32,926 results

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pBabe-tetoff-mVenus-PICdc42

Plasmid

#91876

Purpose

PI(WT): Photo inhibitable (blue light sensitive)

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Insert

Vav2 (VAV2 Human)

Tags

mVenus

Expression

Bacterial and Mammalian

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_A321V

Plasmid

#98671

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with A321V

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Tags

6xHis

Expression

Bacterial

Mutation

A321V

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R1079A_K1080A

Plasmid

#91861

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) R0179A/K1080A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

K1080A

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAM5087

Plasmid

#85123

Purpose

Ptrc-YFP subcloned from pAM5080 into NSII-Km vector.

Depositor

Susan Golden

Article

Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

Insert

YFP

Expression

Bacterial

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pFosill-1

Plasmid

#89695

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSY14-ptb

Plasmid

#90490

Purpose

pSY8 derivative with sceC gene under the control of ptb promoter used for I-SceI-mediated scarless gene modification via allelic exchange in Clostridium

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Insert

sceC

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4(101-437)-Hcp1

Plasmid

#87737

Purpose

Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Tags

6xHis-V5-TEV and Hcp1

Expression

Bacterial

Mutation

deleted amino acids 1-100

Promoter

T7

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-PhoCl-MBP

Plasmid

#87689

Purpose

Express PhoCl-MBP in E.coli

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PhoCl fused to Maltose-Binding Protein

Expression

Bacterial

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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Sperm whale Mb 64Y68F in pVP80K

Plasmid

#86956

Purpose

Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).

Depositor

John Olson

Insert

Mb 64Y68F

Expression

Bacterial

Mutation

64Y68F

Promoter

T5

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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OeNL_pRSETb

Plasmid

#89529

Purpose

Orange color variant of bright luminescent protein for bacterial expression

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Tags

His X 6

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-(pos14)GFP

Plasmid

#89252

Purpose

Expresses GFP with +14 theoretical charge in E.Coli

Depositor

David Liu

Article

Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

Insert

His6-(pos16)-GFP

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pET16b-Antp

Plasmid

#86866

Purpose

Bacterial expression of fusion protein with cell penetrating peptide, antennapedia fragment, at C-terminus.

Depositor

Michael Chin

Article

Chien et al Genesis. 2014 Jul;52(7):695-701. doi: 10

Insert

Linker-Antp

Tags

Antp

Expression

Bacterial

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET tdTomato LIC cloning vector (H6-tdTomato)

Plasmid

#86928

Purpose

pET LIC cloning vector for Biotin-His6-tev-yORF-tdTomato

Depositor

Chris Jeans

Type

Empty backbone

Tags

6xHis, AviTag (biotin), TEV cleavage site, and td…

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pTGD

Plasmid

#88894

Purpose

Tandem gene duplication cassette for RecA mediated homologous recombination in E. coli

Depositor

Keith Tyo

Article

Love et al Methods Mol Biol. 2019;1927:37-45. doi:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP2

Plasmid

#80658

Purpose

Encodes the synthetic coiled coil peptide SYNZIP2

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP2

Tags

6xHis

Expression

Bacterial

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pSHS212

Plasmid

#87346

Purpose

Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/C574S

Promoter

T7

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET11a-CA(A14C, E45C)

Plasmid

#79045

Purpose

Bacterial vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

CA (A14C, E45C)

Expression

Bacterial

Mutation

A14C and E45C mutations in HIV-1 CA protein

Promoter

T7

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pE-SUMO CRYGS

Plasmid

#80753

Purpose

Expression of human gamma S-crystallin in E. coli

Depositor

Larry David

Article

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYGS (CRYGS Human)

Tags

6xHIS and Smt3 SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAP84

Plasmid

#86554

Purpose

Expresses sLucopt luciferase from the constitive Bacillus subtilis promoter Pveg in Clostridium difficile

Depositor

Wiep Klaas Smits

Insert

Pveg-sLucopt

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Promoter

Pveg

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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EPB104

Plasmid

#68369

Purpose

contains human U6 promoter

Depositor

Eric-Paul Bennett

Article

Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

Insert

U6 promoter (RNU6-1 Human)

Expression

Bacterial

Promoter

U6

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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