31,176 results
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Plasmid#81163PurposepSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1DepositorInsertTEM-1 beta-lactamase
TagsCodon swapped TEM-1 from H26 to W290 and His6xExpressionBacterialMutationDeleted the sec transport tag. Plasmid encodes H2…Promoterlac promoterAvailable SinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEYN12-mflon
Plasmid#165581PurposeLon protease from Mesoplasma florumDepositorInsertlon protease
ExpressionBacterialPromoterPtrcAvailable SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
NylB'_pET21b
Plasmid#215416PurposeExpression construct for NylB' gene, codon optimized for expression in E. coliDepositorInsertNylB'
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP3_Sap
Plasmid#206849PurposeA plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was domesticated for SapI and integrated into the chloroplast fragment.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP2
Plasmid#206847PurposeA plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP1
Plasmid#206846PurposeA plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPANI2A2E2-Crimson
Plasmid#193758PurposeExpresses E2-Crimson under the control of PANI2 synthetic promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertE2-Crimson
UseSynthetic BiologyExpressionBacterialPromoterPANI2 synthetic promoter carrying binding sites f…Available SinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_tacI
Plasmid#155170PurposeBaSP and CuCBP co-expression: tacI promoter controls BaSP expressionDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET sfGFP(150TAG)
Plasmid#133455Purposeexpresses sfGFP with an unnatural amino acid at the 150th position in bacterial cellsDepositorInsertsfGFP
TagsHisExpressionBacterialPromoterT7Available SinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdTomato
Plasmid#163443PurposeBacterial expression of His-tagged, SV40 NLS-tagged tdTomato in the modified pRSETB protein expression backboneDepositorInserttdTomato
Tags6xHisExpressionBacterialPromoterT7Available SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBR5pemIKpBAD
Plasmid#129273PurposeBroad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and arabinose-inducible promoterDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-67/68
Plasmid#113922PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68DepositorInsertMBP-NanoLucCP-67/68
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCHERRY10
Plasmid#24664DepositorInsertmCherry
ExpressionBacterialAvailable SinceJuly 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
PE-SUMO CRYAB
Plasmid#217670PurposeFor expression of SUMO tagged human alpha B crystallinDepositorAvailable SinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP561
Plasmid#222723PurposeTarget and Recombinase Golden Gate Landing Pad Backbone for LT-RT with T7 RecombinaseDepositorTypeEmpty backboneTags6xHisExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
ALC1-c005
Plasmid#173092PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRU1144
Plasmid#14471DepositorInsertmRFP1
ExpressionBacterialAvailable SinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-AcKRS-CloDF
Plasmid#127415PurposeMutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophageDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
ExpressionBacterialPromoteraraBADAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-AcrVA5-lenti
Plasmid#115664PurposeExpresses AcrVA5 in human cells, contained in a lentiviral vector.DepositorInsertAcrVA5
UseLentiviralExpressionBacterial and MammalianPromoterEF-1a promoterAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1 METTL3
Plasmid#200731PurposeBacterial expression of GST-Tagged codon optimized human METTL3DepositorInsertMETTL3 (METTL3 Human)
TagsGST from backbone plasmidExpressionBacterialMutationCodon optimized for expression in E. coliAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only