30,808 results

1. 6xHis-MBP-huPb2Cas12a

   Plasmid

   #174686

   Purpose

   Expresses 6xHis-MBP tagged Pb2Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huPb2Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSG25-pBAD-tetO-deGFPssrA

   Plasmid

   #102452

   Purpose

   Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.

   Depositor

   Richard Murray

   Article

   Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

   Insert

   deGFP
   

   Tags

   ssrA

   Expression

   Bacterial

   Promoter

   pBAD-tetO

   Available Since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAOJ15

   Plasmid

   #91567

   Purpose

   Broad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoter

   Depositor

   Harry Mobley

   Article

   Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   Tetracycline promoter

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTETDuet_M42FG121VDHFR_R166QTS

   Plasmid

   #191298

   Purpose

   Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

   Depositor

   Kimberly Reynolds

   Article

   Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

   Insert

   folA, thyA
   

   Expression

   Bacterial

   Mutation

   M42F/G121V DHFR, R166Q TYMS

   Promoter

   T7 (folA), Tet (thyA)

   Available Since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PZac2.1 hSyn1-HA-BioID2-SAPAP3

   Plasmid

   #176857

   Purpose

   Expresses SAPAP3 protein fused with BioID2 at the N-terminus in neurons

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   HA-BioID2-SAPAP3
   

   Use

   AAV

   Tags

   HA

   Expression

   Bacterial

   Promoter

   hSyn1

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJL1-mTagBFP2

   Plasmid

   #102638

   Purpose

   In vitro expression of mTagBFP2 from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   mTagBFP2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28_mouse-Caspase-11_enzymatic-domain

   Plasmid

   #183382

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-11 (AA100-373) (Casp4 Mouse)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCORE-UH

   Plasmid

   #72239

   Purpose

   Contains the CORE cassette with KlURA3 and hyg markers

   Depositor

   Michael Resnick, Francesca Storici

   Article

   Storici et al Genet Eng (N Y). 2003;25:189-207.

   Inserts

   KlURA3
   hyg
   

   Expression

   Bacterial

   Available Since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-His-MinD_MinE

   Plasmid

   #133621

   Purpose

   Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.

   Depositor

   Petra Schwille

   Article

   Loose et al Science. 2008 May 9;320(5877):789-92. do

   Insert

   His-MinD_MinE (minD, minE E. coli)
   

   Expression

   Bacterial

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNS38-SadCas9-SNAP

    Plasmid

    #113718

    Purpose

    Bacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA-2xNLS-SNAP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    D10A, N580A

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQE80L-SpyCatcher-ELP-GFP

    Plasmid

    #69835

    Purpose

    Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

    Insert

    SpyCatcher-ELP-GFP
    

    Tags

    6x His Tag, GFP, and TEV Tag

    Expression

    Bacterial

    Promoter

    T5 promoter/lac operator element

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. 371-GFP

    Plasmid

    #97030

    Purpose

    His6-GFP-tev-yORF

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Tags

    GFP fusion, His6 tag, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWJ68

    Plasmid

    #46571

    Purpose

    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    w-dcas9
    CRISPR array
    

    Use

    CRISPR

    Tags

    rpoZ

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. CTHA

    Plasmid

    #42365

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CTHA (CTH Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pGEX-2T SHP1 WT

    Plasmid

    #8594

    Depositor

    Ben Neel

    Article

    Plutzky et al Proc Natl Acad Sci U S A 1992 Feb 1;89(3

    Insert

    SHP1 (PTPN6 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    June 21, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pHYRSF1

    Plasmid

    #34549

    Depositor

    Hideo Iwai

    Article

    Muona et al Nat Protoc. 2010 . 5(3):574-87.

    Insert

    DnaE N-intein
    

    Tags

    H6-GB1

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. PDLIM4_PDZ_1

    Plasmid

    #103941

    Purpose

    Protein expression and purification of RIL BGLII PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PDLIM4 (PDLIM4 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHG6

    Plasmid

    #188254

    Purpose

    Integrating plasmid contains the BT1 int/attP locus and the erythromycin-resistance gene for selection

    Depositor

    Hong Gao, Maggie Smith

    Article

    Gao et al Access Microbiol. 2021 Dec 8;3(12):00029

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    act1p/actII-orf4

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACDura3

    Plasmid

    #138711

    Purpose

    Expresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.

    Depositor

    Víctor de Lorenzo

    Article

    Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

    Insert

    ura3 gene (URA3 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    optimized ura3 gene for expression in Escherichia…

    Promoter

    pEM7 promoter

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. (SUMO)5

    Plasmid

    #126949

    Purpose

    Bacterial expression plasmid for (SUMO)5 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    5 repeats of human SUMO3 separated by (GGS)4 (SUMO3 Human)
    

    Expression

    Bacterial

    Promoter

    unknown

    Available Since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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