32,453 results
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Plasmid#118229PurposeEmpty E. coli - B. burgdorferi shuttle vector; hygromycin B resistantDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only
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pET28a-SENP7 (catalytic domain)
Plasmid#16360DepositorInserthuman SENP7 catalytic domain (SENP7 Human)
TagsHisExpressionBacterialMutationAmino Acid 640 to 984 catalytic domain of human S…Available SinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSG06-pLac-NRI
Plasmid#102463PurposeExpresses NRI protein in E. coli. The expression is controlled by a pLac promoter.DepositorArticleAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-ShoHELIX-KanR (BO4)
Plasmid#181795PurposepDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
ExpressionBacterialAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEA01
Plasmid#170766PurposeUtilisation of pSAM2 excision tool in Amycolatopsis sppDepositorInsertsxis, int
oriT
pac
ExpressionBacterialPromotertrcpAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp9
Plasmid#156478PurposeBacterial expression of Sars-CoV2 Nsp9 protein with His-tag and GST-tagDepositorInsertNsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pES005 (pTet-qacR wt/pQacA-deGFP)
Plasmid#69034Purposereporter with wild-type qacR on single plasmidDepositorAvailable SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTXBI-mClover-YTH domain
Plasmid#177123PurposeBacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purificationDepositorInsertYTHDC1 (YTH domain) (YTHDC1 Human)
TagsMxe GyrA intein-Chitin binding domain for protein…ExpressionBacterialMutationYTH domain only (IDR1 and IDR2 deletion)PromoterT7Available SinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSA2
Plasmid#52262PurposeBacterial expression of human 6His-tagged SUMO2 and SUMO-E1DepositorTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSKDuet16
Plasmid#41684DepositorInsertNpuDnaE intein
TagsGB1 and His-tagExpressionBacterialPromoterT7Available SinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD_A321V
Plasmid#98671PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with A321VDepositorAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP6f-RS09
Plasmid#67160Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP6f-RS09
Tags6xHis, T7 tag, and Xpress tagExpressionBacterialMutationL414T within GCaMP6fPromoterT7Available SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pLas eGFP
Plasmid#193630PurposeInducible expression of eGFP by the transcription factor complex LasR-3OC12 homoserine lactone (HSL).DepositorInserteGFP
Tags6xHisExpressionBacterialPromoterT7 RNAP promoterAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAtetO_6xHis-v5.9
Plasmid#155259PurposePlasmid for the aTc-inducible expression of the v5.9DepositorInsertv5.9
Tags6xHisExpressionBacterialPromotertet PAAvailable SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
NpuDnaEc-cGCaMP2
Plasmid#34851DepositorInsertNpuDnaEc-cGCaMP2
ExpressionBacterial and MammalianPromoterCMV, T7 lacAvailable SinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
SETDB1
Plasmid#25240PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSETDB1, SETDB1_16 (SETDB1 Human)
Tags6xHis-TEV and MHHHHHHSSGRENLYFQGExpressionBacterialPromoterT7Available SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS hBAF53a
Plasmid#17879DepositorInsertBAF53a (ACTL6A Human)
ExpressionBacterialAvailable SinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pSL0762 (pQCascade_crRNA-nt)
Plasmid#130636PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a non-targeting guide RNA.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(non-targeting)
UseCRISPR; TransposonExpressionBacterialPromoterT7Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
APneg-VAMP1d
Plasmid#168010Purposenegative selection PACE Accessory PlasmidDepositorInsertVAMP1(T29-K89)-T7PAP
ExpressionBacterialAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only