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pMV306hsp+Lux

Plasmid

#26159

Depositor

Brian Robertson, Siouxsie Wiles

Article

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

Bacterial luciferase operon

Expression

Bacterial

Mutation

It contains a gram-positive enhanced translation …

Available Since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pET His6 NusA TEV LIC cloning vector (1N)

Plasmid

#29657

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, NusA, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_45bpLinker

Plasmid

#157791

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_45bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2(+)-zGrad

Plasmid

#119716

Purpose

Plasmid to generate zGrad mRNA to inactivate proteins in zebrafish

Depositor

Holger Knaut

Article

Yamaguchi et al Elife. 2019 Feb 8;8. pii: 43125. doi: 10

Insert

zGrad

Expression

Bacterial

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pTGV-Express-CAT SMR5916

Plasmid

#28039

Depositor

Susan Rosenberg

Article

Gumbiner-Russo et al Gene. 2001 Jul 25. 273(1):97-104.

Type

Empty backbone

Expression

Bacterial

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pSC101_TIMER

Plasmid

#103057

Purpose

low copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

Depositor

Dirk Bumann

Article

Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

Insert

TIMER-bac

Expression

Bacterial

Mutation

mutations: S4T and S179T

Promoter

constitutive ybaJp promoter

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pNCS YFP

Plasmid

#91759

Purpose

Bacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)

Depositor

Erik Rodriguez, Roger Tsien

Article

Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

Insert

Venus YFP (A206K)

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET-30-His6-SUMO-AEY-CfaN

Plasmid

#122949

Purpose

Bacterial expression of N-intein

Depositor

Thomas Muir

Article

Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

Insert

CfaN

Expression

Bacterial

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-152

Plasmid

#118566

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSW031

Plasmid

#231144

Purpose

Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.

Depositor

Philip Cole

Article

Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021

Insert

S. aureus Sortase A mutant

Tags

Nostoc punctiform split intein C-terminal fragmen…

Expression

Bacterial

Mutation

P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…

Promoter

T7

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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