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CJ8421_04975 Rptr2 and CJ8421_04975 mRNA

Plasmid

#194210

Purpose

Expresses the Campylobacter mRNA CJ8421_04975 and the mRNA-targeting reprogrammed tracrRNA 2

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

CJ8421_04975 mRNA and CJ8421_04975 Rptr2

Expression

Bacterial

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pKB236

Plasmid

#170027

Purpose

Encodes LacI-controlled expression of surface-displayed TCP and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
TCP

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

July 31, 2025

Availability

Academic Institutions and Nonprofits only

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pGI3EM11

Plasmid

#135483

Purpose

Agrobacterium T-DNA with SpunH2Bpr driving hph-GFP

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunH2Bpr hph-GFP ADH1t

Tags

GFP

Expression

Bacterial and Yeast

Promoter

SpunH2Bpr

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-T-rnVAPA-1-125

Plasmid

#13396

Depositor

Axel Brunger

Article

Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

Insert

VAP-A (Vapa Rat)

Tags

GST

Expression

Bacterial

Available Since

Feb. 17, 2009

Availability

Academic Institutions and Nonprofits only
pGBW-m4046959

Plasmid

#145722

Purpose

Bacterial Expression plasmid for SARS-CoV-2 ORF3a protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

wt

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available Since

April 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTU424

Plasmid

#178983

Purpose

pACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)

Depositor

Stan Brouns

Article

van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

Insert

TPR-CHAT

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pME Integrin beta 1b

Plasmid

#105976

Purpose

entry clone for gateway cloning, Full length Integrin beta 1b without stop

Depositor

Caren Norden

Article

Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

Insert

Integrinbeta1b

Expression

Bacterial

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pM1s3AsR_TS-C7

Plasmid

#137945

Purpose

E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive RFP expression and strong temperature sensitive GFP expression

Depositor

Neel Joshi

Article

Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology; For use with e. coli nissle in…

Expression

Bacterial

Promoter

J23101

Available Since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-Fast-FT

Plasmid

#31916

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Fast-FT

Tags

His

Expression

Bacterial

Mutation

M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

Promoter

araB

Available Since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only

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GLI K12

Plasmid

#16419

Depositor

Bert Vogelstein

Article

Kinzler et al Nature. 1988 Mar 24. 332(6162):371-4.

Insert

GLI (GLI1 Human)

Expression

Bacterial

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI

Plasmid

#115210

Purpose

Expresses AspCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

AspCas13

Tags

SUMO

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCDFNKI-strepII-3C-LIC-strep

Plasmid

#108708

Purpose

Expression of a StrepII-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

StrepII-3C protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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APneg-VAMP1d

Plasmid

#168010

Purpose

negative selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

VAMP1(T29-K89)-T7PAP

Expression

Bacterial

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pSL4164 (pEffector_IscB,ωRNA)

Plasmid

#210874

Purpose

Expresses GstIscB and native ωRNA from constitutive promoters

Depositor

Samuel H. Sternberg

Article

Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

Insert

GstIscB

Expression

Bacterial

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_3

Plasmid

#60564

Purpose

A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Reporter_3

Expression

Bacterial

Promoter

BBa_J23119

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pGal CycK (P#844)

Plasmid

#14664

Depositor

Matija Peterlin

Article

Lin et al J Biol Chem. 2002 May 10. 277(19):16873-

Insert

CycK (CCNK Human)

Expression

Bacterial

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pOKD4

Plasmid

#17214

Depositor

Hao Wu

Article

Dzivenu et al Protein Expr Purif. 2004 Nov . 38(1):1-8

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pTorPE-jREX-GECO1

Plasmid

#113941

Purpose

Bacterial expression of red fluorescent Ca2+ sensor jREX-GECO1

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

jREX-GECO1

Tags

6xHis and Modified TorA MNNNDLFQASRRRFLAQLG[G to …

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-luxKate

Plasmid

#229946

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxKate

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMAK461

Plasmid

#140699

Purpose

NBαRb-IgG-Fc

Depositor

Takayuki Iwaki

Article

Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

Insert

NBαRb-IgG-Fc

Expression

Bacterial

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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