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pGEM-LIN28

Plasmid

#16350

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens lin-28 homolog (C. elegans) (LIN28A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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Aurein1.2_(+36GFP)-Cre

Plasmid

#71748

Purpose

Aurein1.2+36GFP-Cre fusion expression in E. Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

(GGS)9_Aurein1.2+36GFP-Cre

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔM13-mCherry-PR/PRM-GFP

Plasmid

#80859

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Lambda Pr and Lambda Prm

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-153

Plasmid

#118535

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Use

Minitn5 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-23Q

Plasmid

#84349

Purpose

Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Use

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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ESCRT-II

Plasmid

#17633

Depositor

James Hurley

Article

Hierro et al Nature. 2004 Sep 9. 431(7005):221-5.

Insert

pST39-Vps25(1-203) - His/Vps22(1-234) - Vps36 (1-567) (VPS36, SNF8, VPS25 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-14-3-3 zeta GST

Plasmid

#13278

Depositor

Michael Yaffe

Article

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 zeta (YWHAZ Bovine)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only
pKB233

Plasmid

#170040

Purpose

Encodes PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Insert

mNeonGreen

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PvirK

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-ELP[AV-60]

Plasmid

#67012

Purpose

Expresses malE-ELP[AV-60] in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-ELP[AV-60]

Use

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pDD04neo pmyo2::gfp

Plasmid

#26393

Depositor

Denis Dupuy

Article

Giordano-Santini et al Nat Methods. 2010 Aug 22. ():.

Type

Empty backbone

Use

Tags

Expression

Bacterial and Worm

Mutation

Promoter

Available since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pAtetO_6xHis-v5.9

Plasmid

#155259

Purpose

Plasmid for the aTc-inducible expression of the v5.9

Depositor

Andrew Ellington

Article

Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

Insert

v5.9

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

tet PA

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#45

Plasmid

#153526

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#45

Depositor

Markus Seeger

Article

Walter et al bioRxiv 2020.04.16.045419

Insert

Sb#45

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pgex6p-1mciap1

Plasmid

#11465

Depositor

Jon Ashwell

Article

Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56.

Insert

mciap1 (Birc2 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
pCSW21

Plasmid

#86836

Purpose

bacterial expression of anti-CRISPR protein AcrIIA4

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA4

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Stu2 1-306

Plasmid

#38315

Depositor

Anthony Hyman

Article

Widlund et al Mol Biol Cell. 2012 Sep 19.

Insert

5' 918 bp from STU2 ORF (STU2 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pET 2C-T10-dCasX

Plasmid

#126418

Purpose

E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

Depositor

Jennifer Doudna

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

DpbCasX

Use

CRISPR

Tags

His-MBP

Expression

Bacterial

Mutation

Promoter

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCNA

Plasmid

#135191

Purpose

Arabinose inducible expression of lamba-Red recombinase proteins and I-CreI.

Depositor

Hongqing Fang

Article

Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

Insert

I-CreI lamba Red

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

araBAD

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2832

Plasmid

#160672

Purpose

Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

gmc6 (gfp) sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2820

Plasmid

#160669

Purpose

Mobile CRISPRi sfGFP 'test', empty sgRNA

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Insert

empty sgRNA (BsaI)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pT7C-DNAJB6

Plasmid

#177657

Purpose

Recombinant protein synthesis of human DNAJB6

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

DNAJB6 (DNAJB6 Human)

Use

Tags

6xHis-Thrombin

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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