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Plasmid#124671PurposeExpresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coilDepositorInsertSpyCatcher002-oHept
UseTagsC-tag, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDF-mTET1
Plasmid#81052Purposebacterial expression of murine TET1DepositorInsertTET1 (Tet1 Mouse)
UseTags6HISExpressionBacterialMutationPromoterT7Available sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
p44K
Plasmid#45800DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHPSE-P6
Plasmid#185907PurposeEngineered Human Heparanase mutant for e.coli expressionDepositorUseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-cAbl
Plasmid#214234PurposeBacterial expression of the kinase domain of cAbl with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screeningsDepositorInsertABL1 Kinase domain (ABL1 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_nocharge
Plasmid#139118Purposeexpress hnRNPA2 LC with no charged residuesDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationall charged residues are changed to S/Q except fo…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-FF
Plasmid#13480DepositorTypeEmpty backboneUseTagsGal11PExpressionBacterialMutationPromoterAvailable sinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEMABC
Plasmid#45398DepositorUseTagsExpressionBacterialMutationPromoterNone and T7Available sinceJune 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHN1_lacUV5-mNG-PDT
Plasmid#165583PurposeExpresses mNeonGreen and C-terminally fused PDTDepositorInsertmNeonGreen-PDT
UseTagsProtein degradation tagExpressionBacterialMutationPromoterPtrcAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21c-eIF4A
Plasmid#37230DepositorInsertTIF1 (TIF1 Budding Yeast)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-TAG
Plasmid#26771DepositorInsertCAG-TdTomato-2A-H2BGFP-SV40pA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJL1-CRM197-4xDQNAT
Plasmid#128395PurposeIn vitro expression of CRM197 with C terminal 4xDQNAT glycosylation sitesDepositorInsertDiphtheria toxin
UseTags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationInactivating G52E mutationPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pelB-MBP-P66-ss
Plasmid#137045PurposeE. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavageDepositorInsertAAC66949.1 (p66 Borrelia burgdorferi B31)
UseTagsPelB signal peptide + His10 + maltose binding pro…ExpressionBacterialMutationmature P66: lacks the P66 signal peptidePromoterT7lacAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIT5_SL
Plasmid#173769PurposeBacterial genomic integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CL7-LbCas12aD156R
Plasmid#164659PurposeBacterial expression of mutated LbCas12aD156R using CL7/Im7DepositorInsertLbCas12a-D156R
UseTagsThioredoxin, CL7, SUMOExpressionBacterialMutationchanged Aspartic Acid 156 to ArgininePromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
LB1353 (Bcl-2 promoter, no IgH enh)
Plasmid#15393DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterial and MammalianMutationBcl-2 with full promoter and no IgH enhancers MHS…PromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
human p53-(1-393)-L344P
Plasmid#24862DepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationLeucine 344 mutated to ProlinePromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRBBm59
Plasmid#48115PurposeShuttle vector E. coli/B.meg.DepositorInsertpromoter sacB - gfp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
p404GAL1
Plasmid#17417DepositorInsertGAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGAL1 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only