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Bacterial Expression

30,152 results

Showing: 7481 - 7500 of 30152 results

  3. INCENP

    Plasmid
    #39185
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    INCENP (INCENP Human)
    Use
    Tags
    GST-Tev
    Expression
    Bacterial
    Mutation
    Complex with AURKBA-c004
    Promoter
    Available since
    June 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. pET28a-B4E

    Plasmid
    #162067
    Purpose
    For expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4E
    Depositor
    Karen Polizzi
    Article
    Moya-Ramirez et al Nucleic Acids Res. 2020 Dec 16;48(22):e1
    Insert
    ampR-eIF4E fusion protein
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    eIF4E incorporating the K119A mutation, which is …
    Promoter
    T7
    Available since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    162067-plasmid-map-sequence-id-325536

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  10. pET15b LFn-Fla 3A

    Plasmid
    #84872
    Purpose
    Bacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.
    Depositor
    Russell Vance
    Article
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    Fla
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    3 C-terminal leucines have been replaced by alani…
    Promoter
    T7
    Available since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. pTSara

    Plasmid
    #60720
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    N Terminal fragment of T7 RNAP split betwen 179-180
    N Terminal fragment of T7 RNAP split betwen 179-180
    Use
    Tags
    Expression
    Bacterial
    Mutation
    T7 RNAP split between positions 179-180 and wt T7…
    Promoter
    PBAD
    Available since
    Dec. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    60720-plasmid-map-sequence-id-97706

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  14. pShoHELIX-sgRNA_entry (BO3)

    Plasmid
    #181784
    Purpose
    Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181784-plasmid-map-sequence-id-358224

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  17. pBFC0687

    Plasmid
    #186692
    Purpose
    ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_2xBsaI_NT gRNA, GmR+sfGFP cargo
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsB
    tnsC
    tniQ
    Cas12k
    Sh_2xBsaI_NT (Guide Stuffer) gRNA
    Tn7 transposon
    GmR+sfGFP ShDART Cargo
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLac
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186692-plasmid-map-sequence-id-371384

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Showing: 7481 - 7500 of 30152 results