32,662 results
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Plasmid#21869DepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 3, 2009AvailabilityAcademic Institutions and Nonprofits only
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RhoC FLARE.sc mCer, mVenus - wt
Plasmid#65071PurposeRhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoCDepositorInsertRBD, mCerulean, mVenus, RhoC
ExpressionBacterial, Insect, and Mamm…MutationT153M in mVenusPromoterCMV, p10Available SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJMP2832
Plasmid#160672PurposeMobile CRISPRi sfGFP 'test', gmc6 sgRNADepositorInsertgmc6 (gfp) sgRNA
ExpressionBacterialAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2820
Plasmid#160669PurposeMobile CRISPRi sfGFP 'test', empty sgRNADepositorInsertempty sgRNA (BsaI)
ExpressionBacterialAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCmEos3.2:cat
Plasmid#78100PurposePlasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.DepositorInsertmEos3.2-cat
ExpressionBacterialAvailable SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKERepPar
Plasmid#211827PurposeMedium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.DepositorInserteGFP
ExpressionBacterialPromoterT5Available SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGal CycK (P#844)
Plasmid#14664DepositorInsertCycK (CCNK Human)
ExpressionBacterialAvailable SinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT23
Plasmid#89663Purposeexpression of doubleTAL staple protein dT23 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT23
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG4
Plasmid#70115PurposeTn7-based lux reporter for promoter cloning in bacteriaDepositorTypeEmpty backboneExpressionBacterialPromoternoneAvailable SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1_GST-PLproCS-MBP
Plasmid#169195PurposeTo express a PLpro substrate (GST-PLproCS-MBP) in E. coliDepositorInsertGST-PLproCS-MBP
TagsGST-MBP fusion (PLpro-cleavable) and His6 (intern…ExpressionBacterialPromotertac promoterAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1
Plasmid#164614PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.DepositorInsertstdMCP-stdHalo-RH1
UseLentiviralTagsHA tag and NLSExpressionBacterial and MammalianPromoterUbiCAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI
Plasmid#115207PurposeExpresses BzoCas13b from a SUMO-tagged bacterial expression constructDepositorInsertBzoCas13
TagsSUMOExpressionBacterialAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA-32Ala9
Plasmid#102616PurposeExpression of human RPA with an alanine substituted phosphorylation domain (with the following mutations in RPA32 :S8,11-13,23,29,33,39A,T21A).DepositorExpressionBacterialMutationwith an alanine substituted phosphorylation domainPromoterT7Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRedCm
Plasmid#176225PurposeLambdaRed-expressing plasmid carrying the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phageDepositorInsertLambda Red operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
UseSynthetic BiologyExpressionBacterialPromoterPrhaB and PLAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_ionRD
Plasmid#172924PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.DepositorInsertIonicStrengthSensorRD
TagsHisExpressionBacterialPromoterT7Available SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pADS
Plasmid#19040DepositorInsertcodon-optimized synthetic amorphadiene synthase
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBADHISTEV_PfeE
Plasmid#129120PurposepBADHISTEV carrying pfeE for overexpression of PfeE in E. coliDepositorInsertPeriplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
TagsTEV protease cleavable 6xHisExpressionBacterialMutationSignal peptide sequence has been removedPromoterPBADAvailable SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPro33
Plasmid#17810DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceApril 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBZS-YP_001497877
Plasmid#198366PurposeExpress YP_001497877 in bacterial cells.DepositorInsertYP_001497877 (B681L )
ExpressionBacterialAvailable SinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-H698N
Plasmid#92242PurposeExpress H698N mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-H698N mutant
Tags6x-Histag, TEV cleavage siteExpressionBacterialMutationHistidine 698 to AsparaginePromoterT7 promoterAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only