30,260 results
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Plasmid#182716PurposeConstituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloningDepositorInsertCasRx crRNA cloning backbone
UseCRISPRTagsGFP in cloning site of crRNA for easier screening…ExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)Available sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
human EB1 in PET28a for protein expression
Plasmid#39297DepositorInsertEB1 (MAPRE1 Human)
UseTags6HISExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNIC-AncCAR_A
Plasmid#168286PurposeExpresses AncCAR_A in E. coli from a T7 promoterDepositorInsertAncCAR_A
UseSynthetic BiologyTags6*HisExpressionBacterialMutationPromoterT7Available sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
p4305
Plasmid#198150Purposebacterial expression of sifA::HaloTag fusionDepositorInsertPsifA sifA::L16::HaloTag::HA (sifA Salmonella enterica serovar Typhimurium)
UseTagsHaloTag-HAExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSR1038
Plasmid#208737PurposeCharacterization plasmid for PCap (sfgfp expressed from PCap on the S. aureus pC194 replicon)DepositorInsertPCap
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCapAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD/His-miRFP670nano
Plasmid#127427PurposeMonomeric near-infrared fluorescent protein miRFP670nano in bacterial plasmidDepositorInsertmiRFP670nano
UseTagsHisExpressionBacterialMutationPromoteraraCAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3187 HPV-16 E2
Plasmid#10846DepositorInsertHPV-16 E2 (E2 Human papillomavirus)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pEN017-SpCas9-hiNLS-t-M4
Plasmid#234455PurposeExpresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGGDepositorInsertSpCas9 hiNLS t-M4
UseCRISPRTagsHis6, Nucleoplasmin, SV40, and c-MycExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …PromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEN019-SpCas9-hiNLS-t-M1M4
Plasmid#234456PurposeExpresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGGDepositorInsertSpCas9 hiNLS t-M1M4
UseCRISPRTagsHis6, Nucleoplasmin, SV40, and c-MycExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …PromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGGG-Sr43
Plasmid#186974PurposeSr43 is a wheat stem rust resistance gene transferred from tall wheat grass (Thinopyrum ponticum) into bread wheat (Triticum aestivum).DepositorInsertSr43
UseTagsNoneExpressionBacterial and PlantMutationNonePromoterNaticeAvailable sinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
PJQ49
Plasmid#134657PurposeContains Promoter 3 with bsa1 cut sites for inserting sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAnderson BBa_J23115Available sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_47
Plasmid#109245PurposepSC101 var5 BbsI cloning vectorDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPJ23105Available sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
CD Ugi4
Plasmid#177196PurposeGreengate entry vector pGGC000 modified to contain bladderwort insulator 4DepositorInsertUtricularia gibba insulator 4
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceJune 28, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
RARS_pNIC-Bio3
Plasmid#153055PurposeExpression construct for producing Avi tagged Arginyl-tRNA synthetaseDepositorInsertRARS (RARS1 Human)
UseTagsAvi and His6-TEVExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBRα-σ70 D581G
Plasmid#53732PurposePlpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.DepositorInsertRNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
UseTagsExpressionBacterialMutationThe sigma 70 moiety carries the D581G substitutionPromoterlpp/lacUV5 (tandem promoter)Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMYCO1_SpdCas9_pmcDA1
Plasmid#204615PurposeCRISPR cytosine deaminase replicative plasmid for Mycoplasma mycoides subsp. mycoidesDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaE (pESC124)
Plasmid#50390PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.DepositorInsertDPCK
UseTags6XHIS tagExpressionBacterialMutationPromoterT7Available sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETM33_EIF4E_eiF4E
Plasmid#178474PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertEIF4E_eiF4E (EIF4E Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRBAD-Z-CGFP
Plasmid#52735PurposeL-(+)-arabinose-inducible expression of Z-CGFP positive control for in vivo split GFP assembly assayDepositorInsertZ+CGFP
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only