29,900 results

1. pNBU2_erm_P1T_DP-GH023

   Plasmid

   #90317

   Purpose

   Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023

   Depositor

   Andrew Goodman

   Article

   Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pProEx M-Ras(G22C) CL

   Plasmid

   #224268

   Purpose

   Protein purification of M-Ras (G22C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   M-Ras (MRAS Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G22C

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pProEx Rit1(G47C) CL

   Plasmid

   #224267

   Purpose

   Protein purification of Rit1 (G47C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   Rit1 (RIT1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G47C

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pProEx Ypt1(S17C) CL

   Plasmid

   #224265

   Purpose

   Protein purification of Ypt1 (S17C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   Ypt1 (YPT1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S17C

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pProEx Rap1A(G12C, L96F) CL

   Plasmid

   #224264

   Purpose

   Protein purification of Rap1A (G12C, L96F)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   Rap1A (RAP1A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G12C, L96F

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pProEx RalA(G23C) CL

   Plasmid

   #224263

   Purpose

   Protein purification of RalA (G23C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   RalA (RALA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G23C

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNIC-CT-sfGFP-HN-GG

   Plasmid

   #215803

   Purpose

   Cloning of target gene with C terminal TEV cleavable sfGFP HisAsn6 tag

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. NpuDnaEc-cGCaMP2

   Plasmid

   #34851

   Depositor

   Kevin Truong

   Article

   Wong et al Cell Calcium. 2011 Nov 29.

   Insert

   NpuDnaEc-cGCaMP2
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV, T7 lac

   Available since

   June 21, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pSK+ chickBMPR-1B DN

   Plasmid

   #49529

   Purpose

   cloning vector for dominant negative chick BMP receptor 1B

   Depositor

   Lee Niswander, Peter ten Dijke

   Article

   Zou et al Genes Dev. 1997 Sep 1;11(17):2191-203.

   Insert

   BMPR-1B DN (BMPR1B Chicken)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   dominant negative mutation (K231R); also P2A, M11…

   Promoter

   T3

   Available since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pBAD-Citron1

    Plasmid

    #134300

    Purpose

    Bacterial expression of direct-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citron1
    

    Use

    Tags

    6-His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETM6-HpaBC

    Plasmid

    #71260

    Purpose

    Expresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6

    Depositor

    Mattheos Koffas

    Article

    Jones et al Biotechnol Prog. 2015 Oct 21. doi: 10.10

    Inserts

    HpaB
    HpaC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRT498-OtUBD(V203D)

    Plasmid

    #190090

    Purpose

    Expresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

    Depositor

    Mark Hochstrasser

    Article

    Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

    Insert

    OtUBD(V203D)
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDT386

    Plasmid

    #174733

    Purpose

    Expresses His-tagged DT386, a truncated diphtheria toxin without a receptor-binding domain.

    Depositor

    Jukka Finne

    Article

    Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-

    Insert

    DT386
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Deleted amino acids 412-560 (the receptor binding…

    Promoter

    T5

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Zika NS5 RdRp

    Plasmid

    #204788

    Purpose

    Bacterial expression of Zika NS5 RdRp

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Zika NS5 RdRp domain
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pQE-60NA-proinsulin-mEGFP

    Plasmid

    #160464

    Purpose

    Encodes a fusion of human proinsulin and the monomeric enhanced EGFP. This construct can be used as a negative control for superfolder activity in bacteria

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    proinsulin-mEGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. RNF146-c005

    Plasmid

    #173091

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    RNF146 (RNF146 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    R99-L183, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a(+)::MBP-NL3F10H

    Plasmid

    #141291

    Purpose

    MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    MBP-NLUC3F10H
    

    Use

    Luciferase

    Tags

    Flag (x3), His (x10), and MBP

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. EV-D68 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

    Plasmid

    #204817

    Purpose

    Bacterial expresison of Enterovirus D68 3C protease

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    EV D68 3C protease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. BPK2101

    Plasmid

    #65770

    Purpose

    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS-3xFlag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDD108

    Plasmid

    #119884

    Purpose

    Integration vector into the ROCI-2 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance marker

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    BbaJ23104_EYFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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