30,844 results

1. pCB

   Plasmid

   #162699

   Purpose

   Expresses pHnCB10 derived carboxysome operon and prk

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   pHnCB10 derived carboxysome operon, prk
   

   Expression

   Bacterial

   Promoter

   PLtet0-1 promoter

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWaldo-GFPd_PepTSt

   Plasmid

   #58333

   Purpose

   Expresses the POT family transporter from Streptococcus thermophilus in E. coli as a C-terminally tagged GFP his tagged protein

   Depositor

   Simon Newstead

   Article

   Solcan et al EMBO J. 2012 Aug 15;31(16):3411-21. doi:

   Insert

   PepTSt
   

   Tags

   GFP-His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pET His6 msfGFP TEV cloning vector with BioBrick polycistronic restriction sites (9GFP)

   Plasmid

   #48287

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   His6 and msfGFP

   Expression

   Bacterial

   Available Since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. NUDT1

   Plasmid

   #74660

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   NUDT1 (NUDT1 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pJV0042

   Plasmid

   #205138

   Purpose

   Expression of 6xHis-EGFP-SnxA in bacteria

   Depositor

   Jason King

   Article

   Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

   Insert

   SnxA
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)

   Plasmid

   #166421

   Purpose

   Express pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Naegleria fowleri PRL
   

   Tags

   N-terminal His-tag

   Expression

   Bacterial

   Mutation

   Deleted nucleotides coding for 4 C-terminal amino…

   Promoter

   AmpR, lac1

   Available Since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. 6xHis(TEV)-Drp1(K38A)-StrepII

   Plasmid

   #174423

   Purpose

   Expresses human Drp1 Isoform 3 with K38A mutation in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Tags

   6xHis(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   K38A

   Promoter

   T7

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pScaf-5544.4

   Plasmid

   #111524

   Purpose

   pScaf phagemid with insert for generating DNA origami scaffold 5544 bases in length (v4)

   Depositor

   Shawn Douglas

   Article

   Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

   Insert

   pScaf-KH'
   

   Expression

   Bacterial

   Available Since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-Drp1

   Plasmid

   #72927

   Purpose

   Bacterial expression of mouse Drp1 isoform b (699amino acids) with C-terminal His-tag and PreScission protease cleavage site

   Depositor

   David Chan

   Article

   Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

   Insert

   Drp1 (Dnm1l Mouse)
   

   Tags

   6xHis-tag and PreScission Protease site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBT3000

    Plasmid

    #122536

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (tram-1) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_CemOrange2::tram-1 (tram-1 Synthetic, Nematode)
    

    Expression

    Bacterial

    Promoter

    Pnhx-2

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. GAL4-BRD4

    Plasmid

    #21943

    Depositor

    Eric Verdin

    Article

    Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

    Insert

    BRD4 (BRD4 Human)
    

    Tags

    Flag and GAL4-DNA-BD

    Expression

    Bacterial

    Mutation

    aa 1209-1362

    Available Since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

12. EcMBP165-cpGFP.PPYF.pRSET

    Plasmid

    #33372

    Purpose

    a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

    Depositor

    Loren Looger

    Article

    Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

    Insert

    MBP based Maltose Biosensor
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    cpGFP146 variant from GCaMP2 inserted between AA1…

    Promoter

    T7

    Available Since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCOLADuet-1_nsp10 (SARS-CoV-2)

    Plasmid

    #169158

    Purpose

    To express SARS-CoV-2 nsp10 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp10 (ORF1ab SARS-CoV-2)
    

    Tags

    Methionine

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. JF001A

    Plasmid

    #53116

    Purpose

    expresses alpha hemolysin under the control of theophylline riboswitch

    Depositor

    Sheref Mansy

    Article

    Lentini et al Nat Commun. 2014 May 30;5:4012. doi: 10.

    Insert

    theophylline riboswitch and alpha hemolysin
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    May 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

    Plasmid

    #122085

    Purpose

    Express M.EcoGII fused to CENPC with destabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII-V5-CENPC (CENPC E.coli, Human)
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of CENPC

    Expression

    Bacterial and Mammalian

    Mutation

    L341F, R930L (see comments below)

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. E. coli expression: Z-lock cofilin dark state mutant

    Plasmid

    #141135

    Purpose

    a control for optogenetic cofilin analog

    Depositor

    Klaus Hahn

    Article

    Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

    Insert

    Zdk2, Cofilin, LOV2
    

    Expression

    Bacterial

    Mutation

    D3 deletion, I32F in Zdk2

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. GST-cdc42

    Plasmid

    #12175

    Depositor

    Marc Kirschner

    Insert

    cdc42 (CDC42 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 7, 2006

    Availability

    Academic Institutions and Nonprofits only

18. p15A-OptoCre-tetA-Ptet-Rtet

    Plasmid

    #189855

    Purpose

    Cre-inducible activation of tetA for tetracycline resistance.

    Depositor

    Mary Dunlop

    Article

    Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

    Insert

    Ptet-loxP-TT-loxP-Rtet-tetA
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Ptet

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJB153

    Plasmid

    #92328

    Purpose

    Express mEos3.2 tagged ZapA in E coli

    Depositor

    Jie Xiao

    Article

    Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

    Insert

    ZapA
    

    Tags

    mEos3.2

    Expression

    Bacterial

    Promoter

    T5-lac

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. VPS4B, S2C

    Plasmid

    #184806

    Purpose

    Bacterial expression of VPS4B (S2C mutation)

    Depositor

    James Hurley

    Article

    Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

    Insert

    VPS4B (VPS4B Human)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    S2C

    Promoter

    T7

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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