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pTn7-SCOUT11.G

Plasmid

#200999

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-sfGFP, GmR, ApR

Depositor

Philip Poole

Publication

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

superfolder GFP

Expression

Bacterial

Promoter

J23104

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-Ptet2

Plasmid

#218368

Purpose

Empty backbone for Tn7-site insertion with tetR-PtetA expression system with two tetR operator sites

Depositor

Michael Gebhardt

Publication

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCL1333

Plasmid

#217966

Purpose

Bacterial expression of RAN with an N-terminal 6His tag and a TEV cleavage site.

Depositor

Andrew Bowman

Publication

Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

Insert

RAN (RAN Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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Luciferase

Plasmid

#213979

Purpose

Expresses Doxy Inducible Luciferase

Depositor

Coleman Lindsley

Publication

Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

Insert

Luciferase

Use

Lentiviral

Tags

6xHis affinity tag

Expression

Bacterial and Mammalian

Promoter

Tet-responsive promoter PTight, consisting of sev…

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NylCA-TS_pET28a

Plasmid

#215419

Purpose

Expression construct for NylCA-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Publication

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCA-TS

Expression

Bacterial

Mutation

S111G/A137L/E263Q

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FsC_pET29b

Plasmid

#215405

Purpose

Expression construct for FsC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Publication

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

FsC

Expression

Bacterial

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPbU6_hdhfr/yfcu_Cas9

Plasmid

#216423

Purpose

Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.

Depositor

Ellen Bushell

Publication

Jonsdottir et al bioRxiv 2024.04.20.590404

Type

Empty backbone

Use

CRISPR; Expression in plasmodium berghei

Expression

Bacterial

Available since

April 28, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Spacer Switch Plasmid

Plasmid

#215346

Purpose

Facilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.

Depositor

Hank Seifert

Publication

Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

Insert

OpaD spacer; Chloramphenicol Resistance Cassette

Use

CRISPR

Expression

Bacterial

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-C5E-6XTUBE

Plasmid

#217628

Purpose

Bacterial expression of MBP tagged 6XTUBE

Depositor

Ethan Lee

Publication

Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

Insert

6XTUBE

Expression

Bacterial

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBroad-T7RNAP-apraR

Plasmid

#199993

Purpose

T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) apraA

Depositor

Farren Isaacs

Publication

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

T7RNAP Circuit

Expression

Bacterial

Mutation

copFnull

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pACRISPR-TP

Plasmid

#203589

Purpose

A sgRNA expression plasmid for genome editing in Burkholderia

Depositor

Leonilde Moreira

Publication

Ferreira et al Appl Environ Microbiol. 2024 Feb 21;90(2

Type

Empty backbone

Expression

Bacterial

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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OPEN Beta2-microglobulin

Plasmid

#215657

Purpose

E. coli expression of human beta-2-microglobulin containing a cysteine mutation for disulfide linkage to mutant HLA.

Depositor

Nikolaos Sgourakis

Publication

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN Beta2-microglobulin (B2M Human)

Expression

Bacterial

Mutation

Histidine 31 to Cysteine

Promoter

T7

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_his

Plasmid

#210405

Purpose

For expression of VHH with C-terminal his tag

Depositor

Ray Owens

Publication

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Tags

his6

Expression

Bacterial

Promoter

lac

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pColdI-GST-mGTSF1

Plasmid

#214671

Purpose

Bacterial expression of GST-tagged mouse GTSF1

Depositor

Phillip Zamore

Publication

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

GTSF1 (Gtsf1 Mouse)

Tags

6x His and GST

Expression

Bacterial

Promoter

CspA

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pFN18A-HaloTag-Biotin

Plasmid

#206039

Purpose

HaloTag-(Ig32)2-Talin R3-IVVI-(Ig32)2-10xHisTag-AviTag

Depositor

Sergi Garcia-Manyes

Publication

Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

Insert

Talin R3-IVVI (Tln1 Mouse)

Tags

10 x His-AviTag and Halo-Tag

Expression

Bacterial

Mutation

Threonine 809 to Isoleucine, Threonine 833 to Val…

Promoter

T7

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCT02

Plasmid

#212152

Purpose

Plasmid expressing 6x His tagged FAST-PETase under inducible promoter PT7 control.

Depositor

Kang Zhou

Insert

FAST-PETase

Tags

6x His

Expression

Bacterial

Mutation

S121E, D186H, R224Q, N233K, R280A

Promoter

PT7

Available since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGR618

Plasmid

#213786

Purpose

Integrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette

Depositor

Chang Liu

Insert

BB-Tv TP-DNAP1

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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SPLICSFAST-NU-MT-P2A

Plasmid

#214898

Purpose

Expresses equimolar Nucleus- and Mitochondria- targeted split-FAST fragments for Short-range NU-MT Contact sites

Depositor

Tito Calì

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SPLICSFAST-NU-MT-P2A

Tags

none

Expression

Bacterial and Mammalian

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-1α

Plasmid

#214341

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Publication

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL1A (IL1A Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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SurA

Plasmid

#213138

Purpose

Expresses chaperone SurA

Depositor

Sebastian Hiller

Publication

Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

Insert

SurA (21-428) (surA E.coli)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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