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pet42c(+)-GST-USP13

Plasmid

#61749

Purpose

express N-terminally GST tagged human USP13

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13 (USP13 Human)

Tags

GST

Expression

Bacterial

Promoter

T7

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
pEXP5-NT/pT7 LasI

Plasmid

#193631

Purpose

Constitutive T7 RNAP-mediated expression of LasI 3OC12-HSL synthase for Las quorum sensing system. LasI sequence is codon optimized for E. coli.

Depositor

Dora Tang

Article

Gonzales et al ChemSystemsChem 2023

Insert

LasI

Tags

6xHis

Expression

Bacterial

Promoter

T7 RNAP promoter

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pJK_kanR_proJK1_PAD

Plasmid

#60630

Purpose

Constitutively expresses PAD (phenolic acid decarboxylase)

Depositor

Timothy Whitehead

Article

Tomek et al Biotechnol Bioeng. 2015 Mar;112(3):627-3

Insert

Phenolic acid decarboxylase padC (padC )

Expression

Bacterial

Mutation

K146Y

Promoter

proJK1

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pSA1

Plasmid

#52261

Purpose

Bacterial expression of human 6His-tagged SUMO1 and SUMO-E1

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-1 (SUMO1 Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pQC

Plasmid

#48100

Purpose

Cumate-inducible expression of ECFP-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

3xFLAG and ECFP

Expression

Bacterial

Promoter

PQ5

Available Since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-VCAM1

Plasmid

#202471

Purpose

Expresses the nanobody VCAM1 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody VCAM1

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pTHSSe_39

Plasmid

#109238

Purpose

PTac driving TALEsp1

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

TALEsp1

Expression

Bacterial

Promoter

PTac

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pdCas9-omega

Plasmid

#114632

Purpose

Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

Depositor

Anushree Chatterjee

Article

Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

Insert

dCas9-omega

Use

CRISPR

Expression

Bacterial

Promoter

Tet promoter

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSF-p15A-trc- YFP

Plasmid

#186569

Purpose

p15A ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Expression

Bacterial

Promoter

trc promoter

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-TAG

Plasmid

#26771

Depositor

Shankar Srinivas

Article

Trichas et al BMC Biol. 2008 Sep 15;6:40. doi: 10.1186

Insert

CAG-TdTomato-2A-H2BGFP-SV40pA

Expression

Bacterial

Available Since

March 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRC319

Plasmid

#61272

Purpose

Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.

Depositor

Timothy Lu

Article

Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

Insert

Cas9, tracrRNA, crRNA

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pTK204

Plasmid

#13534

Depositor

Wolf Frommer

Article

Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

Insert

FLIPW-CTYT

Tags

See map

Expression

Bacterial

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only

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pMEXNH3

Plasmid

#110079

Purpose

Analogous to pMINTNH3, but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

His10 Tag - 3C cleavage site

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_VASP_WH1

Plasmid

#109790

Purpose

Protein expression and purification of VASP_WH1

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

VASP_WH1 (VASP Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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KAT2B

Plasmid

#39002

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

KAT2B (KAT2B Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
His6_MBP_FXR2P_P474S_P493N

Plasmid

#198695

Purpose

Expression of human FXR2 protein in E. coli

Depositor

Simpson Joseph

Article

Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

Insert

FXR2P (FXR2 Human)

Tags

MBP

Expression

Bacterial

Mutation

Changed proline 474 to serine and proline 493 asp…

Promoter

T7

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pNP1 sfGFP toehold switch

Plasmid

#107355

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 sfGFP trigger is co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold 7 + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pZE-PalkB-GFP

Plasmid

#44381

Depositor

Hal Alper

Article

Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. d

Inserts

GFP
PalkB

Use

Synthetic Biology

Expression

Bacterial

Promoter

PalkB

Available Since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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MBP-MEK1

Plasmid

#68300

Purpose

N-terminal MBP tagged human MEK1 gene for bacterial expression

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

MBP-MEK1 (MAP2K1 Synthetic, Human)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pAIP4_1UXZ

Plasmid

#234166

Purpose

Heterologous protein expression of cellulase B in Escherichia coli

Depositor

Christopher Bahl

Insert

1UXZ

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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