34,232 results

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pJMP1354

Plasmid

#119275

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Publication

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pTn7xTS-dTomato

Plasmid

#117391

Purpose

Tn7 tagging vector pTn7xTS with dTomato expression scaffold

Depositor

Karen Guillemin

Publication

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Insert

dTomato

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMRE-Tn5-150

Plasmid

#118532

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-153

Plasmid

#118535

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE137

Plasmid

#118491

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Expression

Bacterial

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pF709-pET28a-Hs-FL-ETFbeta-NHis wt

Plasmid

#85110

Purpose

expression of recombinant human full-length ETFbeta wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Publication

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human full-length ETFbeta (ETFB Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pSG22-pBAD-TetR

Plasmid

#102451

Purpose

Expresses TetR protein in E. coli. The expression is controlled by a pBAD promoter.

Depositor

Richard Murray

Publication

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

TetR (tetR )

Expression

Bacterial

Promoter

pBAD

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-157

Plasmid

#118571

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE141

Plasmid

#118493

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pDC191

Plasmid

#115792

Purpose

Expresses Rat synaptobrevin 2

Depositor

Axel Brunger

Publication

Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

Insert

Synaptobrevin2 (Vamp2 Rat)

Tags

hexa histidine

Expression

Bacterial

Mutation

S28C and C103S (please see comments below)

Promoter

T7

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNP1 sfGFP trigger

Plasmid

#107359

Purpose

Trigger sequence for pNP1 sfGFP toehold switch

Depositor

James Collins

Publication

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold trigger 7

Expression

Bacterial

Promoter

T7

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCOLA banana sensor sfGFP

Plasmid

#107367

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA

Depositor

James Collins

Publication

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

banana-detecting toehold + sfGFP

Expression

Bacterial

Promoter

T7

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pDAI-SceI

Plasmid

#32061

Purpose

Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

Depositor

Miguel Valvano

Publication

Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

Insert

I-SceI nuclease

Expression

Bacterial

Promoter

Pdhfr

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI

Plasmid

#115208

Purpose

Expresses PinCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Publication

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PinCas13

Tags

SUMO

Expression

Bacterial

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pC0062 RanCas13 (B06) His6-TwinStrep-SUMO-BsaI

Plasmid

#115213

Purpose

Expresses RanCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Publication

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

RanCas13

Tags

SUMO

Expression

Bacterial

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)_NADP-Snifit

Plasmid

#113913

Purpose

Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+

Depositor

Kai Johnsson

Publication

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Bacterial

Promoter

T7

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pHAT2

Plasmid

#112583

Purpose

Used for expression of protein in E. coli as non-cleavable minimal N-terminal 6His-tag fusions

Depositor

Marko Hyvönen, Johan Peränen

Publication

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Tags

6His

Expression

Bacterial

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJB01

Plasmid

#111957

Purpose

Expresses His10-RFP-Halo; construct generated for pull-down and localization experiments

Depositor

Matthew Good

Publication

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

RFP

Tags

HaloTag

Expression

Bacterial

Promoter

T7

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pScaf-1512.1

Plasmid

#111402

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 1512 bases in length

Depositor

Shawn Douglas

Publication

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-1512

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1-mKalama1

Plasmid

#102639

Purpose

In vitro expression of mKalama1 from the T7 promoter

Depositor

Michael Jewett

Publication

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mKalama1

Expression

Bacterial

Promoter

T7

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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