30,535 results

1. pGEx-4T-1-YTHDF1

   Plasmid

   #70087

   Purpose

   Encode YTHDF1 with N-terminal GST tag for E. coli expression

   Depositor

   Chuan He

   Article

   Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

   Insert

   YTH N(6)-methyladenosine RNA binding protein 1 (YTHDF1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSC168

   Plasmid

   #216350

   Purpose

   xhNup35-Nb1t (+2Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xhNup35-Nb1t (+2Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRE132

   Plasmid

   #118486

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sGFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRAM18dRGA[MCS]

   Plasmid

   #84683

   Purpose

   Can be used for the transformation of rickettsia

   Depositor

   Ulrike Munderloh

   Article

   Burkhardt et al PLoS One. 2011;6(12):e29511. doi: 10.137

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET16b-Trx

   Plasmid

   #36944

   Depositor

   Anna Blom

   Article

   King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

   Insert

   Thioredoxin 1 (TXN Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIRCgp41-1

   Plasmid

   #74227

   Purpose

   Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   gp41-1 C-intein and gp41-1 N-intein

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lac

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. NNMT

   Plasmid

   #40734

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   NNMT (NNMT Human)
   

   Use

   Tags

   His Thrombin

   Expression

   Bacterial

   Mutation

   K100A:E101A:E103A

   Promoter

   Available Since

   Oct. 18, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pOlyA(E69A)

   Plasmid

   #132392

   Purpose

   Mutant version of OlyA that senses sphingomyelin without or with cholesterol

   Depositor

   Arun Radhakrishnan

   Article

   Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

   Insert

   Ostreolysin A point mutant (E69A)
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Mutations: C62S, E69A, C94S, S151C

   Promoter

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. IL13 promoter luciferase

   Plasmid

   #11784

   Depositor

   Anjana Rao

   Article

   Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

   Insert

   IL-13 promoter (IL13 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 4, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pET-45b-mCherry-NPM1

    Plasmid

    #194546

    Purpose

    Bacterial Expression of 6xHis mCherry-NPM1 Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    NPM1 (Npm1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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