyCatcher003 fused to mNeonGreen at the bacterial Tn7 siteDepositorInsertSpyCatcher003:mNeonGreen
TagsSpyCatcherExpressionBacterialAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAX1_HS_gpN:SpyTag003
Plasmid#225194PurposeUsed for adding C-terminal SpyTag003 to the E. coli HS P2 phage major capsid proteinDepositorInsertgpN homology arms and SpyTag003
ExpressionBacterialAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_NSD2
Plasmid#221376PurposeExpresses NSD2 in bacterial cellsDepositorAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDHP_YEAST
Plasmid#204233PurposeBacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHP_YEAST (MDH3 Budding Yeast)
Tags6X HisExpressionBacterialMutationThe last 3 C-terminal residues (SKL) are the pero…PromoterLacAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
28A_APEX2_MSP1D1
Plasmid#170923PurposeExpress APEX2_MSP1D1 in Bl21DepositorInsertAPEX2-MSP
ExpressionBacterialAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETa_ mEos4b
Plasmid#51073PurposePhotoconverible Fluorescent Protein_ Fixative Resistant (monomeric)DepositorInsertmEos4b
ExpressionBacterialPromoterT7Available SinceFeb. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSB233
Plasmid#164128PurposePP7-CFP and PhlF-RFP expression plasmidDepositorInsertPP7-CFP
ExpressionBacterialPromoterPtetAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_wt_Venus_wt
Plasmid#39813DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
PET-eSpCas9-NLS-6xHis
Plasmid#207379PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInserteSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationK848A, K1003A, R1060APromoterT7Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK603
Plasmid#8831DepositorInsertTEV protease, S219D mutant
ExpressionBacterialMutationS219D mutantAvailable SinceJune 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pWUR703
Plasmid#53082Purposeinducible TtAgoDM (D478A,D546A) expressionDepositorInsertTtAgo
TagsStrep-II tagExpressionBacterialMutationD478A D546APromoterT7Available SinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only