30,327 results

1. pET21b-Caspase-3(D3A,V266E)

   Plasmid

   #90091

   Purpose

   Expresses caspase D3A and V266E mutant in bacterial cells

   Depositor

   Clay Clark

   Article

   Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

   Insert

   Caspase 3 (CASP3 Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   D175A D28A D9A V266E

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cas9-PCV

   Plasmid

   #123644

   Purpose

   Expresses Cas9 with a C-terminal HUH-tag called PCV2 in E. coli

   Depositor

   Wendy Gordon

   Article

   Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

   Inserts

   PCV2 (ORF1 )
   Cas9
   

   Use

   Tags

   6xHis and SUMO

   Expression

   Bacterial

   Mutation

   2-116

   Promoter

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. 4RCI

   Plasmid

   #64653

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   YTHDF1:JMC02A-E04:C231829 (YTHDF1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   See comments

   Promoter

   T7

   Available since

   April 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pET51b(+)_LAMA-F98 (for GFP)

   Plasmid

   #130714

   Purpose

   Plasmid encoding the bacterial expression of LAMA-F98 for GFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   LAMA-F98
   

   Use

   Tags

   Hisx10

   Expression

   Bacterial

   Mutation

   Deleted first Met, Ala and Last Ser from the Enha…

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCK301

   Plasmid

   #87767

   Purpose

   E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacI

   Depositor

   John Heap

   Article

   Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

   Insert

   PrhaBAD-sfGFP
   

   Use

   Synthetic Biology

   Tags

   6xHis Tag

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaBAD rhamnose-inducible promoter from E. coli

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. GST-beta-arrestin1

   Plasmid

   #36918

   Depositor

   Robert Lefkowitz

   Article

   Nobles et al J Biol Chem. 2007 Jul 20;282(29):21370-8

   Insert

   beta-arrestin1 (Arrb1 Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 27, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pGBW-m4046979

   Plasmid

   #145611

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp7

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pBTBXh-2

   Plasmid

   #26078

   Depositor

   Ryan Gill

   Article

   Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):32

   Type

   Empty backbone

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYX-N-Puro-mNeon-WNK3

   Plasmid

   #222674

   Purpose

   endogenously tag WNK3 with N-terminal mNeon

   Depositor

   Jason Moffat

   Article

   Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

   Insert

   WNK3 (WNK3 Human)
   

   Use

   Tags

   mNeon

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. V621 pGEX-4T-LP - Acceptor

    Plasmid

    #11715

    Depositor

    Tony Pawson

    Article

    Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

    Type

    Empty backbone

    Use

    Cre/Lox; Acceptor vector

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pFG036

    Plasmid

    #137996

    Purpose

    cI repressor expression plasmid

    Depositor

    Sébastien Rodrigue

    Article

    Neil et al Commun Biol. 2020 Sep 22;3(1):523. doi:

    Insert

    cI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Thermosensitive

    Promoter

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB1H2w2-Prd

    Plasmid

    #18038

    Depositor

    Scot Wolfe

    Article

    Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

    Insert

    Paired (prd Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Paired is simply used as an insert between Kpn1 a…

    Promoter

    Available since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIgnaviCas9

    Plasmid

    #127595

    Purpose

    Expresses IgnaviCas9 in E. coli

    Depositor

    Stephen Quake

    Article

    Schmidt et al Proc Natl Acad Sci U S A. 2019 Oct 28. p

    Insert

    IgnaviCas9
    

    Use

    Tags

    6xHis Tag and Maltose binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PPIL2

    Plasmid

    #25601

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PPIL2 (PPIL2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 30, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pACYCDuet_WTDHFR_WTTS

    Plasmid

    #91226

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDF-mTET1

    Plasmid

    #81052

    Purpose

    bacterial expression of murine TET1

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TET1 (Tet1 Mouse)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAC-crRNA-Cm

    Plasmid

    #158711

    Purpose

    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    eforCP (for crRNA cloning)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEMABC

    Plasmid

    #45398

    Depositor

    Katsu Murakami

    Article

    Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

    Inserts

    rpoA (rpoA Escherichia coli)
    rpoB (rpoB Escherichia coli)
    rpoC (rpoC Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None and T7

    Available since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWT004a

    Plasmid

    #107883

    Purpose

    R1 plasmid. Contains PBAD-EGFP and is part of the CAMERA system for rewritable multi-event analog recording in bacteria

    Depositor

    David Liu

    Article

    Tang et al Science. 2018 Feb 15. pii: science.aap89

    Insert

    PBAD-EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEB2-TagRFP-T

    Plasmid

    #103998

    Purpose

    Plasmid encoding TagRFP-T

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    TagRFP-T
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (R32G, K42R, K67R, …

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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