30,234 results

1. pGEX6P1-A20 (OTU, aa 1-366)

   Plasmid

   #61584

   Purpose

   Expresses human A20 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Komander et al Biochem J. 2008 Jan 1;409(1):77-85.

   Insert

   A20 (TNFAIP3 Human)
   

   Use

   Tags

   GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 367-790.

   Promoter

   tac

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET15b/GgVcl 884-1066-ECFP

   Plasmid

   #46182

   Depositor

   Susan Craig

   Insert

   Vcl 884-1066 (VCL Chicken)
   

   Use

   Tags

   ECFP and His

   Expression

   Bacterial

   Mutation

   aa 884-1066 (tail domain)

   Promoter

   T7

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pMP4639

   Plasmid

   #107771

   Purpose

   Expression of EYFP

   Depositor

   Herman Spaink

   Article

   Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

   Insert

   pLacZ-EYFP in pME6010
   

   Use

   Tags

   pLacZ-EYFP

   Expression

   Bacterial

   Mutation

   Promoter

   pLacZ

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCfuNca-Plastid

   Plasmid

   #190818

   Purpose

   Plastid targeting GFP with ble resistant gene for zeocin

   Depositor

   Peter Kroth

   Article

   Sprecher et al Algal Research. 2023 May; Vol 72: 103136

   Inserts

   Diatom plastid targeted GFP
   Bleomycin resistance gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Cylindrotheca fusiformis fcp and Nitzschia captiv…

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEB2-2ndVal mKate2

   Plasmid

   #104030

   Purpose

   Plasmid encoding 2ndVal mKate2

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   2ndVal mKate2
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   mKate2 with valine inserted in 2nd position

   Promoter

   proC

   Available since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pZSm45 ndhII

   Plasmid

   #48889

   Purpose

   Lux-regulated expression of NDH-2 enzyme

   Depositor

   Jeff Hasty

   Article

   Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

   Insert

   ndhII and LuxR
   

   Use

   Synthetic Biology

   Tags

   ssrA tag (AANDENYALAA)

   Expression

   Bacterial

   Mutation

   Promoter

   pLux

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCold I-Hero11

   Plasmid

   #187928

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero11

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero11 (C19orf53 Human)
   

   Use

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC19-TSV (linearized in LT)

   Plasmid

   #70033

   Purpose

   pUC19-TSV (linearized in LT) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the Large T-antigen coding region

   Depositor

   Mariet Feltkamp

   Article

   van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.

   Insert

   TSPyV full genome, linearized in Large T-antigen
   

   Use

   Cloning vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGex-4T-2_GATE-16

   Plasmid

   #73518

   Purpose

   For recombinant expression of human GATE-16/GABARAPL2 in E. coli

   Depositor

   Dieter Willbold

   Article

   Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

   Insert

   GATE-16 (GABARAPL2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pCJH027_SC101_pLAC_LbCas12a_Kan

    Plasmid

    #183074

    Purpose

    Wild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    LbCas12a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Wild-type

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETDuet RagA(D130N)-RagC

    Plasmid

    #99642

    Purpose

    Bacterial expression of RagA(D130N)-RagC

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D130N

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. T-IL2-12

    Plasmid

    #166564

    Purpose

    scFv of a human scaffold targeting Interleukin-2. Antigen coverage aa 21-153 of 153, full-length excl. signal peptide

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, Sortag, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET30-Npl4

    Plasmid

    #21267

    Depositor

    Hemmo Meyer

    Article

    Bruderer et al J Biol Chem. 2004 Nov 26. 279(48):49609-

    Insert

    Npl4 (Nploc4 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

14. BHMT

    Plasmid

    #64075

    Purpose

    Expression of betaine homocysteine methyltransferase

    Depositor

    Vern Schramm

    Article

    Poulin et al J Org Chem. 2015 May 15;80(10):5344-7. d

    Insert

    betaine-homocysteine methyltransferase (BHMT Synthetic, Human)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPICZa-STE2-1D4

    Plasmid

    #133436

    Purpose

    Mutant form of yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.

    Depositor

    Gerhard Wagner

    Insert

    STE2 (STE2 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C59S/C252A

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ALC1-c007

    Plasmid

    #173112

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    CHD1L (CHD1L Human)
    

    Use

    Tags

    Nanoluc

    Expression

    Bacterial

    Mutation

    L613-K879, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAM5082

    Plasmid

    #85121

    Purpose

    PkaiB-kaiC-ecfp expressed from NSII-Gm vector constructed by seamless assembly. Fusion expressed as full length fusion protein at WT levels but is 5hr long period. Used only for co-localization exp

    Depositor

    Susan Golden

    Article

    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

    Insert

    PkaiB-KaiC-ECFP
    

    Use

    Tags

    ECFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA6a-mRFP1-kanMX6

    Plasmid

    #133551

    Purpose

    Yeast fluorescent (RFP) vector with PTEF promoter.

    Depositor

    Won-Ki Huh, Erin O'Shea

    Article

    Huh et al Nature. 2003 Oct 16;425(6959):686-91. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PTPN5

    Plasmid

    #39166

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PTPN5 (PTPN5 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pET3a-Ub-K48C

    Plasmid

    #18897

    Depositor

    Cecile Pickart

    Article

    Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

    Insert

    Ubiquitin-K48C (UBC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lysine 48 is replaced with a Cysteine residue

    Promoter

    Available since

    Sept. 10, 2008

    Availability

    Academic Institutions and Nonprofits only