32,838 results
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Plasmid#235474PurposeInducible gene VIII by Ptet promoterDepositorInsertphage M13 geneVIII
ExpressionBacterialAvailable SinceMay 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG054
Plasmid#231323PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG010, pOGG012, and pOGG014, NmR/KmRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMW364
Plasmid#238205PurposeExpresses untagged TasR from a PBAD promoter, followed by 3xRNA1 TIGR arrayDepositorInsertTaTasR
ExpressionBacterialAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
BLA_CAT_K136_GPG_LOV2
Plasmid#231575PurposeThis plasmid mediates chloramphenicol resistance in the dark, resulting in Cm mediated cell death upon illumination with blue lightDepositorInsertCAT_ carrying an AsLOV2 insertion behind K136
ExpressionBacterialAvailable SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SET
Plasmid#234708Purposeexpresses His-tagged human SET in E coliDepositorAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTn7xTS_SnoopCatcher:mNG
Plasmid#225191PurposeUsed for inserting SnoopCatcher fused to mNeonGreen at the bacterial Tn7 siteDepositorInsertSnoopCatcher:mNeonGreen
TagsSnoopCatcherExpressionBacterialAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGY18
Plasmid#221708PurposeEmpty Cas9 vector for multiplex gene editing in fungiDepositorTypeEmpty backboneUseCRISPR; AspergillusExpressionBacterialAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJAK184.tetR.PT5-3.dCas9
Plasmid#232482Purposeoptimized ahTet inducible dCas9 for insertion into C. difficile genomeDepositorInsertCatalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
UseCRISPRExpressionBacterialPromoterTetR-PT5-3Available SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJC.15A.sgRNA.TA
Plasmid#232483PurposesgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.DepositorInsertsToxin-antitoxin System from CD630
medium-copy-number p15A origin of replication
mCherry
UseCRISPRExpressionBacterialPromoterP3 constitutive promoter and native promoterAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAED2
Plasmid#234599PurposeFluorescent reporter of the SOS responseDepositorInsertsmScarlet-I
gfp-mut2
UseReporterExpressionBacterialMutationGFPmut2 was derived from avGFP with the following…PromoterPtet+dnaK P1 and cdaAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-DiCas7-11-His
Plasmid#234482PurposePlasmid for bacterial expression and purification of DiCas7-11 protein (human codon-optimized)DepositorInsertDiCas7-11
UseCRISPRTags6xHisExpressionBacterialAvailable SinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG GST Empty
Plasmid#236735PurposeEmpty bacterial expression plasmid for GST fusion proteinsDepositorTypeEmpty backboneTagsGSTExpressionBacterialAvailable SinceApril 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAG GST EGFP
Plasmid#236736PurposeBacterial expression plasmid containing GST-tagged EGFPDepositorInsertEGFP
TagsGSTExpressionBacterialPromoterTacAvailable SinceApril 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCascade_SPS1(PmcCAST)
Plasmid#224926PurposeExpression and purification of type I-B2 Cascade in CRISPR-associated transposon of RomsettaDepositorInsertsCas5, Cas6, Cas7, Cas8, Cas11
CRISPR assay for crRNA expression
UseCRISPRExpressionBacterialAvailable SinceApril 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFNC-5
Plasmid#233297PurposePlasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. TcR, easily curable via sucrose counterselection.DepositorInsertsBxbI phage integrase
sacB
ExpressionBacterialPromoterPEM7 and unknownAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3238
Plasmid#234092PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertsfCherry2
ExpressionBacterialMutationE117Q/T127I/G219APromoterpJ23100-BsaIAvailable SinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
p370_DG
Plasmid#234693PurposeGolden gate domesticated pET28b with 3C-mNeongreen-His10DepositorTypeEmpty backboneTags3C-mNeongreen-His10ExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p368_DG
Plasmid#234691PurposeGolden gate domesticated pET28b with 3C-His6DepositorTypeEmpty backboneTags3C-His6ExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p369_DG
Plasmid#234692PurposeGolden gate domesticated pET28b with 3C-His10DepositorTypeEmpty backboneTags3C-His10ExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p361_DG
Plasmid#234683PurposeGolden gate domesticated pET28b with His6-3C-3XFLAG-SUMO and Thrombin-HiBiTDepositorTypeEmpty backboneTagsHis6-3C-3XFLAG-SUMO and Thrombin-HiBiTExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p354_DG
Plasmid#234697PurposeGolden gate domesticated pET28b for scarless cloning with His10-TEVDepositorTypeEmpty backboneTagsHis10-TEVExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p359_DG
Plasmid#234702PurposeGolden gate domesticated pET28b for scarless cloning with His10-mNeongreen-3CDepositorTypeEmpty backboneTagsHis10-mNeongreen-3CExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p346_DG
Plasmid#234676PurposeGolden gate domesticated pET28b with His10-TEVDepositorTypeEmpty backboneTagsHis10-TEVExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p347_DG
Plasmid#234677PurposeGolden gate domesticated pET28b with His10-ThrombinDepositorTypeEmpty backboneTagsHis10-ThrombinExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL31
Plasmid#227428PurposeKanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)DepositorInsertmWasabi
UseCRISPRExpressionBacterialAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL32
Plasmid#227429PurposeKanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)DepositorInserttdTomato
UseCRISPRExpressionBacterialAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRecE564T
Plasmid#226825PurposeExpressing RecE*T controled by Ptrc promoterDepositorInsertrecE*T
ExpressionBacterialAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pISFba1
Plasmid#226820PurposeExpressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISFba1 guide RNA targeting pMB1 oriDepositorInsertISFba1
ExpressionBacterialAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2641
Plasmid#229525PurposeCarries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
UseCRISPRTagst2a-mNeonGreen-AID*ExpressionBacterial and YeastMutationF74GPromoterTEF1Available SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2644
Plasmid#229528PurposeCarries AID* tag, empty sgRNA, and NATR markerDepositorInsertAID* - empty sgRNA - NATR
Tags2xFLAGExpressionBacterial and YeastAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only