30,808 results

1. pDF0191 CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

   Plasmid

   #172505

   Purpose

   Encodes the CjcCas7-11 protein only for bacterial recombinant protein expression for purification

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNIC-NH10-GG

   Plasmid

   #215806

   Purpose

   Cloning of target gene with N terminal non cleavable His10 tag

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. Dual opposing T7 promoters reporter plasmid

   Plasmid

   #156457

   Purpose

   A mutational reporter plasmid that can gain Kanamycin resistance upon mutation. Contains two T7 promoters flanking the kanamycin resistance gene.

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

   Insert

   NeoR/KanR
   

   Expression

   Bacterial

   Mutation

   ATG start codon mutated to ACG

   Promoter

   Em7

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-RBP-LOV2

   Plasmid

   #185792

   Purpose

   Encodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linker

   Depositor

   Lizhong He

   Article

   Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

   Insert

   RBP-LOV2
   

   Tags

   10xHis, TEV protease cleavage site after His Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28b-6His-AcpS

   Plasmid

   #145376

   Purpose

   Expression of E. coli AcpS

   Depositor

   Robert Tomko

   Article

   Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

   Insert

   AcpS (acpP E. coli)
   

   Tags

   6-His

   Expression

   Bacterial

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. TDP-43_CTD_A321V

   Plasmid

   #98671

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with A321V

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A321V

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX-4T-2/hGGA3(GAT)

   Plasmid

   #79436

   Purpose

   Expresses GST-tagged GAT domain of GGA3 (107-286 aa) in E.coli

   Depositor

   Kazuhisa Nakayama

   Article

   Takatsu et al Biochem J. 2002 Jul 15;365(Pt 2):369-78.

   Insert

   GGA3 (GGA3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 107-286

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pADS

   Plasmid

   #19040

   Depositor

   Jay Keasling

   Article

   Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

   Insert

   codon-optimized synthetic amorphadiene synthase
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

9. PatoL-OG241

   Plasmid

   #72841

   Purpose

   Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.

   Depositor

   Chris Barnes

   Article

   Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

   Insert

   PatoL
   

   Expression

   Bacterial

   Promoter

   PatoL

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

    Plasmid

    #172501

    Purpose

    Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    DisCas7-11 full locus with Golden gate acceptor site for spacer
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTE-28S15-0X

    Plasmid

    #20319

    Depositor

    Dirk Schnappinger

    Article

    Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

    Insert

    Psmyc-tetR#28
    

    Expression

    Bacterial

    Available Since

    March 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBP-C_NanoLuc

    Plasmid

    #204112

    Purpose

    Type 0 CDS part

    Depositor

    Mikhail Shapiro

    Insert

    NanoLuc luciferase
    

    Expression

    Bacterial

    Mutation

    None

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCW-LIC-N8C_Fab4a

    Plasmid

    #203690

    Purpose

    Expresses N8C_Fab4a

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab4a
    

    Expression

    Bacterial

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDF_LuxR

    Plasmid

    #140039

    Purpose

    Constutitive expression of LuxR protein.

    Depositor

    Yolanda Schaerli

    Article

    Barbier et al Mol Syst Biol. 2020 Jun;16(6):e9361. doi

    Insert

    pLuxL and LuxR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pH6-TEV-mCherry

    Plasmid

    #132430

    Purpose

    Expresses His6-tagged mCherry in E. coli using T7 promoter

    Depositor

    Mark Arbing

    Insert

    mCherry
    

    Tags

    N-terminal His6 tag with TEV protease site

    Expression

    Bacterial

    Mutation

    Codon optimized

    Promoter

    T7

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-mCherryGGBsMTS

    Plasmid

    #133625

    Purpose

    Expression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherryGGBsMTS
    

    Expression

    Bacterial

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b

    Plasmid

    #122850

    Purpose

    Expresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coli

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    m(KS_MAT)_mouseL_ACP (Fasn Mouse)
    

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p11d-tRPA-32Ala9

    Plasmid

    #102616

    Purpose

    Expression of human RPA with an alanine substituted phosphorylation domain (with the following mutations in RPA32 :S8,11-13,23,29,33,39A,T21A).

    Depositor

    Marc Wold

    Inserts

    RPA1 (RPA1 Human)
    RPA3 (RPA3 )
    RPA2 (RPA2 )
    

    Expression

    Bacterial

    Mutation

    with an alanine substituted phosphorylation domain

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pATmin-GG

    Plasmid

    #191352

    Purpose

    Clostridium expression vector (pAMB1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Insert

    Plac-RFP (IPTG-inducible red fluorescent protein)
    

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX4T1-Smurf2 wt

    Plasmid

    #13504

    Depositor

    Jeff Wrana

    Article

    Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

    Insert

    SMURF2 (SMURF2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Dec. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge