31,065 results
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Plasmid#120879PurposeExpresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.DepositorInsertCas12g1
UseCRISPRTagsmH6ExpressionBacterialPromoterlacAvailable SinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RILP
Plasmid#74600Purposeused for expression in E. coli and purificationDepositorInsertRab-interacting lysosomal protein (RILP Human)
TagsFLAG, StrepII, and superfolder GFPExpressionBacterialPromoterT7Available SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-null-GFPLVA
Plasmid#23338DepositorInsertGFP(LVA)
TagsGFP(LVA)ExpressionBacterialAvailable SinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
PtxS1-c003
Plasmid#173076PurposeExpression of recombinant fusion protein in E. coliDepositorInsertPtxS1
ExpressionBacterialMutationD35-I221Available SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXRM15
Plasmid#63150PurposeMet15 marker pXR vector uses for both plasmid assembly in vivo, as well as targeted genomic integration.DepositorTypeEmpty backboneUseCre/LoxExpressionBacterial and YeastAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTU1-B-SP44s-GUS
Plasmid#172430PurposeExpresses Streptomyces codon-optimised beta-D-glucuronidaseDepositorInsertbeta-D-glucuronidase
UseSynthetic BiologyTagsNoneExpressionBacterialPromoterSP44sAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME Integrin beta 1b
Plasmid#105976Purposeentry clone for gateway cloning, Full length Integrin beta 1b without stopDepositorInsertIntegrinbeta1b
ExpressionBacterialAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHN767
Plasmid#178884PurposeExpression of bacterial PNGaseDepositorInsertPeptide:N-glycosidase F
TagsGST, His8ExpressionBacterialPromoterT7Available SinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28CLY2
Plasmid#21761DepositorInsertECFP -EYFP linked by flexible linker containing two SGGSGG repeats
TagsHisExpressionBacterialAvailable SinceNov. 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-E988K
Plasmid#173945PurposeBacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)DepositorAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET32a-DEST
Plasmid#84652PurposeE. coli expression vector derived from pET32 with Gateway cassette.DepositorTypeEmpty backboneTagsHis, S tag, and Trx TagExpressionBacterialPromoterT7Available SinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZE21/UBP1/ClpS_V65I
Plasmid#98567PurposeExpresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operonDepositorInsertsTruncated ubiquitin cleavase, codon optimized
Mutated ATP-dependent Clp protease adapter protein
ExpressionBacterialMutationContains the V65I mutation that increases discrim…Available SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL4164 (pEffector_IscB,ωRNA)
Plasmid#210874PurposeExpresses GstIscB and native ωRNA from constitutive promotersDepositorInsertGstIscB
ExpressionBacterialAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBRG2
Plasmid#149474PurposeBroad-Host range expression vector, pBBR origin, RhaR-RhaS activator, PrhaBAD promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertRhaS-RhaR-GFP
UseSynthetic BiologyExpressionBacterialPromoterPrhaBADAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
AK2
Plasmid#38829PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNR230
Plasmid#79546PurposeBxbI attB-attP. GFP will express if sites recombine.DepositorInsertGFPmut3b
ExpressionBacterialAvailable SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSECRETS-A
Plasmid#196986PurposeFor SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterpltetO1Available SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3-AD011
Plasmid#116852PurposeExpresses araC constitutively and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD011DepositorInsertsAraC
mScarletI
UseSynthetic BiologyExpressionBacterialPromoterpBADAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-araC
Plasmid#62563PurposeArabinose biosensor. High copy plasmid that expresses GFP proportional to arabinose concentration.DepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCARS1
Plasmid#174868PurposeContains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1DepositorInsertARS1
UseSynthetic BiologyExpressionBacterial and YeastAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only