30,176 results

1. PET-3a-chpBK

   Plasmid

   #155201

   Purpose

   To express recombinant ChpBK protein

   Depositor

   Guanzheng Luo

   Article

   Zhang et al Sci Adv. 2019 Jul 3;5(7):eaax0250. doi:

   Inserts

   chpS-chpBK-6×His-Tag
   GyrA-CBD-10xHis-Tag
   

   Use

   Tags

   10xHis and 6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDSG321

   Plasmid

   #115598

   Purpose

   surface-displayed Nb3, used in composability experiments; medium copy

   Depositor

   Ingmar Riedel-Kruse

   Article

   Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

   Insert

   regulated adhesin construct
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRE-Tn5-150

   Plasmid

   #118532

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   TagBFP2
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEO100c

   Plasmid

   #50550

   Purpose

   Expresses sfGFP under the PompC1157 promoter

   Depositor

   Jeffrey Tabor

   Article

   Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 22, 2014

   Availability

   Academic Institutions and Nonprofits only

5. RBM4_2xRRM-ZNF_pGEX

   Plasmid

   #135154

   Purpose

   Recombinant expression of RBM4 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   RBM4 (RBM4 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-GGG-HIS-CBM-Xmod-DocIII

   Plasmid

   #91698

   Purpose

   CBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGG

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   GGG-HIS-CBM-Xmod-DocIII
   

   Use

   Tags

   HIS-Tag and Sortase-Tag

   Expression

   Bacterial

   Mutation

   Cysteine deletion in CBM C63S

   Promoter

   T7

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPEP1

   Plasmid

   #61046

   Purpose

   BglFusion compatible integration vector for Streptococcus pneumoniae

   Depositor

   Jan-Willem Veening

   Article

   Sorg et al ACS Synth Biol. 2014 Jun 4.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRSFDuet-1_VNp-LZ_VNp-LZ-hGH

   Plasmid

   #182412

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine zipper-human growth hormone fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Inserts

   VNp-LZ-hGH (GH1 Human)
   VNp-LZ
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-R-GECO1.2

   Plasmid

   #113937

   Purpose

   Bacterial expression of red fluorescent Ca2+ sensor R-GECO1.2

   Depositor

   Robert Campbell, Thomas Hughes

   Article

   Molina et al Biophys J. 2019 May 21;116(10):1873-1886

   Insert

   R-GECO1.2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a- mYFP-kanMX6

    Plasmid

    #105147

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu, Thomas Pollard

    Article

    Wu et al Dev Cell. 2003 Nov;5(5):723-34.

    Insert

    mYFP-kanMX6
    

    Use

    Tags

    mYFP

    Expression

    Bacterial and Yeast

    Mutation

    A206K

    Promoter

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. H11-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

    Plasmid

    #180156

    Purpose

    tdTomato cre reporter with Hipp11 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    tdTomato
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAC-crRNA-Km

    Plasmid

    #158712

    Purpose

    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    AcGFP (for crRNA cloning)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-KG-CAND1

    Plasmid

    #19949

    Depositor

    Yue Xiong

    Article

    Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

    Insert

    CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pBbE8c-RFP

    Plasmid

    #35269

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BAD

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHS1184

    Plasmid

    #205965

    Purpose

    NZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    DinG HNH
    

    Use

    Tags

    14xHis-TwinStrep-bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJUMP27-1A(sfGFP)

    Plasmid

    #126974

    Purpose

    Level 1 vector. sfGFP reporter. Origin pSC101 (low copy number)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-29b(+)_NSP4ss_SerM_NucA-myc

    Plasmid

    #218191

    Purpose

    Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)

    Depositor

    Florence Pojer

    Insert

    nucA
    

    Use

    Tags

    NSP4 signal secretion peptide and myc

    Expression

    Bacterial

    Mutation

    codon optimized ecoli residues - 22-266

    Promoter

    T7

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKS2

    Plasmid

    #24625

    Depositor

    Jay Keasling

    Article

    Steen et al Nature. 2010 Jan 28;463(7280):559-62.

    Insert

    'tesA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGagMBPHis

    Plasmid

    #42176

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    Gag protein sequence is from HIV-1 clone NL4-3
    

    Use

    Tags

    6xHIS and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pET24c(+)-mtEFTu

    Plasmid

    #30535

    Depositor

    Linda Spremulli

    Article

    Woriax et al Biochim Biophys Acta. 1995 Dec 27. 1264(

    Insert

    Mature bovine mitochondrial elongation factor Tu
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Mature form amino acids 44-452. Mito import seque…

    Promoter

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only