29,776 results

1. pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP

   Plasmid

   #200277

   Purpose

   Characterisation of the sialic acid-responsive circuit with fluorescent reporter

   Depositor

   Matthew Chang

   Article

   Koh et al Nat Commun. 2022 Jul 4;13(1):3834. doi:

   Inserts

   CadC
   NanR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. nGCaMP2-NpuDnaEn

   Plasmid

   #34850

   Depositor

   Kevin Truong

   Article

   Wong et al Cell Calcium. 2011 Nov 29.

   Insert

   nGCaMP2-NpuDnaEn
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV, T7 lac

   Available since

   March 27, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pSUMO His6 SUMO MYC3

   Plasmid

   #177848

   Purpose

   Bacterial Expression of MYC3

   Depositor

   Karsten Melcher

   Article

   Zhang et al Nature. 2015 Sep 10;525(7568):269-73. do

   Insert

   MYC3 (MYC3 Mustard Weed)
   

   Use

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pProEx M-Ras(G22C) CL

   Plasmid

   #224268

   Purpose

   Protein purification of M-Ras (G22C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   M-Ras (MRAS Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G22C

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pProEx Rit1(G47C) CL

   Plasmid

   #224267

   Purpose

   Protein purification of Rit1 (G47C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   Rit1 (RIT1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G47C

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pProEx Ypt1(S17C) CL

   Plasmid

   #224265

   Purpose

   Protein purification of Ypt1 (S17C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   Ypt1 (YPT1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S17C

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pProEx Rap1A(G12C, L96F) CL

   Plasmid

   #224264

   Purpose

   Protein purification of Rap1A (G12C, L96F)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   Rap1A (RAP1A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G12C, L96F

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pProEx RalA(G23C) CL

   Plasmid

   #224263

   Purpose

   Protein purification of RalA (G23C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   RalA (RALA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G23C

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Mucin MUC1-5TR-pET15b

   Plasmid

   #174694

   Purpose

   Plasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)

   Depositor

   Jean Gariepy

   Article

   Brokx et al Biochemistry. 2003 Dec 2;42(47):13817-25

   Insert

   MUC1-TR
   

   Use

   Tags

   hexahistidine (HIS) tag

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7 Promoter

   Available since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNIC-CT-sfGFP-HN-GG

    Plasmid

    #215803

    Purpose

    Cloning of target gene with C terminal TEV cleavable sfGFP HisAsn6 tag

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pRSET.GltI253-cpGFP.L1LV/L2NP

    Plasmid

    #41733

    Purpose

    a single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expression

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

    Insert

    GltI based Glutamate Biosensor
    

    Use

    Glutamate biosensor

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Bacterial

    Mutation

    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

    Promoter

    T7

    Available since

    Jan. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCri-1b

    Plasmid

    #61327

    Purpose

    Protein expression.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    His-tag and MBP-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pLEVI(408)-ColE-iLight-msfGFP

    Plasmid

    #170268

    Purpose

    Expresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    iLight
    

    Use

    Tags

    msfGFP

    Expression

    Bacterial

    Mutation

    To design iLight for expression in bacteria, a fu…

    Promoter

    pJ23116

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a_Pro-IL-37

    Plasmid

    #214346

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL37 (IL37 Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHEA

    Plasmid

    #168297

    Purpose

    Bacterial surface display of polypeptides (nanobodies) in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Marin et al J Bacteriol. 2010 Nov;192(21):5588-602.

    Insert

    Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
    

    Use

    Tags

    6xHis, E-tag, and PelB signal peptide

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMT-ADARcd-E488Q-V5

    Plasmid

    #139686

    Purpose

    Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5

    Depositor

    Michael Rosbash

    Article

    Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

    Insert

    Adenosine deaminase acting on RNA (Adar Fly)
    

    Use

    Tags

    V5

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC8 human IFN-gamma

    Plasmid

    #17600

    Depositor

    Howard Young

    Article

    Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

    Insert

    interferon-gamma cDNA (IFNG Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2012

    Availability

    Academic Institutions and Nonprofits only

18. NdeI – SacII – SpeI(NheI) - ANXA2 (23-339)

    Plasmid

    #136543

    Purpose

    Expresses ANXA2 chimeras in bacterial cells

    Depositor

    Laszlo Nyitray

    Article

    Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

    Type

    Empty backbone

    Use

    Tags

    ANXA2 (23-339) and His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21-pelB-LaG-30

    Plasmid

    #172754

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-30
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. BB1_12_pGAP

    Plasmid

    #98498

    Purpose

    BB1 with insert: promoter PGAP

    Depositor

    Michael Sauer, Brigitte Gasser, Diethard Mattanovich

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Insert

    GAP promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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