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k3(long) luciferase

Plasmid

#11110

Depositor

Anjana Rao

Article

Tsai et al Mol Cell Biol. 1996 Feb . 16(2):459-67.

Insert

k3(L) (TNF Human)

Tags

luciferase

Expression

Bacterial

Available Since

April 24, 2006

Availability

Academic Institutions and Nonprofits only
ETV2_pet28a

Plasmid

#131665

Purpose

Bacterial expression of ETS gene ETV2

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV2 (ETV2 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pKB817 (pBra-Hfq)

Plasmid

#174661

Purpose

Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Hfq. Useful as three-hybrid positive control.

Depositor

Katherine Berry, Ann Hochschild

Article

Berry et al Nucleic Acids Res. 2018 Jan 25;46(2):e12

Insert

Hfq

Tags

alpha NTD

Expression

Bacterial

Promoter

lpp/lacUV5 (tandem promoter)

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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mRuby-pBAD

Plasmid

#54763

Purpose

Localization: Protein Expression Vector , Excitation: 558, Emission: 605

Depositor

Michael Davidson

Type

Empty backbone

Tags

6xHis and mRuby

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pPEPY-Plac

Plasmid

#122634

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae

Depositor

Jan-Willem Veening

Article

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST17-cDAXX

Plasmid

#169729

Purpose

Bacterial expression of N-terminally 6His tagged cDAXX

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

DAXX (DAXX Human)

Tags

6 His

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD_IPF2.0

Plasmid

#163124

Purpose

A plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.

Depositor

Julie Biteen

Article

Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

Insert

IFP2.0

Tags

His

Expression

Bacterial

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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V621 pGEX-4T-LP - Acceptor

Plasmid

#11715

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

GST

Expression

Bacterial

Available Since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pCAM111

Plasmid

#86481

Purpose

Expresses p9-6His-tnaA

Depositor

Pamela Silver

Article

Myhrvold et al ACS Synth Biol. 2016 Aug 3.

Insert

p9-6His-tnaA

Use

Synthetic Biology

Tags

6His and tnaA

Expression

Bacterial

Promoter

pBAD

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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MP5

Plasmid

#69677

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA ugi CDA1

Expression

Bacterial

Promoter

E. coli PBAD

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pYDE007

Plasmid

#194152

Purpose

sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, CarbR. Contains mrfp nontargeting control guide sequence.

Depositor

Edward Geisinger

Article

Bai et al J Bacteriol. 2021 May 20;203(12):e005652

Insert

sgRNA with mrfp control guide sequence

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBP-ORF-xylE

Plasmid

#112780

Purpose

Encodes catechol-2,3-dioxygenase, from Pseudomonas putida TOL pWW0.

Depositor

Chris French

Article

Wicke et al bioRxiv 185108

Insert

xylE open reading frame

Expression

Bacterial

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pUCBB-pT7-eGFP

Plasmid

#32552

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Expression

Bacterial

Promoter

pT7

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pLIC-His-RGS4

Plasmid

#204847

Purpose

Express His tagged-RGS4 in bacterial cells for protein purification

Depositor

Mikel Garcia-Marcos

Article

Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

Insert

RGS4 (RGS4 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 14, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pQE60-Exp5

Plasmid

#12553

Depositor

Ian Macara

Article

Brownawell et al J Cell Biol. 2002 Jan 7. 156(1):53-64.

Insert

EXPORTIN-5 (XPO5 Human)

Tags

HIS

Expression

Bacterial

Mutation

NONE

Available Since

Oct. 2, 2006

Availability

Academic Institutions and Nonprofits only
pET22b-CPDSacI

Plasmid

#38255

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Promoter

T7

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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TRAV16

Plasmid

#123389

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV16 (TRAV16 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV13-1

Plasmid

#123386

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV13-1 (TRAV13-1 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLC133

Plasmid

#198639

Purpose

Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

Depositor

David Bikard

Article

Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

Insert

dCas9

Tags

3x-FLAG

Expression

Bacterial

Promoter

pTet

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCMW35 p21 (mouse)

Plasmid

#16478

Depositor

Bert Vogelstein

Article

el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

Insert

p21 (Cdkn1a Mouse)

Expression

Bacterial

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only