30,811 results

1. 5H9M (SIAH2)

   Plasmid

   #79521

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SIAH2 (SIAH2 Human)
   

   Tags

   8xHis-Thrombin-SUMO-TEV

   Expression

   Bacterial

   Promoter

   T7-lacO

   Available Since

   June 15, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pBAD33.1-DpnII

   Plasmid

   #127413

   Purpose

   Expresses DpnII restriction endonuclease in bacteria

   Depositor

   Douglas Feldman

   Insert

   DpnII
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAP24

   Plasmid

   #70190

   Purpose

   Modular vector for secreted luciferase reporter expression

   Depositor

   Wiep Klaas Smits

   Article

   Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

   Insert

   sLucopt
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Ptet

   Available Since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDipA-TEV-His12

   Plasmid

   #137042

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
   

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length protein, contains signal p…

   Promoter

   T7

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-Neq2X

   Plasmid

   #182365

   Purpose

   Expresses Neq2X DNA polymerase

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

   Insert

   Neq2X
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSKB-

   Plasmid

   #62540

   Purpose

   Cloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate Cloning

   Depositor

   Eric Marois

   Article

   Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET26b_Nb.AT110

   Plasmid

   #135480

   Purpose

   E. coli periplasmic expression of conformational nanobody Nb.AT110 recognizing angiotensin II type 1 receptor

   Depositor

   Andrew Kruse

   Article

   Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

   Insert

   Nanobody AT110
   

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX6P1-hPREP

   Plasmid

   #179387

   Purpose

   Express human prolyl oligopeptidase (tagged) in bacterial cells

   Depositor

   Christian Gruber

   Article

   Retzl et al J Nat Prod. 2020 Nov 25;83(11):3305-3314

   Insert

   human prolyl oligopeptidase (PREP Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   TAC

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBTB-3

   Plasmid

   #22819

   Depositor

   Ryan Gill

   Article

   Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   March 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJOE7771.1

    Plasmid

    #135077

    Purpose

    Mannitol, arabitol, and glucitol-inducible mtlR/PmtlE expression vector

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al PLoS One. 2015 Jul 24;10(7):e0133248. do

    Insert

    mtlR
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. B1-4

    Plasmid

    #111466

    Purpose

    translational elongation factor Tu

    Depositor

    Cheemeng Tan

    Article

    Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

    Insert

    translational elongation factor Tu
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET47b-T7-AsCas12f-WT

    Plasmid

    #204640

    Purpose

    Expression of wild-type AsCas12f in E. coli

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    AsCas12f
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAD/HisD-LSSmKate2

    Plasmid

    #31909

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    LSSmKate2
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKJ1267 (aka pAC-alphaGal4)

    Plasmid

    #21869

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Protoc. 2009. 4(10):1471-501.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKB223

    Plasmid

    #170013

    Purpose

    Encodes LacI-controlled expression of surface-displayed C18G and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    C18G
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Promoter

    Ptac and PvirK

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. KLHL3 (KLHL3A-c003)

    Plasmid

    #110251

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KLHL3 (KLHL3 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7 - lacO

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ec2Avs2

    Plasmid

    #188790

    Purpose

    Avs2 from Escherichia coli NCTC10650

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

    Insert

    Ec2Avs2
    

    Expression

    Bacterial

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNAD1

    Plasmid

    #154347

    Purpose

    pUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistance

    Depositor

    John van der Oost

    Article

    Naduthodi et al Biotechnol Biofuels. 2019 Mar 25;12:66.

    Insert

    ShBle
    

    Use

    Microalgae

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Violaxanthin Chlorophyll a Binding Protein Precur…

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221-SAR1-no stop

    Plasmid

    #67345

    Purpose

    hSar1 without stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SAR1 (SAR1A Human)
    

    Use

    Gateway entry vector

    Expression

    Bacterial

    Promoter

    None

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pJDC182

    Plasmid

    #60854

    Purpose

    Expresses firefly luciferase

    Depositor

    Jeffrey Cirillo

    Article

    Chang et al PLoS One. 2014 Sep 29;9(9):e108341. doi:

    Insert

    Firefly luciferase (optimized for Mycobacteria)
    

    Expression

    Bacterial

    Mutation

    codon optimized for Mycobacteria

    Promoter

    hsp60

    Available Since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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