30,176 results

1. pET Biotin His6 mCitrine LIC cloning vector (H6-mCitrine)

   Plasmid

   #29724

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, AviTag (biotin), TEV cleavage site, and mC…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKIKan_idCas9_Chr_center

   Plasmid

   #149637

   Purpose

   suicide vector for knock in into the B. burgdorferi chromosome

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pnxABE-flgn

   Plasmid

   #187932

   Purpose

   A-to-G base editor

   Depositor

   Yi Xiao

   Article

   et al ACS Synth Biol. 2022 Apr 15;11(4):1650-1

   Insert

   ecTadA(8e)-nxCas9 (D10A)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTWIN1-His6-Ssp-Httex1-23Q

   Plasmid

   #84349

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon 1 (HTT Human)
   

   Use

   Tags

   N-terminal Ssp intein (His-tagged)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCM291rfp

   Plasmid

   #59775

   Purpose

   pCMrfp with TrfA mutation (291E to G)

   Depositor

   Nathan Hillson

   Article

   Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

   Insert

   rfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET27b-ecDHFR-N23PP/L28F

   Plasmid

   #118584

   Purpose

   Bacterial expression of E.coli DHFR N23PP and L28F mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

   Insert

   DHFR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   L28 mutated to F28, N23 mutated to P23, a proline…

   Promoter

   T7

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET22b-ecDHFR

   Plasmid

   #109055

   Purpose

   Bacterial expression of E.coli DHFR protein

   Depositor

   Stephen Benkovic

   Article

   Cameron et al Biochemistry. 1997 Dec 16;36(50):15792-8

   Insert

   ecDHFR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. L1-1180

   Plasmid

   #13265

   Depositor

   Vance Lemmon

   Article

   Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

   Insert

   L1CAM (L1CAM Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Truncated after RSLE region

   Promoter

   Available since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pBAD/HisD-LSSmKate1

   Plasmid

   #31908

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   LSSmKate1
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

   Promoter

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBXNPH3

    Plasmid

    #110098

    Purpose

    E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag and 3C cleavage site

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Type

    Empty backbone

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUC19-T7-SdB-T

    Plasmid

    #87307

    Purpose

    Expresses the fluorescence-activating Stabilized dimeric Broccoli (SdB) aptamer under the control of the T7 promoter.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Stabilized dimeric Broccoli aptamer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET24b-BR1.0

    Plasmid

    #199435

    Purpose

    expresses BAmRed1.0 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMRed
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pmCellFree_KA1 ZIKV E

    Plasmid

    #145341

    Purpose

    Cell-free/mammalian expression vector for Zika virus Envelope protein with N-term eGFP

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV Envelope
    

    Use

    Tags

    8xHis and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7- CMV/CAG

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. POLN_pDEST17

    Plasmid

    #64885

    Purpose

    bacterial expression of POLN for purification

    Depositor

    Richard Wood

    Article

    Takata et al Nucleic Acids Res. 2010 Jun;38(10):3233-

    Insert

    POLN (POLN Human)
    

    Use

    Tags

    Flag and His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBS mouse IRS-2

    Plasmid

    #11372

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-2 (Irs2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 1, 2006

    Availability

    Academic Institutions and Nonprofits only

16. KIF3A560GFP

    Plasmid

    #129769

    Purpose

    Expresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged

    Depositor

    William Hancock

    Insert

    KIF3A (Kif3a Mouse)
    

    Use

    Tags

    GFP and His6

    Expression

    Bacterial

    Mutation

    Truncation at 359, fused to kinesin-1 starting Al…

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. human p53-(1-393)-L344P

    Plasmid

    #24862

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Leucine 344 mutated to Proline

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pET21c-eIF4A

    Plasmid

    #37230

    Depositor

    Jon Lorsch

    Article

    Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

    Insert

    TIF1 (TIF1 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

19. SUMO_TwinStrep_Csx30

    Plasmid

    #197611

    Purpose

    For protein purification of Csx30

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2022 Nov 25;378(6622):874-881.

    Insert

    SUMO_TwinStrep_Csx30
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEE050

    Plasmid

    #176807

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    601
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge