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32,218 results

Showing: 11461 - 11490 of 32218 results

  1. pDule-IBBN (G2)

    Plasmid
    #85500
    Purpose
    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-
    Insert
    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32G L65E F108W Q109M D158S R162K
    Promoter
    lpp (constitutive)
    Available Since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    85500-plasmid-map-sequence-id-169863

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  5. p405CYC1

    Plasmid
    #15967
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    p405CYC1 (CYC1 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    CYC1 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15967-plasmid-map-sequence-id-8452

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  7. pSP019

    Plasmid
    #168195
    Purpose
    expresses proteolytically inactive EatA passenger domain with H134R mutation within the protease catalytic triad.
    Depositor
    James Fleckenstein
    Insert
    eatA[H134R]
    Expression
    Bacterial
    Mutation
    H134R mutation
    Promoter
    araBAD
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    168195-plasmid-map-sequence-id-339468

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  8. pZH517

    Plasmid
    #102670
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102670-plasmid-map-sequence-id-199582

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  20. pHN873

    Plasmid
    #178829
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A (AR Human)
    Tags
    MBP, His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178829-plasmid-map-sequence-id-351932

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  22. pET15-EF1p_mFAP2b

    Plasmid
    #159469
    Purpose
    Encodes for EF1p_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF1p_mFAP2b
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Available Since
    Dec. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    159469-plasmid-map-sequence-id-319725

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Showing: 11461 - 11490 of 32218 results