32,417 results
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Plasmid#137077PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInsertmKOκ DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPM2213
Plasmid#167553PurposeEncodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12DepositorInsertrfb region of Shigella flexneri serotype 2a
ExpressionBacterialAvailable SinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
CYP2C9 in vector pCW ori+
Plasmid#69554PurposeModification of CYP2C9 and 2C19 s for bacterial expressionDepositorInsertCYP2C9 (CYP2C9 Human)
ExpressionBacterialAvailable SinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET21-pelB-LaG94-5
Plasmid#228965PurposeFor bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaG94-5
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_PflaB-mCherryBb
Plasmid#118243PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCherryDepositorInsertflagellin promoter driven mCherry
ExpressionBacterialAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-16-3xFLAG-LaG-2
Plasmid#172765PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (3xFLAG linker)DepositorInsertLaG-16-3xFLAG-LaG-2
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
MARS_pNIC-CTB10H
Plasmid#153053PurposeExpression construct for producing Avi tagged Methionyl-tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD_TypeIC_Cascade_cas3_invivo
Plasmid#196400Purposeexpresses CRISPR Cascade-Cas3 Type IC proteins for in vivo assayDepositorInsertsCas5c
Cas7c
Cas8c
Cas3c
Tags6xHisExpressionBacterialPromoteraraBADAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM6-G6-vioABE
Plasmid#73438PurposeProdeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.DepositorInsertPG6-vioABE
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPG6 (orthogonal T7-lac variant)Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
Fusion of Zika NS2B-NS3 proteins
Plasmid#204815PurposeBacterial expression of a fusion of Zika NS2B-NS3 proteinsDepositorInsertZika NS2B-NS3 fusion (orf1ab Zika)
ExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
O-TREM2-2
Plasmid#166545PurposescFv of a human scaffold targeting Triggering receptor expressed on myeloid cells 2. Antigen coverage aa 19-131 of 230DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Gm-GW
Plasmid#64961Purposemini-Tn7 Gateway destination vectorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pKK233-hCaM
Plasmid#47598DepositorInsertCaM (CALM3 Human)
ExpressionBacterialMutationcontains 469 bp fragment (nt 92-559)PromotertrcAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSCB-YFP
Plasmid#101737PurposeThe plasmid can be either plasmid propogation backbone or expression vector in specific strainDepositorTypeEmpty backboneUseSynthetic BiologyTagseYFPExpressionBacterialPromoterTrc1OAvailable SinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
Traptavidin-E6
Plasmid#59549PurposepET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatographyDepositorInsertCore Traptavidin-E6
TagsE6 tagExpressionBacterialPromoterT7Available SinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSiMPls_N + pSiMPls_C
Plasmid#167203PurposeSiMPl plasmid pair (pSiMPls) for use with spectinomycin/streptomycinDepositorInsertsEGFP
SmR 1-75 + gp41-1 N-intein
mRuby3
gp41-1 C-intein + SmR 76-263
ExpressionBacterialPromoterAmpR, CAT, pBAD, and pTrcAvailable SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX Src-SH2
Plasmid#46510DepositorInsertSrc (SRC Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 142-252PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
His-DNPH1
Plasmid#203224PurposeFor protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only