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33,449 results

Showing: 6461 - 6480 of 33449 results

  1. D. vulgaris sp2 CRISPR/pACYCDuet-1

    Plasmid
    #81186
    Purpose
    Expresses CRISPR array containing 3 copies of Desulfovibrio vulgaris spacer 2 and flanking repeats in E. coli
    Depositor
    Jennifer Doudna
    Publication
    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:
    Insert
    Synthetic CRISPR array (3 copies of D. vulgaris spacer #2 flanked by repeats)
    Use
    CRISPR
    Tags
    None
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    81186-plasmid-map-sequence-id-162820

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  2. pAV124

    Plasmid
    #63190
    Purpose
    pRS424 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63190-plasmid-map-sequence-id-104887

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  5. pNR291

    Plasmid
    #79555
    Purpose
    Output plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.
    Depositor
    Timothy Lu
    Publication
    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.
    Inserts
    GFPmut3b
    mTagBFP
    mRFP
    Expression
    Bacterial
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79555-plasmid-map-sequence-id-148766

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  6. pColdII-SIRPT1-Bubalus bubalis

    Plasmid
    #75350
    Purpose
    Nt His-tag Bubalus bubalis Sacsin 86-1364
    Depositor
    Kalle Gehring
    Insert
    Sacsin Bubalus bubalis 86-1364
    Tags
    His-Tag
    Expression
    Bacterial
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    75350-plasmid-map-sequence-id-142906

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  7. pTEV5_ RpMatB_2

    Plasmid
    #78206
    Purpose
    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter binding
    Depositor
    Martin Webb
    Publication
    Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do
    Insert
    matB (His6 C106A S170A R286C Q457C K488A)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    C106A S170A R286C Q457C K488A
    Promoter
    T7
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78206-plasmid-map-sequence-id-146045

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  9. pUCmEos3.2:cat

    Plasmid
    #78100
    Purpose
    Plasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.
    Depositor
    Peng Chen
    Publication
    Chen et al Nat Commun. 2015 Jul 6;6:7445. doi: 10.1
    Insert
    mEos3.2-cat
    Expression
    Bacterial
    Available since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. SMARCA2

    Plasmid
    #73250
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SMARCA2B (SMARCA2 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    73250-plasmid-map-sequence-id-136215

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  15. DNMT1

    Plasmid
    #25165
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    dnmt1.0351.0600 (DNMT1 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    April 4, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. pdCas9-M-3A2

    Plasmid
    #73435
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 3A2 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73435-plasmid-map-sequence-id-135340

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Showing: 6461 - 6480 of 33449 results