32,934 results
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Plasmid#154397PurposeDerived from pGPI-SceI; Trimethoprim resistant; carries I-SceI gene; contains 475 bp from both upstream and downstream flanking regions between the FRT sites on pAHCTX1-rhadCas9DepositorInsertFused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome
UseSynthetic BiologyExpressionBacterialPromoterNAAvailable SinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28b-XI
Plasmid#157988PurposeExpresses XI in E.coli cellDepositorInsertxylose isomerase
ExpressionBacterialAvailable SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AT110.i1
Plasmid#135481PurposeE. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptorDepositorInsertNanobody AT110.i1
Tags6xHis-tag and pelB leaderExpressionBacterialPromoterT7Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-DUET-1-mRhubarb719-HO
Plasmid#141200PurposeExpresses mRhubarb719 fluorescent protein and heme oxygenaseDepositorInsertsmRhubarb719
heme oxygenase
Tags6x-HisExpressionBacterialMutationR134H, Y198S, T202Y, V203I, W309R, Q310A compared…PromoterT7Available SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHis-LMW_FGF2
Plasmid#157657PurposeExpresses FGF2 in E.coli cellDepositorInsertFibroblast growth factor 2 (FGF2 Human)
TagsHis tagExpressionBacterialMutationchanged Cystein 211,229 to serine, deleted amino …Available SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
mUAVbla:tetR 5′
Plasmid#149379PurposemUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangsDepositorInserttetA
UseSynthetic BiologyExpressionBacterialAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+mEGFP-H1.4
Plasmid#157799PurposeExpression of mEGFP and human linker histone H1.4 fusion proteinDepositorAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ2018
Plasmid#140665PurposedCas9, LuxR & Csy4DepositorInsertdCas9, LuxR & Csy4
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQadd2
Plasmid#135662PurposeClostridial vector, carrying lox511/66 and loxFAS/71 sites separated by restriction sites in order to add cargo. Use in combination with pQcre1 to insert cargo into lox sites, inserted with pQadd1F/R.DepositorInsertLox511/66 and LoxFas/71
UseClostridial vector, pamβ1 originExpressionBacterialAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ2048.2
Plasmid#140687Purpose3-color stripe (different sgRNAs)DepositorInsert3-color stripe (different sgRNAs)
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL727
Plasmid#140397PurposeExpresses CsoR protein under the control of T7-lacO promoterDepositorInsertT7-lacO-CsoR-T
Tags6XHis-TEVExpressionBacterialAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
MARS_pNIC-CTB10H
Plasmid#153053PurposeExpression construct for producing Avi tagged Methionyl-tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
AARS_C2_pNIC-Bio3
Plasmid#153047PurposeExpression construct for producing Avi tagged Alanyl-tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL0M-S-tdTomato-EC18158
Plasmid#137078PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInserttdTomato DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS*-PrpoN-CroAct3 63aa-PBBa_J23106-geneIII
Plasmid#134355PurposePhagemidDepositorInsertCroAct3 63aa
ExpressionBacterialAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJPC12-ΔPS-mCherry-PCS/PM,CS-GFP
Plasmid#134357PurposeReporter plasmidDepositorInsertsGFP
mCherry
ExpressionBacterialAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAK463
Plasmid#140701PurposeNBαMs-IgKDepositorInsertNBαMs-IgK
ExpressionBacterialAvailable SinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MBP-His-ACPs
Plasmid#141140PurposeExpression of holo-[acyl-carrier-protein] synthaseDepositorInsertacpS
TagsMBPExpressionBacterialAvailable SinceMay 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGI3EM18
Plasmid#135484PurposeAgrobacterium T-DNA with SpunH2Bpr driving hph-tdTomatoDepositorInsertSpunH2Bpr hph-tdTomato ADH1t
TagstdTomatoExpressionBacterial and YeastPromoterSpunH2BprAvailable SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM22C
Plasmid#135488PurposeAgrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hphDepositorInserthph SpunH2A/Bpr LifeAct-tdTomato
TagsLifeActExpressionBacterial and YeastPromoterSpunH2A/Bpr (divergent)Available SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only