31,794 results

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pSIBR004

Plasmid

#177667

Purpose

SIBR Intron 4 (Int4) interrupts the CDS of FnCas12a

Depositor

John van der Oost

Article

Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

lacUV5

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pQE9-His-p97(wt)

Plasmid

#14666

Depositor

Graham Warren

Article

Meyer et al EMBO J. 2000 May 15. 19(10):2181-92.

Insert

p97 (Vcp Mouse)

Tags

RGS6xHis

Expression

Bacterial

Mutation

See depositor comments below.

Available Since

April 13, 2007

Availability

Academic Institutions and Nonprofits only

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PGEX-NEDD4

Plasmid

#45043

Depositor

Shiaw-Yih Lin

Article

Yim et al Cancer Cell. 2009 Apr 7;15(4):304-14. do

Insert

NEDD4-1 (NEDD4 Human)

Tags

GST

Expression

Bacterial

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
pH6HTC_IGF2BP2a-Halo

Plasmid

#175309

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

IGF2BP2 (IGF2BP2 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTD103aiiA(Cm)

Plasmid

#48886

Purpose

Negative feedback plasmid from oscillator

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

aiiA and LuxR

Use

Synthetic Biology

Tags

ssrA tag (AANDENYALAA)

Expression

Bacterial

Promoter

pLux

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pQE30-HyPerFLEX

Plasmid

#217569

Purpose

Bacterial expression of HyPerFLEX

Depositor

Vsevolod Belousov

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX

Tags

6xHis

Expression

Bacterial

Promoter

T5

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pTW335p3

Plasmid

#237329

Purpose

expresses Z. mays RbcL RbcS-6xHis

Depositor

Tina Wang

Article

Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

Inserts

Z. mays RbcL
Z. mays RbcS-6xHis

Tags

6xHis and None

Expression

Bacterial

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET24a_SMOX

Plasmid

#186345

Purpose

C-terminal His-Tag and a TEV cleaveage site, to transform into E. coli BL21 (DE3) cells

Depositor

Sandra Gabelli

Article

Dunston et al Acta Naturae. 2020 Jul-Sep;12(3):140-144

Insert

Spermine oxidase (SMOX Human)

Tags

His tag

Expression

Bacterial

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJUMP29-1A(lacZ)

Plasmid

#126978

Purpose

Level 1 vector. With lacZ as alternative cloning reporter. OriV 9 (pBBR322/ROP; medium copy number).

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pPEP-TEV cNup98 (1-498)

Plasmid

#38037

Depositor

Roderick Lim

Article

Kowalczyk et al Nat Nanotechnol. 2011 Jun 19;6(7):433-8.

Insert

Nucleoporin 98 (NUP98 Human)

Tags

His-tag, 3Cysteine

Expression

Bacterial

Mutation

amino acids 1-498

Promoter

T7

Available Since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pLG002 (BREX type I)

Plasmid

#157880

Purpose

Expresses the BREX type I defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

BREX type I

Expression

Bacterial

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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AAK1

Plasmid

#73244

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

AAK1 (AAK1 Human)

Tags

TEV-His6-Flag

Expression

Bacterial

Mutation

pfam00069:Pkinase 47-307

Promoter

T7

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
pET28-2H12.D11

Plasmid

#221145

Purpose

His6-tagged cdGreen2 expressed from T7 promoter (Internal Lab ID: UJ11357)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen2

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMRS-BG42-GFP

Plasmid

#220202

Purpose

mini-Tn7 GFP expression vector - pBG42 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG42

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcI-ts,ind+-(mtSSB delta20)

Plasmid

#186280

Purpose

Expresses Human Mitochondrial SSB in E. coli

Depositor

Kenneth Johnson

Article

Qian et al J Biol Chem. 2017 Aug 4;292(31):13068-13

Insert

Mitochondrial Single Stranded DNA Binding Protein (SSBP1 Human)

Expression

Bacterial

Mutation

First 20 amino acids corresponding to probable mi…

Promoter

lambda

Available Since

July 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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p3990 pET ubcH8

Plasmid

#8650

Depositor

Peter Howley

Article

Kumar et al J Biol Chem 1997 May 23;272(21):13548-54

Insert

UbcH8 (UBE2L6 Human)

Tags

His

Expression

Bacterial

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
pRBBm34

Plasmid

#48114

Purpose

Expression of the gfp gene under control of the native xylose inducible promoter of Bacillus megaterium

Depositor

Dieter Jahn

Article

Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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HSA2_His

Plasmid

#202764

Purpose

human serum albumin design HSA2, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

HSA2

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLQ-Pxyl/tet-cas9-Pj23119-sgRNA

Plasmid

#92121

Purpose

CRISPR-Cas9 based efficient genome editing in S. aureus

Depositor

Sheng Yang

Article

Liu et al Acta Biochim Biophys Sin (Shanghai). 201

Insert

Cas9-Pxyltet-sgRNA-pj23119

Use

CRISPR

Expression

Bacterial

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-HA

Plasmid

#198296

Purpose

Produces PVCs retargeted with HA tag

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-HA

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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