30,808 results

1. pGEX-gp78c/2767

   Plasmid

   #11429

   Depositor

   Allan Weissman

   Article

   Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

   Insert

   gp78c (AMFR Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   n/a

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pDEST-APLO

   Plasmid

   #112805

   Purpose

   gateway cloning destination vector to express LexAop2 driven transgene in Drosophila

   Depositor

   Chun Han

   Article

   Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. p15A-OptoCre-knt-P**-R

   Plasmid

   #189851

   Purpose

   Cre-inducible activation of knt for kanamycin resistance.

   Depositor

   Mary Dunlop

   Article

   Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

   Insert

   P**-loxP-TT-loxP-R-knt
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P** (modified T7 phage gene 10)

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p15A-OptoCre-cat-P-R

   Plasmid

   #189853

   Purpose

   Cre-inducible activation of cat for chloramphenicol resistance.

   Depositor

   Mary Dunlop

   Article

   Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

   Insert

   P-loxP-TT-loxP-R-cat
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Bacterial

   Promoter

   P (T7 phage gene 10)

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBbB8k-csg-nb112

   Plasmid

   #171702

   Purpose

   Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the spike protein of SARS-CoV-2 via a flexible linker.

   Depositor

   Neel Joshi

   Article

   Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1

   Insert

   csgBACEFG, with nanobody fused to CsgA
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGST-Sp1

   Plasmid

   #27264

   Depositor

   Thomas Gilmore

   Article

   Sif et al J Virol. 1994 Nov . 68(11):7131-8.

   Insert

   Sp1 (SP1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 83-621

   Available Since

   Feb. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

7. PZac2.1 hSyn1-Lck-GFP

   Plasmid

   #177333

   Purpose

   Expresses GFP on the plasma membrane of neurons via an Lck localization sequence

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   Lck-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Bacterial

   Promoter

   hSyn1

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAM552

   Plasmid

   #154131

   Purpose

   Expresses wt rRNAs operon in bacterial cells

   Depositor

   Alexander Mankin

   Article

   Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

   Insert

   wt bacterial rRNAs operon under control of pL promoter
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTXB1-Tn5-F

   Plasmid

   #160081

   Purpose

   Bacterial expression of C-terminally FLAG-tagged Tn5 transposase

   Depositor

   Pier Pandolfi

   Article

   Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

   Insert

   Tn5 transposase
   

   Tags

   FLAG and Mxe intein - Chitin-binding domain

   Expression

   Bacterial

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBBR5pemIK

    Plasmid

    #126554

    Purpose

    Broad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection

    Depositor

    Lindsey Burbank, Drake Stenger

    Article

    Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET51b_HCAII

    Plasmid

    #187110

    Purpose

    Expression of HCAII in bacteria

    Depositor

    Kai Johnsson

    Article

    Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

    Insert

    HCAII
    

    Tags

    Hisx10 and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZS47a luxI-laa RBS

    Plasmid

    #48892

    Purpose

    ArsR-regulated expression of luxI-laa

    Depositor

    Jeff Hasty

    Article

    Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

    Insert

    LuxI
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pArsR

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSF-DUET1-DHDDS/Sumo-NgBR

    Plasmid

    #203162

    Purpose

    Expression of human cis-prenyltransferase complex for purification

    Depositor

    William Sessa

    Article

    Edani et al Proc Natl Acad Sci U S A. 2020 Aug 25;11

    Inserts

    DHDDS (DHDDS Human)
    NUS1 dehydrodolichyl diphosphate synthase subunit (NUS1 Human)
    

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    truncation

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3xNLS-NLP-cMyc-cMyc LbaCas12a

    Plasmid

    #182125

    Purpose

    pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    3xNLS-NLP-cMyc-cMyc LbaCas12a
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ubiquitin K48C

    Plasmid

    #86588

    Purpose

    Bacterial expression of Ubiquitin K48C for cross linking studies

    Depositor

    Sharon Rozovsky

    Article

    Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

    Insert

    Ubiquitin (UBC Human)
    

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    TEV site; K48C

    Promoter

    Tac

    Available Since

    Feb. 24, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. RAB1A

    Plasmid

    #25521

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB1A (RAB1A Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pACλCI

    Plasmid

    #53730

    Purpose

    PlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    bacteriophage lambda CI protein
    

    Expression

    Bacterial

    Promoter

    lac UV5

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHH0103_BTK-1/1

    Plasmid

    #91350

    Purpose

    Protein expression and purification of human SH3 domain construct BTK-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    BTK-1/1 (BTK Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pE1n

    Plasmid

    #17441

    Purpose

    modified pENTR vector for N-terminal STREP tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    STREP

    Expression

    Bacterial, Insect, Mammalia…

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAWP78-PVCpnf_FLAG-pvc2

    Plasmid

    #198285

    Purpose

    Contains an external epitope tag on the sheath protein; for immunofluorescence-based binding assays

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_FLAG-pvc2
    

    Expression

    Bacterial

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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