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VRK1_HUMAN_D0

Plasmid

#79684

Purpose

This plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

VRK1 (VRK1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pGEX-KG-WASP-GBD

Plasmid

#30113

Depositor

David Sacks

Article

Kim et al J Biol Chem. 2000 Nov 24. 275(47):36999-

Insert

WASP-GBD (WAS, WAS Human)

Tags

GST

Expression

Bacterial

Mutation

Contains the Cdc42-interacting domain of WASP (aa…

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pEvol-MmKlacRS1

Plasmid

#232157

Purpose

Site-specific incorporation of L-lactyl-lysine into target proteins in E. coli cells.

Depositor

Nanxi Wang

Article

Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

Insert

KlacRS1

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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FLuc-N-HiBiT

Plasmid

#207117

Purpose

Bacterially expressed HiBiT-FLuc for Ni-NTA purification

Depositor

James Inglese

Article

Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

Insert

luciferin 4-monooxygenase

Tags

HiBiT Tag and His-tag with thrombin site

Expression

Bacterial

Mutation

Codon optmization for expression in BL21DE3

Promoter

T7 Promotor

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTn7xTS_SpyCatcher:mKate2

Plasmid

#225188

Purpose

Used for inserting SpyCatcher fused to mKate2 at the bacterial Tn7 site

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

SpyCatcher:mKate2

Tags

SpyCatcher

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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VAMP8

Plasmid

#186689

Purpose

Expresses VAMP8 in E. Coli

Depositor

Axel Brunger

Article

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

VAMP8

Tags

3xFLAG, GST, TEV, and Thrombin

Expression

Bacterial

Promoter

T7

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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20 pCOLI_A_N-His_C_STREP

Plasmid

#160162

Purpose

N-term 6xHis 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vector

Depositor

John Weir

Article

Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

Type

Empty backbone

Tags

2xStrepII and 6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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PZac2.1 hSyn1-Lck-13xLinker-BioID2-BioID2-HA

Plasmid

#176854

Purpose

Expresses membrane bound biotin ligase, BioID2, in neurons

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Lck-13xLinker-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

hSyn1

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCBFD2Bad

Plasmid

#53574

Purpose

Produces an active CBFD (carotenoid β-ring 4-dehydrogenase) enzyme in E. coli when expression is induced with arabinose

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 2011 Aug;23(8):3055-69. doi:

Insert

cbfd2

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-5X1 Flag PA1

Plasmid

#15560

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

PA1 (PAGR1 Human)

Tags

Flag and GST

Expression

Bacterial

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
AcrIIA2

Plasmid

#101042

Purpose

Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

Depositor

Zhiwei Huang

Article

Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

Insert

AcrIIA2

Tags

GST

Expression

Bacterial

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMyCA

Plasmid

#84268

Purpose

A versatile vector for recombinant mycobacterial protein production

Depositor

Young-Hwa Song

Article

Magana Vergara et al Protein Sci. 2017 Aug 31. doi: 10.1002/p

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

Aceteamidase

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pJS61

Plasmid

#207585

Purpose

GFP-TolA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TolA

Expression

Bacterial

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pQLinkHD

Plasmid

#13668

Depositor

Konrad Buessow

Article

Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

Insert

Gateway cassette

Use

Co-expression

Tags

His

Expression

Bacterial

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI

Plasmid

#115208

Purpose

Expresses PinCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PinCas13

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCPP6534

Plasmid

#128735

Purpose

Gateway destination pUC18R6KT-Tn7T-PavrPto-GW-Cya-Km vector for integration into DC3000D36E genome glmS-linked attTn7 site any effector gene, expressed from avrPto promoter and with C-terminal HA tag

Depositor

Alan Collmer

Article

Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCoofy1-psHaloTag1a

Plasmid

#232940

Purpose

Photoswichable HaloTag: psHaloTag1a variant

Depositor

Claire Deo

Article

Walterspiel et al Angew Chem Int Ed Engl. 2025 Oct 23:e202

Insert

photoswitchable HaloTag 1a variant

Tags

His6x-HRV3C

Expression

Bacterial

Mutation

E143W, A258W with linkers L1 (FAG) and L2 (P)

Promoter

T7

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pWUKI 1+2

Plasmid

#79879

Purpose

Encodes Cas1+2 and a CRISPR array

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1
Cas2
CRISPR Array

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pET tdTomato LIC cloning vector (H6-tdTomato)

Plasmid

#86928

Purpose

pET LIC cloning vector for Biotin-His6-tev-yORF-tdTomato

Depositor

Chris Jeans

Type

Empty backbone

Tags

6xHis, AviTag (biotin), TEV cleavage site, and td…

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pP4-C

Plasmid

#225097

Purpose

gfpUV reporter vector with a high performance adenine-dependent riboswitch

Depositor

Robert Batey

Article

Drogalis et al PLoS One. 2020 Dec 1;15(12):e0243155. do

Insert

synthetic riboswitch

Expression

Bacterial

Promoter

synthetic constitutive promoter

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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