30,803 results

1. pGDP1 stat

   Plasmid

   #112886

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   stat
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   Pbla

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. IVT-MCP-Phi29

   Plasmid

   #207456

   Purpose

   in vitro transcription of mRNA

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

   Insert

   MCP-Phi29
   

   Expression

   Bacterial

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas-Cas12m

   Plasmid

   #192274

   Purpose

   Encodes MmCas12m under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmCas12
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PJ23108

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p11d-tRPA (MSW#111)

   Plasmid

   #208066

   Purpose

   expression of human Replication Protein A in E. coli

   Depositor

   Marc Wold

   Article

   Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

   Insert

   RPA1, RPA2, RPA3 (RPA1, RPA3, RPA2 Human)
   

   Expression

   Bacterial

   Mutation

   RPA32 has a point mutation Thr88(ACC) -> Ile(A…

   Available Since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJL1 ATF1

   Plasmid

   #106286

   Purpose

   Expresses ATF1 in Ecoli off a T7 promoter

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   ATF1
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCL1333

   Plasmid

   #217966

   Purpose

   Bacterial expression of RAN with an N-terminal 6His tag and a TEV cleavage site.

   Depositor

   Andrew Bowman

   Article

   Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

   Insert

   RAN (RAN Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRF-AmtR

   Plasmid

   #49355

   Depositor

   Christopher Voigt

   Article

   Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

   Insert

   AmtR repressor
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. hGAPDH-rLuc

   Plasmid

   #82479

   Purpose

   reporter of GAPDH expression

   Depositor

   Albee Messing

   Article

   Cho et al J Neurochem. 2009 Jul;110(1):343-51. Epu

   Insert

   GAPDH promoter (GAPDH Human)
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   hGAPDH

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

   Plasmid

   #216283

   Purpose

   Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SpyCatcher003-TEVs-SpyTag003DA
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAKlux3

    Plasmid

    #14082

    Depositor

    Attila Karsi

    Article

    Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

    Insert

    lacIq repressor gene
    

    Expression

    Bacterial

    Available Since

    March 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPaGE Pyl TAG FliC T248TAG

    Plasmid

    #160089

    Purpose

    Encodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.

    Depositor

    Lital Alfonta

    Article

    Ozer et al Sci Adv. 2021 Jun 11;7(24). pii: 7/24/ea

    Inserts

    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Flagellin
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Changed Threonine 248 to TAG stop-codon.

    Promoter

    Pseudomonas aeruginosa Leu-tRNA native promoter a…

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ub-GyrA

    Plasmid

    #128737

    Purpose

    Expresses Ub fused to GyrA intein at the C-terminal

    Depositor

    Sharon Rozovsky

    Article

    Liu et al Chembiochem. 2019 Jul 2. doi: 10.1002/cb

    Insert

    UBC
    

    Tags

    Mxe GyrA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 18, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. C1-82 pComb3xss anti-cFos nanobody

    Plasmid

    #145829

    Purpose

    Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-cFos (Human) recombinant llama nanobody C1-82 (FOS L. glama (llama))
    

    Tags

    6xHis-HA

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX4T3-GST-NQO1

    Plasmid

    #177140

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of NQO1 with a TEV protease site located between the GST tag and NQO1

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    NQO1 (NQO1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pST-KiT

    Plasmid

    #44562

    Purpose

    for integrative inducible expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28b-GBP-MTn

    Plasmid

    #154363

    Purpose

    Bacterial expression of MTn

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    MTn
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28-MKH8SUMO

    Plasmid

    #79526

    Purpose

    Bacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    8xHis-Thrombin-SUMO-TEV and TEV cleavage site

    Expression

    Bacterial

    Available Since

    June 16, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

18. Twitch-2B pRSETB

    Plasmid

    #48203

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-2B
    

    Tags

    6xHIS, cpVenusCD, and mCerulean3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pS23·Pm-GFP

    Plasmid

    #122593

    Purpose

    Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    Pm promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Pm

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. APneg-VAMP1a

    Plasmid

    #168007

    Purpose

    negative selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    VAMP1(T29-K89)-T7PAP
    

    Expression

    Bacterial

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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