30,306 results

1. R717-X45-566: His6-MBP-tev-Hs.RAF1(136-188)

   Plasmid

   #159698

   Purpose

   E. coli protein expression of His6-MBP-tev-Hs.RAF1(136-188)

   Depositor

   Dominic Esposito

   Article

   Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

   Insert

   His6-MBP-tev-Hs.RAF1(136-188) (RAF1 Human)
   

   Use

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pST44-AP-1 core

   Plasmid

   #184809

   Purpose

   Bacterial expression of clathrin adaptor AP-1 core complex

   Depositor

   James Hurley

   Article

   Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

   Insert

   Hs AP1S3; Hs AP1B1 (1-584); Mm AP1G1 (1-595); Mm AP1M1
   

   Use

   Tags

   GST tag and His6

   Expression

   Bacterial

   Mutation

   AP1S3: S148C; AP1B1: K359R, E476K

   Promoter

   T7

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. DCX-RFP

   Plasmid

   #32851

   Depositor

   Joseph Gleeson

   Article

   Tanaka et al J Cell Biol. 2004 Jun 7. 165(5):709-21.

   Insert

   DCX (DCX Human)
   

   Use

   Tags

   DsRed2

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pMyBADNT-kan

   Plasmid

   #84691

   Purpose

   Protein expression in mycobacteria

   Depositor

   Matthias Wilmanns

   Article

   Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   AraC

   Available since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-Sac-Abeta(MC1-42)

   Plasmid

   #127151

   Purpose

   Expresses N-terminal cysteine Abeta(1-42)

   Depositor

   James Nowick

   Article

   Zhang et al Biochemistry. 2021 Apr 20;60(15):1191-12

   Insert

   Abeta(MC1-42) (APP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFN18A-HaloTag-SpyCatcher

   Plasmid

   #206041

   Purpose

   HaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTag

   Depositor

   Sergi Garcia-Manyes

   Article

   Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

   Type

   Empty backbone

   Use

   Tags

   HaloTag and SpyCatcher-8His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-SUMO-mGSDMD

   Plasmid

   #111560

   Purpose

   Express mouse GSDMD in E.coli

   Depositor

   Hongbo Luo

   Article

   Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

   Insert

   Gasdermin D (Gsdmd Mouse)
   

   Use

   Tags

   His, SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pExchange_tdk_BT1311p-tetR-1

   Plasmid

   #90325

   Purpose

   Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 4861701 and 4861702

   Depositor

   Andrew Goodman

   Article

   Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

   Insert

   TetR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFLxR3

   Plasmid

   #167518

   Purpose

   Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Tetracycline Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   LuxR-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLuxB

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-APOBEC1-YTH

    Plasmid

    #200158

    Purpose

    For bacterial expression of the APOBEC1-YTH fusion protein for protein purification

    Depositor

    Kate Meyer

    Article

    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

    Insert

    APOBEC1-YTH
    

    Use

    Tags

    6xHis, HA, and Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    Codon optimized for E coli expression

    Promoter

    LacZ

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJOE_pvdJ

    Plasmid

    #110142

    Purpose

    Suicide plasmid with repair template for knockout in P. putida

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBAD/HisB-iRFP670

    Plasmid

    #45453

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP670
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    araBAD

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPP381

    Plasmid

    #158798

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    dCasPhi-2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    RuvC-inactivating mutation D394A

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28_human-Caspase-4_enzymatic-domain

    Plasmid

    #183381

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-4 (AA94-377) (CASP4 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pREDKI

    Plasmid

    #51626

    Purpose

    E coli Genetic Modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRha

    Available since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p3523 pGEM HPV-11 E6

    Plasmid

    #10854

    Depositor

    Peter Howley

    Article

    Werness et al Science. 1990 Apr 6. 248(4951):76-9.

    Insert

    HPV11 E6
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pTD103-ARG1

    Plasmid

    #106475

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG1) in Salmonella typhimurium

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLux

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEM8317

    Plasmid

    #213989

    Purpose

    PrpsM driven expression of sfGFP

    Depositor

    Marc Erhardt

    Article

    Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrpsM

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNIC-CTHF

    Plasmid

    #26105

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

    Type

    Empty backbone

    Use

    Tags

    TEV - His6 - Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-4T-1 3xFlag-CREB

    Plasmid

    #82721

    Purpose

    Expresses human CREB

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    CREB (CREB1 Human)
    

    Use

    Tags

    3XFLAG and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge