31,059 results
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Plasmid#127151PurposeExpresses N-terminal cysteine Abeta(1-42)DepositorAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only
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pJV0047
Plasmid#205139PurposeExpression of 6xHis-EGFP-2xPX in bacteriaDepositorInserttandem 2xPX domain of SnxA
Tags6xHisExpressionBacterialAvailable SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_HCK-1/1
Plasmid#91273PurposeProtein expression and purification of human SH3 domain construct HCK-1/1DepositorInsertHCK-1/1 (HCK Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ec dnaN-pHK/P
Plasmid#237239Purposeover-express E. coli hk/P-dnaN (Pol III beta sliding clamp subunit, N-terminal cleveable his-kinase tag)DepositorInsertdnaN (beta), N-terminal his-kinase cleavable tag (dnaN E. coli)
Tagshis-10+PKA kinase motif+PreScission Protease clea…ExpressionBacterialAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-TEV-HaloTag-linker-SpyCatcher3
Plasmid#235150PurposeBacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.DepositorInsertHaloTag linked to SpyCatcher3
TagsHisExpressionBacterialPromoterT7Available SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBWB514
Plasmid#209326Purposep15A-tetR-tet-SapI-ME-RBS-mRFP-ME-U1-Barcode-U2-SapI-dbI terminator-oriT-Cm. Fragment expression vector with Golden Gate sites.DepositorInsertmRFP
ExpressionBacterialPromotertetAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL2319
Plasmid#195697PurposeCryo-EM of 6xHis-Foldon-(Gly-Ser-Linker)-MlaCDepositorInsertFoldon-MlaC
ExpressionBacterialMutation6xHis-Foldon-(Gly-Ser-Linker)-MlaCAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 aph(9)-Ia
Plasmid#112882PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertaph(9)-Ia
Tags6xHisExpressionBacterialPromoterPblaAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-DDX4LCD
Plasmid#204408PurposeE. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tagDepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS23·Pm-GFP
Plasmid#122593PurposeExpresses GFP under the regulatory control of Pm. Expression needs additional xyls geneDepositorInsertPm promoter
UseSynthetic BiologyExpressionBacterialPromoterPmAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJB02
Plasmid#111959PurposeExpresses GST-GFP-DHFR; construct generated for pull-down and localization experimentsDepositorInsertsmEGFP
eDHFR
TagsDHFRExpressionBacterialMutationeDHFR;residues 2-159 and enhanced GFP with monome…PromoterT7Available SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBBR1_DVK_AE(J23100-RiboJ-UTR1-E0040m-B0015)
Plasmid#197662PurposeExpresses GFP under the control of J23100 and RiboJ-UTR1DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-Strep2
Plasmid#119758PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Strep-II tag.DepositorTypeEmpty backboneTagsGST and Strep-tag II fusion tagExpressionBacterialPromotertac promoterAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCC25
Plasmid#177319PurposeExpression plasmid for production of recombinant T. terrestris CutA NTase fragment in E. coliDepositorInsertTtCutA 240-603
Tags6xHis-SUMOTagExpressionBacterialPromoterT7Available SinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHis17_ParM _1
Plasmid#78201PurposeExpression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensorDepositorInsertparM (K33A I27C T174A T175N C287A)
TagsHis6ExpressionBacterialMutationK33A I27C T174A T175N C287APromoterunknownAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET mCerulean LIC cloning vector (H6-mCerulean)
Plasmid#29726DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), TEV cleavage site, and mC…ExpressionBacterialAvailable SinceNov. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pACRISPR-CM
Plasmid#203588PurposeA sgRNA expression plasmid for genome editing in BurkholderiaDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDL52 (clone45)
Plasmid#188578PurposeHigh-yield production of coenzyme F420 in E. coliDepositorInsertsribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE
ExpressionBacterialMutationCodon optimization, synthetic ribosome binding si…PromoterT7 variantsAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG018 (AVAST type 2)
Plasmid#157896PurposeExpresses the AVAST type 2 defense systemDepositorInsertAVAST type 2 (STAND ATPase with uncharacterized helical N-terminal domain)
ExpressionBacterialAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Avitag-SpyCatcher002
Plasmid#102828PurposeBacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylationDepositorInsertAvitag-SpyCatcher002
TagsAvitag, GSSGS, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only