30,152 results
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Plasmid#200424PurposethrC::(Ppen-lacIΔ11-PAmCherry1 erm2). PALM integration vector includes PAmCherry1 fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.DepositorArticleInsertLacI
UseTagsPAmCherryExpressionBacterialMutationPromoterPpenAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB007
Plasmid#98995PurposeExpress FtsI tagged with tagRFP-t in E coliDepositorInsertFtsI
UseTagsTagRFP-TExpressionBacterialMutationPromoterT5-lacAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
TTR C10S S85C
Plasmid#184410Purposebacterial expression of TTRDepositorInsertTTR (TTR Human)
UseTagsExpressionBacterialMutationC10S, S85CPromoterT7Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS hBAF53a
Plasmid#17879DepositorInsertBAF53a (ACTL6A Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBT3014
Plasmid#122540PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a late endosome localized gene (rab-7) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemOrange2::rab-7 (rab-7 Synthetic, Nematode)
UseTagsExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
saposin B^W
Plasmid#161759PurposeSaposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophanDepositorInsertsaposin B R38_W
UseTagsHis-tagExpressionBacterialMutationchanged Arginine 38 to TryptophanPromoterT7Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
COVID-SARS2 NSP16/10
Plasmid#159615PurposeBacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10DepositorInsertsCovid-SARS2 Nsp16
Covid-SARS2 Nsp10
UseTagsHis6-Zb-TEV and noneExpressionBacterialMutationCodon-optimized for E. coli expressionPromoter(Bicistronic) and T7 - LacOAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-21a_2xNLS_LbCpf1_protein_expression
Plasmid#114366PurposeProtein expression plasmid for 2xNLS LbCpf1 in pET-21a backboneDepositorInsert2xNLS_LbCpf1
UseCRISPRTagsNLSExpressionBacterialMutationPromoterT7Available sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-A4DARPin
Plasmid#198288PurposeProduces PVCs retargeted with anti-lysozyme DARPin A4DepositorInsertPVCpnf_pvc13-A4DARPin
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKD279.8
Plasmid#173171PurposeEncodes expression of SO_4388(REC)-PsdR(DBD) 137 and an sfGFP reporterDepositorInsertsSO_4388(REC)-PsdR(DBD) 137
sfGFP
UseTagsExpressionBacterialMutationPromoterJ23108 and PpsdA110Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAP2
Plasmid#171208Purposebacterial expression for purification of PGL-3, allowing cleavage for MBP tag using TEV proteaseDepositorInsertpgl-3 (pgl-3 Nematode)
UseTagsMBP::HIS::TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBZS-M.CviPII (NDel15)
Plasmid#198356PurposeExpress M.CviPII (NDel15) in bacterial cells.DepositorInsertM.CviPII
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
peSCBE3-NG-HF1-Hypa
Plasmid#218166PurposeThis plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.DepositorInsertsescbe3-NG-HF1-Hypa
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionTagsExpressionBacterialMutationMutation in the portion of deaminase hAPOBEC3A : …PromoterrpsL promoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBintT
Plasmid#48134PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2D4
Plasmid#15786DepositorInsertUbiquitin conjugating enzyme UbE2D4 (UBE2D4 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGB-GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1
Plasmid#187936PurposePlasmid for the expression and purification of GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1327DepositorUseTagsGST-mCherryExpressionBacterialMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKJ1267 (aka pAC-alphaGal4)
Plasmid#21869DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-TEV-HsRCK_296-472_E
Plasmid#146209PurposeBacterial Expression of HsRck_296-472. Contains a TEV site rather than a HRV 3C site downstream of NusA.DepositorInsertHsRck_296-472 (DDX6 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ki-67(RASA)-mCherry_IRESpuro2
Plasmid#182921PurposeExpresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.DepositorInsertMKI67 (MKI67 Human)
UseTagsEGFP and mCherryExpressionBacterial and MammalianMutationRASA mutationPromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET22-HT/spoR
Plasmid#73631PurposeProduces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tagDepositorInsertguanylate kinase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only