32,947 results
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Plasmid#244831PurposeBacterial expression of catalytically inactive Cas9 (dCas9) for protein purificationDepositorInsertdCas9 (WT)
UseCRISPRTags10xHis-MBPExpressionBacterialMutationD10A/H840APromoterT7Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEF1-Pls1-sfGFP
Plasmid#205748PurposeExpresses sfGFP under IPTG inducible promoter Pls1 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Low strength.DepositorTypeEmpty backboneUseShuttle vector gram+ gram-ExpressionBacterialPromoterPls1 (Phyperspank mutant, IPTG inducible)Available SinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28_Cas9d_Plasmid
Plasmid#239445PurposeExpression plasmid codon-optimized for E. coliDepositorInsertCas9d
ExpressionBacterialPromoterT7Available SinceAug. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRK172 asyn(1-122)
Plasmid#240598PurposeOverexpress truncated alpha-Synuclein proteinDepositorAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMS01
Plasmid#230064PurposeFor allelic exchange into Bacteroides fragilis (pLGB13-based); ermC, aTC-inducible counterselection with ssbfe1; R6K ori, RP4 oriTDepositorTypeEmpty backboneUseSynthetic Biology; Allelic exchangeExpressionBacterialPromoterNoneAvailable SinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUBC-popTag-QtE-mScarlet
Plasmid#240101PurposeMammalian lentiviral vector for expressing the mScarlet-tagged 50nm-GEMs, encoded by the encapsulin protein from Quasibacillus thermotolerans and fused to a PopZ tag.DepositorInsertEncapsulin
UseLentiviralTagsmScarlet and popZExpressionBacterial and MammalianPromoterUBC promoterAvailable SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ3533 eforRed
Plasmid#239451Purposeexpress eforRed chromoproteinDepositorInserteforRed
UseCRISPR; Cell-free system using bacterial extractExpressionBacterialPromoterJ23119Available SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ3534 fwYellow
Plasmid#239452Purposeexpress fwYellow chromoproteinDepositorInsertfwYellow
UseCRISPR; Cell-free system using bacterial extractExpressionBacterialPromoterJ23119Available SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ13960 tyrosinase(Priestia megaterium)
Plasmid#239454Purposeexpress tyrosinase protein and melaninDepositorInserttyrosinase
UseCRISPR; Cell-free system using bacterial extractExpressionBacterialPromoterJ23119Available SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ14103 aeBlue-targeting_sgRNA
Plasmid#239750Purposeguide for aeBlueDepositorInsertguide targeting aeBlue
UseCRISPR; Cell-free system using bacterial extractExpressionBacterialPromoterJ23119Available SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
p300dAIL KAT LIC 1M
Plasmid#233588PurposeBacterial expression of p300 KAT enzyme with truncated autoinhibitory loopDepositorInsertp300dAIL KAT (EP300 Human)
Tags6xHis MBPExpressionBacterialMutationdeleted amino acids 1523-1554PromoterT7Available SinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTW386c
Plasmid#237321Purposeexpresses O. sativa Cpn60α Cpn60β Cpn20-2 Raf1 Raf2 RbcX Bsd2DepositorInsertO. sativa Cpn60α Cpn60β Cpn20-2 Raf1 Raf2 RbcX Bsd2
ExpressionBacterialAvailable SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMIIS644
Plasmid#238209PurposeExpresses native FpTasA TIGR locus. CmRDepositorInsertFpTasA
ExpressionBacterialAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAK004
Plasmid#233641PurposeFor bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagA.DepositorInserteSpCas9(1.1) with c-terminal VitelloTagA
Tags6-HisExpressionBacterialAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
p344_DG
Plasmid#234674PurposeGolden gate domesticated pET28b with His6-3CDepositorTypeEmpty backboneTagsHis6-3CExpressionBacterialMutationNoAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
NP-11-64-1
Plasmid#234359PurposeExpressing rubisco and prk in ΔrpiDepositorInsertR.rubrum rubisco
UseSynthetic BiologyExpressionBacterialPromoterPtacAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_N-myc
Plasmid#234045PurposeBacterial expression of N-terminally 6His-tagged N-myc; includes TEV cleavage siteDepositorAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_MAX
Plasmid#234044PurposeBacterial expression of N-terminally 6His-tagged MAX; includes TEV cleavage siteDepositorAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIA123
Plasmid#234038PurposeE. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.DepositorInsertsCas9
sgRNA
UseCRISPRExpressionBacterialPromoterPgdh and PxylAvailable SinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mGold2t
Plasmid#231766PurposeExpression of mGold2t in bacterial cellsDepositorInsertmGold2t
ExpressionBacterialMutationmGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…PromoterSynthetic promoterAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only