-
-
pBADmod1-linker2-gamS
Plasmid#45833DepositorInsertgamS
UseSynthetic BiologyTagsHistagExpressionBacterialMutationPromoterpBADAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-Drp1
Plasmid#72927PurposeBacterial expression of mouse Drp1 isoform b (699amino acids) with C-terminal His-tag and PreScission protease cleavage siteDepositorInsertDrp1 (Dnm1l Mouse)
UseTags6xHis-tag and PreScission Protease siteExpressionBacterialMutationPromoterT7Available sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-51b-HaloTag10
Plasmid#175520PurposeExpression of HaloTag10 in bacterial cellsDepositorInsertHaloTag10
UseTagsHisExpressionBacterialMutationHaloTag10 = HaloTag7-Q165WPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET14-GFP15_Encap
Plasmid#86407PurposeExpresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operonDepositorInsertssfGFP with a 15aa CLP tag
Encapsulin
UseTags6x HistidineExpressionBacterialMutationPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJAK1.A
Plasmid#29460DepositorTypeEmpty backboneUseMycobacteria expressionTagsExpressionBacterialMutationPromoterHsp60Available sinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pT7C-HSPA1L
Plasmid#177660PurposeRecombinant protein synthesis of human HSPA1LDepositorInsertHSPA1L (HSPA1L Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIKM1
Plasmid#51054PurposeKanamycin and erethromycin resistanceDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 19, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMU-4
Plasmid#172353PurposeCRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNADepositorInsertD4-TheoR-cas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterD4Available sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAK501
Plasmid#48107PurposelacZ promoter-probe plasmid with pVS1 origin of replication and chloramphenicol resistanceDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKCiR5
Plasmid#149462PurposeBroad-Host range expression vector, RK2 and pUC origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertCinR(AM)-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPCinAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
ETS2_pet28a
Plasmid#131664PurposeBacterial expression of ETS gene ETS2DepositorInsertETS2 (ETS2 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-UBE2S
Plasmid#66714PurposeExpresses UBE2S (1-196) in E. coliDepositorInserte2-epf (UBE2S )
UseTagsGST-3CExpressionBacterialMutationPromoterT7Available sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETconNK-TEM1(S70A,D179G)
Plasmid#81166PurposepETconNK with TEM1(S70A,D179G) beta-lactamaseDepositorInsertTEM-1 beta-lactamase
UseTagsc-mycExpressionBacterial and YeastMutationDeleted the sec transport tag. Plasmid encodes H2…PromoterGAL1 and gal1Available sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
XNOR-gate-V2.0
Plasmid#44454DepositorInsertXNOR-gate
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
p7XC3H
Plasmid#47065PurposeFX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site and 10x His tagDepositorTypeEmpty backboneUseTags3C protease site (PreScission site) and decaHis (…ExpressionBacterialMutationPromoterT7Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-EPA-DNNNS-DQNRT
Plasmid#128404PurposePeriplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coliDepositorInsertExotoxin A (toxA P. aeruginosa)
UseTags6xHis tag and DsbA signal sequence for periplasmi…ExpressionBacterialMutation242DNNNS246 and 384DQNRT388 engineered glycosylat…PromotertrcAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
JMJD1CA-c089
Plasmid#79644PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertJMJD1C (JMJD1C Human)
UseTags6x His and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pTU2-a_Sv1KA_amilCP
Plasmid#204054PurposeType TU2-a backbone with kanamycin resistance, pSC101-var1 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only
