31,059 results

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pET-His6-SUMO-MproC145A

Plasmid

#172653

Purpose

Bacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMO

Depositor

Stephen Harrison

Article

MacDonald et al ACS Infect Dis. 2021 Sep 10;7(9):2591-25

Insert

SARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

6xHis Tag and SUMO

Expression

Bacterial

Mutation

Catalytic Cysteine 145 mutated to Alanine

Promoter

T7

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-2/Rabaptin-5:R5BD

Plasmid

#168808

Purpose

Production of recombinant GST-R5BD (Rab5-binding domain) in bacteria for GST pulldown assays to identify binding partners for Rab5 subfamily members

Depositor

Guangpu Li

Article

Qi et al Methods Mol Biol. 2015;1298:259-70. doi:

Insert

Rab5-binding domain (aa739-862) of Rabaptin-5

Tags

GST

Expression

Bacterial

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pQR

Plasmid

#48099

Purpose

Cumate-inducible expression of mCherry-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Tags

3xFLAG and mCherry

Expression

Bacterial

Promoter

PQ5

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pTA-Mob 2.0 Tp

Plasmid

#188619

Purpose

Mutated pTA-Mob 2.0 Backbone in TraJ Promoter Region

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Conjugation helper plasmid

Expression

Bacterial and Yeast

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_M337V

Plasmid

#98674

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with M337V

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Tags

6xHis

Expression

Bacterial

Mutation

M337V

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pPEPY-PF6-lacI

Plasmid

#85589

Purpose

Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae

Depositor

Jan-Willem Veening

Article

Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

Insert

lacI

Expression

Bacterial

Promoter

PF6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-156

Plasmid

#118570

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-dCas9

Plasmid

#218371

Purpose

Expression of dCas9 for CRISPR-interference

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Insert

dCas9

Use

CRISPR

Expression

Bacterial

Promoter

PtetA

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pDNA2.0 6H-TEV-KRAS G12V

Plasmid

#111850

Purpose

Expresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

Depositor

Kenneth Westover

Article

Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

Insert

KRAS G12V (KRAS Human)

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Mutation

Changed Glycine 12 to Valine

Promoter

T5

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196, D40G)-Strep

Plasmid

#196210

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, D40G)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

D40G

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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